Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02408 and H281DRAFT_02409 are separated by 116 nucleotides H281DRAFT_02409 and H281DRAFT_02410 are separated by 160 nucleotides H281DRAFT_02410 and H281DRAFT_02411 are separated by 78 nucleotides
H281DRAFT_02408: H281DRAFT_02408 - Alginate lyase, at 579,552 to 580,682
_02408
H281DRAFT_02409: H281DRAFT_02409 - glycine dehydrogenase, at 580,799 to 583,735
_02409
H281DRAFT_02410: H281DRAFT_02410 - glycine cleavage system H protein, at 583,896 to 584,276
_02410
H281DRAFT_02411: H281DRAFT_02411 - aminomethyltransferase, at 584,355 to 585,473
_02411
Position (kb) 580
581
582
583
584 Strain fitness (log2 ratio) -2
-1
0
1
2 at 579.905 kb on - strand, within H281DRAFT_02408 at 579.913 kb on + strand, within H281DRAFT_02408 at 579.913 kb on + strand, within H281DRAFT_02408 at 579.914 kb on - strand, within H281DRAFT_02408 at 579.914 kb on - strand, within H281DRAFT_02408 at 579.977 kb on + strand, within H281DRAFT_02408 at 579.978 kb on - strand, within H281DRAFT_02408 at 579.978 kb on - strand, within H281DRAFT_02408 at 579.978 kb on - strand, within H281DRAFT_02408 at 579.978 kb on - strand, within H281DRAFT_02408 at 580.084 kb on + strand, within H281DRAFT_02408 at 580.207 kb on + strand, within H281DRAFT_02408 at 580.265 kb on - strand, within H281DRAFT_02408 at 580.265 kb on - strand, within H281DRAFT_02408 at 580.282 kb on + strand, within H281DRAFT_02408 at 580.393 kb on + strand at 580.393 kb on + strand, within H281DRAFT_02408 at 580.394 kb on - strand, within H281DRAFT_02408 at 580.394 kb on - strand, within H281DRAFT_02408 at 580.612 kb on + strand at 580.612 kb on + strand at 580.613 kb on - strand at 580.800 kb on + strand at 580.800 kb on + strand at 580.851 kb on + strand at 580.851 kb on + strand at 580.852 kb on - strand at 580.884 kb on + strand at 580.884 kb on + strand at 580.885 kb on - strand at 580.915 kb on - strand at 580.935 kb on + strand at 580.935 kb on + strand at 581.164 kb on - strand, within H281DRAFT_02409 at 581.271 kb on + strand, within H281DRAFT_02409 at 581.271 kb on + strand, within H281DRAFT_02409 at 581.272 kb on - strand, within H281DRAFT_02409 at 581.272 kb on - strand, within H281DRAFT_02409 at 581.310 kb on + strand, within H281DRAFT_02409 at 581.310 kb on + strand, within H281DRAFT_02409 at 581.311 kb on - strand at 581.376 kb on + strand, within H281DRAFT_02409 at 581.376 kb on + strand, within H281DRAFT_02409 at 581.377 kb on - strand, within H281DRAFT_02409 at 581.377 kb on - strand, within H281DRAFT_02409 at 581.377 kb on - strand, within H281DRAFT_02409 at 581.377 kb on - strand, within H281DRAFT_02409 at 581.377 kb on - strand, within H281DRAFT_02409 at 581.377 kb on - strand, within H281DRAFT_02409 at 581.412 kb on + strand, within H281DRAFT_02409 at 581.412 kb on + strand, within H281DRAFT_02409 