Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01998 and H281DRAFT_01999 overlap by 28 nucleotides H281DRAFT_01999 and H281DRAFT_02000 are separated by 114 nucleotides H281DRAFT_02000 and H281DRAFT_02001 are separated by 21 nucleotides H281DRAFT_02001 and H281DRAFT_02002 are separated by 150 nucleotides H281DRAFT_02002 and H281DRAFT_02003 overlap by 4 nucleotides
H281DRAFT_01998: H281DRAFT_01998 - tRNA (cytidine/uridine-2'-O-)-methyltransferase, at 116,745 to 117,215
_01998
H281DRAFT_01999: H281DRAFT_01999 - O-acetyl-ADP-ribose deacetylase (regulator of RNase III), contains Macro domain, at 117,188 to 117,736
_01999
H281DRAFT_02000: H281DRAFT_02000 - glycerol-3-phosphate dehydrogenase (NAD(P)+), at 117,851 to 118,849
_02000
H281DRAFT_02001: H281DRAFT_02001 - protein translocase subunit secB, at 118,871 to 119,341
_02001
H281DRAFT_02002: H281DRAFT_02002 - glutaredoxin 3, at 119,492 to 119,752
_02002
H281DRAFT_02003: H281DRAFT_02003 - Rhodanese-related sulfurtransferase, at 119,749 to 120,222
_02003
Position (kb)
117
118
119 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 116.895 kb on + strand, within H281DRAFT_01998 at 116.896 kb on - strand, within H281DRAFT_01998 at 116.905 kb on - strand, within H281DRAFT_01998 at 116.945 kb on - strand, within H281DRAFT_01998 at 117.508 kb on - strand, within H281DRAFT_01999 at 117.508 kb on - strand, within H281DRAFT_01999 at 117.649 kb on + strand, within H281DRAFT_01999 at 117.649 kb on + strand, within H281DRAFT_01999 at 117.649 kb on + strand, within H281DRAFT_01999 at 117.650 kb on - strand, within H281DRAFT_01999 at 117.725 kb on + strand at 117.725 kb on + strand at 117.725 kb on + strand at 117.725 kb on + strand at 117.725 kb on + strand at 117.725 kb on + strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.726 kb on - strand at 117.727 kb on + strand at 117.728 kb on - strand at 117.728 kb on - strand at 117.736 kb on + strand at 117.736 kb on + strand at 117.736 kb on + strand at 117.737 kb on - strand at 117.737 kb on - strand at 117.805 kb on + strand at 117.805 kb on + strand at 117.806 kb on - strand at 117.852 kb on + strand at 118.295 kb on + strand, within H281DRAFT_02000 at 118.296 kb on - strand, within H281DRAFT_02000 at 118.319 kb on - strand, within H281DRAFT_02000 at 118.320 kb on + strand, within H281DRAFT_02000 at 118.320 kb on + strand, within H281DRAFT_02000 at 118.321 kb on - strand, within H281DRAFT_02000 at 118.321 kb on - strand, within H281DRAFT_02000 at 118.321 kb on - strand at 118.543 kb on + strand, within H281DRAFT_02000 at 118.544 kb on - strand, within H281DRAFT_02000 at 118.710 kb on + strand, within H281DRAFT_02000 at 118.849 kb on + strand at 118.872 kb on + strand at 118.872 kb on + strand at 118.872 kb on + strand at 118.873 kb on - strand at 118.873 kb on - strand at 118.873 kb on - strand at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 118.936 kb on - strand, within H281DRAFT_02001 at 119.360 kb on + strand at 119.361 kb on - strand at 119.361 kb on - strand at 119.361 kb on - strand at 119.361 kb on - strand at 119.361 kb on - strand at 119.364 kb on + strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.365 kb on - strand at 119.370 kb on + strand at 119.403 kb on + strand at 119.404 kb on - strand at 119.404 kb on - strand at 119.404 kb on - strand at 119.404 kb on - strand at 119.404 kb on - strand at 119.453 kb on - strand at 119.453 kb on - strand at 119.733 kb on + strand at 119.733 kb on + strand at 119.733 kb on + strand at 119.733 kb on + strand at 119.734 kb on - strand at 119.759 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 116,895 + H281DRAFT_01998 0.32 +0.7 116,896 - H281DRAFT_01998 0.32 -0.0 116,905 - H281DRAFT_01998 0.34 -0.7 116,945 - H281DRAFT_01998 0.42 -0.0 117,508 - H281DRAFT_01999 0.58 +0.6 117,508 - H281DRAFT_01999 0.58 +0.2 117,649 + H281DRAFT_01999 0.84 -0.7 117,649 + H281DRAFT_01999 0.84 +0.2 117,649 + H281DRAFT_01999 0.84 +0.0 117,650 - H281DRAFT_01999 0.84 -0.1 117,725 + +0.0 117,725 + +0.5 117,725 + +0.2 117,725 + -0.2 117,725 + +0.3 117,725 + -0.2 117,726 - +0.6 117,726 - +0.3 117,726 - -0.3 117,726 - -1.1 117,726 - -0.0 117,726 - -0.1 117,726 - +0.1 117,726 - +0.2 117,726 - -0.7 117,726 - -0.4 117,727 + +0.0 117,728 - -0.1 117,728 - +0.5 117,736 + -0.3 117,736 + +0.2 117,736 + +0.1 117,737 - -0.7 117,737 - +0.2 117,805 + -1.5 117,805 + -0.8 117,806 - -0.2 117,852 + +0.3 118,295 + H281DRAFT_02000 0.44 -1.9 118,296 - H281DRAFT_02000 0.45 -3.8 118,319 - H281DRAFT_02000 0.47 -1.6 118,320 + H281DRAFT_02000 0.47 -2.1 118,320 + H281DRAFT_02000 0.47 -2.7 118,321 - H281DRAFT_02000 0.47 +0.2 118,321 - H281DRAFT_02000 0.47 -3.4 118,321 - +0.7 118,543 + H281DRAFT_02000 0.69 -3.1 118,544 - H281DRAFT_02000 0.69 -3.1 118,710 + H281DRAFT_02000 0.86 -2.1 118,849 + -2.1 118,872 + -3.3 118,872 + -2.1 118,872 + -2.1 118,873 - -3.1 118,873 - +0.4 118,873 - -3.3 118,936 - H281DRAFT_02001 0.14 -0.1 118,936 - H281DRAFT_02001 0.14 +0.3 118,936 - H281DRAFT_02001 0.14 +0.6 118,936 - H281DRAFT_02001 0.14 -1.0 118,936 - H281DRAFT_02001 0.14 +0.2 118,936 - H281DRAFT_02001 0.14 +0.2 118,936 - H281DRAFT_02001 0.14 -0.1 118,936 - H281DRAFT_02001 0.14 +0.6 119,360 + -0.6 119,361 - +0.2 119,361 - +0.4 119,361 - -0.1 119,361 - +0.2 119,361 - +0.0 119,364 + -1.4 119,365 - -0.3 119,365 - -0.6 119,365 - -0.2 119,365 - +0.2 119,365 - +0.2 119,365 - -0.3 119,365 - +0.0 119,365 - +0.9 119,370 + +0.2 119,403 + +0.1 119,404 - -0.6 119,404 - +0.8 119,404 - -0.2 119,404 - -0.0 119,404 - +0.2 119,453 - -0.3 119,453 - +0.1 119,733 + +0.1 119,733 + -0.0 119,733 + -0.1 119,733 + -0.5 119,734 - -0.3 119,759 - +0.8
Or see this region's nucleotide sequence