Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01933 and H281DRAFT_01934 are separated by 6 nucleotides H281DRAFT_01934 and H281DRAFT_01935 are separated by 87 nucleotides H281DRAFT_01935 and H281DRAFT_01936 overlap by 1 nucleotides H281DRAFT_01936 and H281DRAFT_01937 are separated by 60 nucleotides
H281DRAFT_01933: H281DRAFT_01933 - A/G-specific DNA-adenine glycosylase, at 49,821 to 50,969
_01933
H281DRAFT_01934: H281DRAFT_01934 - DNA-(apurinic or apyrimidinic site) lyase, at 50,976 to 51,806
_01934
H281DRAFT_01935: H281DRAFT_01935 - Tetratricopeptide repeat-containing protein, at 51,894 to 53,735
_01935
H281DRAFT_01936: H281DRAFT_01936 - outer membrane lipoprotein LolB, at 53,735 to 54,367
_01936
H281DRAFT_01937: H281DRAFT_01937 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, at 54,428 to 55,309
_01937
Position (kb)
51
52
53
54 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 50.907 kb on + strand at 51.076 kb on + strand, within H281DRAFT_01934 at 51.076 kb on + strand, within H281DRAFT_01934 at 51.077 kb on - strand, within H281DRAFT_01934 at 51.086 kb on - strand at 51.202 kb on + strand, within H281DRAFT_01934 at 51.207 kb on + strand, within H281DRAFT_01934 at 51.208 kb on - strand, within H281DRAFT_01934 at 51.275 kb on - strand, within H281DRAFT_01934 at 51.278 kb on + strand, within H281DRAFT_01934 at 51.278 kb on + strand, within H281DRAFT_01934 at 51.278 kb on + strand, within H281DRAFT_01934 at 51.279 kb on - strand, within H281DRAFT_01934 at 51.279 kb on - strand, within H281DRAFT_01934 at 51.279 kb on - strand, within H281DRAFT_01934 at 51.279 kb on - strand, within H281DRAFT_01934 at 51.279 kb on - strand, within H281DRAFT_01934 at 51.279 kb on - strand, within H281DRAFT_01934 at 51.279 kb on - strand, within H281DRAFT_01934 at 51.632 kb on - strand, within H281DRAFT_01934 at 51.677 kb on + strand, within H281DRAFT_01934 at 51.677 kb on + strand, within H281DRAFT_01934 at 51.678 kb on - strand, within H281DRAFT_01934 at 51.719 kb on + strand, within H281DRAFT_01934 at 51.757 kb on + strand at 51.757 kb on + strand at 51.757 kb on + strand at 51.758 kb on - strand at 51.758 kb on - strand at 51.758 kb on - strand at 51.758 kb on - strand at 51.810 kb on + strand at 51.810 kb on + strand at 51.810 kb on + strand at 51.810 kb on + strand at 51.811 kb on - strand at 51.811 kb on - strand at 51.811 kb on - strand at 51.811 kb on - strand at 51.811 kb on - strand at 51.811 kb on - strand at 51.846 kb on + strand at 51.846 kb on + strand at 51.851 kb on + strand at 51.863 kb on + strand at 51.863 kb on + strand at 51.864 kb on - strand at 52.184 kb on + strand, within H281DRAFT_01935 at 52.329 kb on + strand, within H281DRAFT_01935 at 52.782 kb on + strand, within H281DRAFT_01935 at 52.920 kb on + strand, within H281DRAFT_01935 at 52.920 kb on + strand, within H281DRAFT_01935 at 52.933 kb on - strand, within H281DRAFT_01935 at 52.980 kb on + strand, within H281DRAFT_01935 at 52.980 kb on + strand, within H281DRAFT_01935 at 52.980 kb on + strand, within H281DRAFT_01935 at 52.980 kb on + strand, within H281DRAFT_01935 at 52.982 kb on + strand, within H281DRAFT_01935 at 52.982 kb