at 581.438 kb on - strand, within H281DRAFT_02409 at 581.458 kb on - strand, within H281DRAFT_02409 at 581.646 kb on + strand, within H281DRAFT_02409 at 581.646 kb on + strand, within H281DRAFT_02409 at 581.646 kb on + strand, within H281DRAFT_02409 at 581.646 kb on + strand, within H281DRAFT_02409 at 581.725 kb on - strand, within H281DRAFT_02409 at 581.925 kb on + strand, within H281DRAFT_02409 at 581.925 kb on + strand, within H281DRAFT_02409 at 581.926 kb on - strand, within H281DRAFT_02409 at 581.926 kb on - strand, within H281DRAFT_02409 at 581.926 kb on - strand, within H281DRAFT_02409 at 581.952 kb on + strand, within H281DRAFT_02409 at 581.953 kb on - strand, within H281DRAFT_02409 at 581.953 kb on - strand, within H281DRAFT_02409 at 582.245 kb on - strand, within H281DRAFT_02409 at 582.249 kb on - strand, within H281DRAFT_02409 at 582.249 kb on - strand, within H281DRAFT_02409 at 582.646 kb on - strand, within H281DRAFT_02409 at 582.654 kb on + strand, within H281DRAFT_02409 at 582.654 kb on + strand, within H281DRAFT_02409 at 582.655 kb on - strand, within H281DRAFT_02409 at 582.655 kb on - strand, within H281DRAFT_02409 at 582.655 kb on - strand, within H281DRAFT_02409 at 582.655 kb on - strand, within H281DRAFT_02409 at 582.655 kb on - strand, within H281DRAFT_02409 at 582.655 kb on - strand, within H281DRAFT_02409 at 582.691 kb on + strand, within H281DRAFT_02409 at 582.691 kb on + strand, within H281DRAFT_02409 at 582.692 kb on - strand, within H281DRAFT_02409 at 582.695 kb on + strand, within H281DRAFT_02409 at 582.695 kb on + strand, within H281DRAFT_02409 at 582.695 kb on + strand, within H281DRAFT_02409 at 582.695 kb on + strand, within H281DRAFT_02409 at 582.695 kb on + strand, within H281DRAFT_02409 at 582.696 kb on - strand, within H281DRAFT_02409 at 582.696 kb on - strand, within H281DRAFT_02409 at 582.696 kb on - strand, within H281DRAFT_02409 at 582.840 kb on + strand, within H281DRAFT_02409 at 583.178 kb on + strand, within H281DRAFT_02409 at 583.178 kb on + strand, within H281DRAFT_02409 at 583.179 kb on - strand, within H281DRAFT_02409 at 583.179 kb on - strand, within H281DRAFT_02409 at 583.179 kb on - strand, within H281DRAFT_02409 at 583.347 kb on + strand, within H281DRAFT_02409 at 583.357 kb on - strand, within H281DRAFT_02409 at 583.357 kb on - strand, within H281DRAFT_02409 at 583.357 kb on - strand, within H281DRAFT_02409 at 583.366 kb on - strand, within H281DRAFT_02409 at 583.391 kb on - strand, within H281DRAFT_02409 at 583.422 kb on + strand, within H281DRAFT_02409 at 583.422 kb on + strand, within H281DRAFT_02409 at 583.423 kb on - strand, within H281DRAFT_02409 at 583.423 kb on - strand, within H281DRAFT_02409 at 583.423 kb on - strand, within H281DRAFT_02409 at 583.441 kb on - strand, within H281DRAFT_02409 at 583.441 kb on - strand, within