Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02089 and H281DRAFT_02090 are separated by 46 nucleotides H281DRAFT_02090 and H281DRAFT_02091 are separated by 12 nucleotides H281DRAFT_02091 and H281DRAFT_02092 are separated by 6 nucleotides
H281DRAFT_02089: H281DRAFT_02089 - acetyl-CoA C-acetyltransferase, at 214,180 to 215,388
_02089
H281DRAFT_02090: H281DRAFT_02090 - Short-chain dehydrogenase, at 215,435 to 216,112
_02090
H281DRAFT_02091: H281DRAFT_02091 - hypothetical protein, at 216,125 to 217,261
_02091
H281DRAFT_02092: H281DRAFT_02092 - SEC-C motif-containing protein, at 217,268 to 217,744
_02092
Position (kb) 216
217
218 Strain fitness (log2 ratio) -1
0
1 at 215.277 kb on + strand at 215.394 kb on - strand at 215.397 kb on - strand at 215.397 kb on - strand at 215.503 kb on + strand, within H281DRAFT_02090 at 215.504 kb on - strand, within H281DRAFT_02090 at 215.579 kb on + strand, within H281DRAFT_02090 at 215.579 kb on + strand, within H281DRAFT_02090 at 215.675 kb on + strand, within H281DRAFT_02090 at 215.870 kb on + strand, within H281DRAFT_02090 at 215.871 kb on - strand, within H281DRAFT_02090 at 216.087 kb on - strand at 216.399 kb on + strand, within H281DRAFT_02091 at 216.400 kb on - strand, within H281DRAFT_02091 at 216.400 kb on - strand, within H281DRAFT_02091 at 216.419 kb on + strand, within H281DRAFT_02091 at 216.677 kb on - strand, within H281DRAFT_02091 at 216.701 kb on + strand, within H281DRAFT_02091 at 216.896 kb on + strand, within H281DRAFT_02091 at 216.914 kb on + strand, within H281DRAFT_02091 at 216.915 kb on - strand, within H281DRAFT_02091 at 216.915 kb on - strand, within H281DRAFT_02091 at 217.064 kb on - strand, within H281DRAFT_02091 at 217.152 kb on - strand at 217.191 kb on + strand at 217.191 kb on + strand at 217.192 kb on - strand at 217.208 kb on + strand at 217.409 kb on + strand, within H281DRAFT_02092 at 217.409 kb on + strand, within H281DRAFT_02092 at 217.472 kb on + strand, within H281DRAFT_02092 at 217.473 kb on - strand, within H281DRAFT_02092 at 217.473 kb on - strand, within H281DRAFT_02092 at 217.494 kb on - strand, within H281DRAFT_02092 at 217.643 kb on + strand at 217.643 kb on + strand, within H281DRAFT_02092 at 217.644 kb on - strand, within H281DRAFT_02092 at 217.734 kb on + strand at 217.734 kb on + strand at 217.734 kb on + strand at 217.761 kb on - strand at 217.761 kb on - strand at 217.761 kb on - strand at 217.761 kb on - strand at 217.763 kb on + strand at 217.763 kb on + strand at 217.763 kb on + strand at 217.764 kb on - strand at 217.764 kb on - strand at 217.786 kb on - strand at 217.804 kb on - strand at 217.813 kb on + strand at 217.813 kb on + strand at 217.861 kb on - strand at 218.148 kb on - strand at 218.157 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 215,277 + -0.7 215,394 - -0.4 215,397 - -0.2 215,397 - +0.0 215,503 + H281DRAFT_02090 0.10 +0.2 215,504 - H281DRAFT_02090 0.10 +1.3 215,579 + H281DRAFT_02090 0.21 -0.2 215,579 + H281DRAFT_02090 0.21 -0.1 215,675 + H281DRAFT_02090 0.35 -0.4 215,870 + H281DRAFT_02090 0.64 -0.3 215,871 - H281DRAFT_02090 0.64 +0.2 216,087 - -0.4 216,399 + H281DRAFT_02091 0.24 +0.5 216,400 - H281DRAFT_02091 0.24 -0.4 216,400 - H281DRAFT_02091 0.24 -0.6 216,419 + H281DRAFT_02091 0.26 -0.4 216,677 - H281DRAFT_02091 0.49 -0.2 216,701 + H281DRAFT_02091 0.51 +1.0 216,896 + H281DRAFT_02091 0.68 -0.2 216,914 + H281DRAFT_02091 0.69 +1.3 216,915 - H281DRAFT_02091 0.69 +0.4 216,915 - H281DRAFT_02091 0.69 +0.5 217,064 - H281DRAFT_02091 0.83 -0.2 217,152 - -0.3 217,191 + -0.0 217,191 + +0.2 217,192 - -0.3 217,208 + +0.3 217,409 + H281DRAFT_02092 0.30 +0.2 217,409 + H281DRAFT_02092 0.30 -0.0 217,472 + H281DRAFT_02092 0.43 -0.2 217,473 - H281DRAFT_02092 0.43 +0.3 217,473 - H281DRAFT_02092 0.43 +0.1 217,494 - H281DRAFT_02092 0.47 -0.6 217,643 + +0.7 217,643 + H281DRAFT_02092 0.79 +0.3 217,644 - H281DRAFT_02092 0.79 -0.2 217,734 + +0.1 217,734 + +0.5 217,734 + -0.5 217,761 - -0.6 217,761 - +0.0 217,761 - +0.9 217,761 - +0.0 217,763 + -0.1 217,763 + -0.8 217,763 + -0.0 217,764 - +0.3 217,764 - -0.1 217,786 - +0.4 217,804 - -0.4 217,813 + -0.6 217,813 + +0.7 217,861 - -0.2 218,148 - +0.1 218,157 + -0.0
Or see this region's nucleotide sequence