H281DRAFT_02409 at 583.507 kb on + strand at 583.508 kb on - strand at 583.884 kb on + strand at 583.884 kb on + strand at 583.884 kb on + strand at 583.884 kb on + strand at 583.884 kb on + strand at 583.885 kb on - strand at 583.886 kb on + strand at 583.886 kb on + strand at 583.886 kb on + strand at 583.886 kb on + strand at 583.887 kb on - strand at 583.887 kb on - strand at 583.887 kb on - strand at 583.919 kb on - strand at 583.990 kb on + strand, within H281DRAFT_02410 at 583.991 kb on - strand, within H281DRAFT_02410 at 584.068 kb on + strand, within H281DRAFT_02410 at 584.068 kb on + strand, within H281DRAFT_02410 at 584.069 kb on - strand, within H281DRAFT_02410 at 584.069 kb on - strand, within H281DRAFT_02410 at 584.069 kb on - strand at 584.145 kb on + strand, within H281DRAFT_02410 at 584.145 kb on + strand at 584.145 kb on + strand, within H281DRAFT_02410 at 584.145 kb on + strand, within H281DRAFT_02410 at 584.146 kb on - strand, within H281DRAFT_02410 at 584.252 kb on - strand at 584.287 kb on + strand at 584.311 kb on + strand at 584.312 kb on - strand at 584.345 kb on + strand at 584.346 kb on - strand at 584.370 kb on + strand at 584.401 kb on - strand at 584.735 kb on + strand, within H281DRAFT_02411
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 579,905 - H281DRAFT_02408 0.31 -0.3 579,913 + H281DRAFT_02408 0.32 +0.7 579,913 + H281DRAFT_02408 0.32 +0.6 579,914 - H281DRAFT_02408 0.32 +0.2 579,914 - H281DRAFT_02408 0.32 +0.2 579,977 + H281DRAFT_02408 0.38 +0.4 579,978 - H281DRAFT_02408 0.38 +0.2 579,978 - H281DRAFT_02408 0.38 +0.0 579,978 - H281DRAFT_02408 0.38 +0.2 579,978 - H281DRAFT_02408 0.38 +0.2 580,084 + H281DRAFT_02408 0.47 +0.5 580,207 + H281DRAFT_02408 0.58 +0.0 580,265 - H281DRAFT_02408 0.63 -0.2 580,265 - H281DRAFT_02408 0.63 +0.2 580,282 + H281DRAFT_02408 0.65 +0.5 580,393 + -0.4 580,393 + H281DRAFT_02408 0.74 +0.4 580,394 - H281DRAFT_02408 0.74 -0.2 580,394 - H281DRAFT_02408 0.74 -0.3 580,612 + +0.8 580,612 + -0.0 580,613 - -0.4 580,800 + +0.3 580,800 + +0.3 580,851 + -0.6 580,851 + -0.9 580,852 - -1.3 580,884 + -1.0 580,884 + -1.1 580,885 - -1.2 580,915 - -0.6 580,935 + -0.8 580,935 + -1.1 581,164 - H281DRAFT_02409 0.12 -0.3 581,271 + H281DRAFT_02409 0.16 -1.0 581,271 + H281DRAFT_02409 0.16 -0.2 581,272 - H281DRAFT_02409 0.16 -2.1 581,272 - H281DRAFT_02409 0.16 -0.7 581,310 + H281DRAFT_02409 0.17 -1.0 581,310 + H281DRAFT_02409 0.17 -1.0 581,311 - -1.8 581,376 + H281DRAFT_02409 0.20 -0.5 581,376 + H281DRAFT_02409 0.20 -1.4 581,377 - H281DRAFT_02409 0.20 -0.4 581,377 - H281DRAFT_02409 0.20 -0.2 581,377 - H281DRAFT_02409 0.20 -1.3 581,377 - H281DRAFT_02409 0.20 -0.6 581,377 - H281DRAFT_02409 0.20 -0.4 581,377 - H281DRAFT_02409 0.20 -1.5 581,412 + H281DRAFT_02409 0.21 -2.1 581,412 + H281DRAFT_02409 0.21 -0.7 581,438 - H281DRAFT_02409 0.22 -0.8 581,458 - H281DRAFT_02409 0.22 +0.3 581,646 + H281DRAFT_02409 0.29 -0.8 581,646 + H281DRAFT_02409 0.29 -1.0 581,646 + H281DRAFT_02409 0.29 -0.1 581,646 + H281DRAFT_02409 0.29 -0.3 581,725 - H281DRAFT_02409 0.32 -1.6 581,925 + H281DRAFT_02409 0.38 -0.4 581,925 + H281DRAFT_02409 0.38 -1.4 581,926 - H281DRAFT_02409 0.38 -0.8 581,926 - H281DRAFT_02409 0.38 -0.6 581,926 - H281DRAFT_02409 0.38 -0.1 581,952 + H281DRAFT_02409 0.39 -0.4 581,953 - H281DRAFT_02409 0.39 +0.8 581,953 - H281DRAFT_02409 0.39 -2.3 582,245 - H281DRAFT_02409 0.49 -0.0 582,249 - H281DRAFT_02409 0.49 -0.6 582,249 - H281DRAFT_02409 0.49 -2.0 582,646 - H281DRAFT_02409 0.63 -0.7 582,654 + H281DRAFT_02409 0.63 -1.7 582,654 + H281DRAFT_02409 0.63 -0.3 582,655 - H281DRAFT_02409 0.63 -1.2 582,655 - H281DRAFT_02409 0.63 -1.0 582,655 - H281DRAFT_02409 0.63 +0.4 582,655 - H281DRAFT_02409 0.63 -0.9 582,655 - H281DRAFT_02409 0.63 -0.8 582,655 - H281DRAFT_02409 0.63 +0.0 582,691 + H281DRAFT_02409 0.64 -0.4 582,691 + H281DRAFT_02409 0.64 -2.2 582,692 - H281DRAFT_02409 0.64 -0.8 582,695 + H281DRAFT_02409 0.65 -1.1 582,695 + H281DRAFT_02409 0.65 -0.7 582,695 + H281DRAFT_02409 0.65 -0.8 582,695 + H281DRAFT_02409 0.65 -0.9 582,695 + H281DRAFT_02409 0.65 -0.5 582,696 - H281DRAFT_02409 0.65 -0.6 582,696 - H281DRAFT_02409 0.65 -0.3 582,696 - H281DRAFT_02409 0.65 -1.0 582,840 + H281DRAFT_02409 0.69 -0.7 583,178 + H281DRAFT_02409 0.81 -1.9 583,178 + H281DRAFT_02409 0.81 -0.7 583,179 - H281DRAFT_02409 0.81 -0.8 583,179 - H281DRAFT_02409 0.81 -1.3 583,179 - H281DRAFT_02409 0.81 -1.6 583,347 + H281DRAFT_02409 0.87 -0.4 583,357 - H281DRAFT_02409 0.87 -0.3 583,357 - H281DRAFT_02409 0.87 -0.7 583,357 - H281DRAFT_02409 0.87 -1.2 583,366 - H281DRAFT_02409 0.87 -1.3 583,391 - H281DRAFT_02409 0.88 -0.7 583,422 + H281DRAFT_02409 0.89 -0.5 583,422 + H281DRAFT_02409 0.89 -0.4 583,423 - H281DRAFT_02409 0.89 -0.9 583,423 - H281DRAFT_02409 0.89 -1.2 583,423 - H281DRAFT_02409 0.89 -0.2 583,441 - H281DRAFT_02409 0.90 -0.9 583,441 - H281DRAFT_02409 0.90 -1.9 583,507 + -0.5 583,508 - -0.7 583,884 + +0.0 583,884 + -0.7 583,884 + -1.1 583,884 + -0.8 583,884 + -0.7 583,885 - -0.6 583,886 + +0.6 583,886 + +1.7 583,886 + -0.4 583,886 + -1.7 583,887 - +0.5 583,887 - -0.6 583,887 - +0.1 583,919 - +0.1 583,990 + H281DRAFT_02410 0.25 -0.6 583,991 - H281DRAFT_02410 0.25 -0.9 584,068 + H281DRAFT_02410 0.45 -0.6 584,068 + H281DRAFT_02410 0.45 -1.9 584,069 - H281DRAFT_02410 0.45 -1.5 584,069 - H281DRAFT_02410 0.45 -0.3 584,069 - +2.7 584,145 + H281DRAFT_02410 0.65 -2.5 584,145 + +0.3 584,145 + H281DRAFT_02410 0.65 +0.6 584,145 + H281DRAFT_02410 0.65 -0.5 584,146 - H281DRAFT_02410 0.66 -0.8 584,252 - -0.7 584,287 + -0.6 584,311 + -1.6 584,312 - +0.3 584,345 + -1.1 584,346 - -0.3 584,370 + -1.1 584,401 - +0.0 584,735 + H281DRAFT_02411 0.34 -1.4
Or see this region's nucleotide sequence