on + strand, within H281DRAFT_01935 at 52.982 kb on + strand, within H281DRAFT_01935 at 52.982 kb on + strand, within H281DRAFT_01935 at 52.982 kb on + strand, within H281DRAFT_01935 at 52.982 kb on + strand, within H281DRAFT_01935 at 52.982 kb on + strand, within H281DRAFT_01935 at 52.982 kb on + strand, within H281DRAFT_01935 at 52.987 kb on - strand, within H281DRAFT_01935 at 53.041 kb on + strand, within H281DRAFT_01935 at 53.070 kb on + strand, within H281DRAFT_01935 at 53.070 kb on + strand, within H281DRAFT_01935 at 53.070 kb on + strand, within H281DRAFT_01935 at 53.070 kb on + strand at 53.071 kb on - strand, within H281DRAFT_01935 at 53.071 kb on - strand, within H281DRAFT_01935 at 53.391 kb on + strand, within H281DRAFT_01935 at 53.392 kb on - strand, within H281DRAFT_01935 at 53.534 kb on + strand, within H281DRAFT_01935 at 53.535 kb on - strand, within H281DRAFT_01935 at 53.568 kb on + strand at 53.569 kb on - strand at 53.740 kb on + strand at 53.740 kb on + strand at 53.740 kb on + strand at 53.811 kb on + strand, within H281DRAFT_01936 at 53.924 kb on + strand, within H281DRAFT_01936 at 54.269 kb on + strand, within H281DRAFT_01936 at 54.354 kb on + strand at 54.365 kb on + strand at 54.365 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 50,907 + -0.4 51,076 + H281DRAFT_01934 0.12 -0.3 51,076 + H281DRAFT_01934 0.12 -0.9 51,077 - H281DRAFT_01934 0.12 +0.7 51,086 - +0.2 51,202 + H281DRAFT_01934 0.27 +0.2 51,207 + H281DRAFT_01934 0.28 +0.1 51,208 - H281DRAFT_01934 0.28 -0.1 51,275 - H281DRAFT_01934 0.36 -0.2 51,278 + H281DRAFT_01934 0.36 -0.2 51,278 + H281DRAFT_01934 0.36 +0.4 51,278 + H281DRAFT_01934 0.36 -0.5 51,279 - H281DRAFT_01934 0.36 +0.7 51,279 - H281DRAFT_01934 0.36 +0.2 51,279 - H281DRAFT_01934 0.36 +1.8 51,279 - H281DRAFT_01934 0.36 +0.2 51,279 - H281DRAFT_01934 0.36 -0.2 51,279 - H281DRAFT_01934 0.36 +0.5 51,279 - H281DRAFT_01934 0.36 -0.4 51,632 - H281DRAFT_01934 0.79 -0.2 51,677 + H281DRAFT_01934 0.84 -0.8 51,677 + H281DRAFT_01934 0.84 -0.6 51,678 - H281DRAFT_01934 0.84 +0.3 51,719 + H281DRAFT_01934 0.89 +0.1 51,757 + +0.2 51,757 + +0.1 51,757 + +0.1 51,758 - +0.3 51,758 - +0.3 51,758 - +0.6 51,758 - +0.1 51,810 + +0.1 51,810 + +1.0 51,810 + -0.5 51,810 + +0.5 51,811 - +1.0 51,811 - +0.3 51,811 - +1.1 51,811 - +0.7 51,811 - +0.8 51,811 - -0.2 51,846 + +0.0 51,846 + +1.1 51,851 + +0.2 51,863 + +0.5 51,863 + +0.8 51,864 - +0.5 52,184 + H281DRAFT_01935 0.16 -0.7 52,329 + H281DRAFT_01935 0.24 -0.5 52,782 + H281DRAFT_01935 0.48 -1.5 52,920 + H281DRAFT_01935 0.56 -0.8 52,920 + H281DRAFT_01935 0.56 -3.8 52,933 - H281DRAFT_01935 0.56 +0.4 52,980 + H281DRAFT_01935 0.59 -3.0 52,980 + H281DRAFT_01935 0.59 +0.2 52,980 + H281DRAFT_01935 0.59 -0.6 52,980 + H281DRAFT_01935 0.59 -0.8 52,982 + H281DRAFT_01935 0.59 -0.9 52,982 + H281DRAFT_01935 0.59 -0.3 52,982 + H281DRAFT_01935 0.59 -1.9 52,982 + H281DRAFT_01935 0.59 -0.8 52,982 + H281DRAFT_01935 0.59 -1.5 52,982 + H281DRAFT_01935 0.59 -2.1 52,982 + H281DRAFT_01935 0.59 -2.7 52,982 + H281DRAFT_01935 0.59 -1.3 52,987 - H281DRAFT_01935 0.59 -0.8 53,041 + H281DRAFT_01935 0.62 -2.1 53,070 + H281DRAFT_01935 0.64 -0.2 53,070 + H281DRAFT_01935 0.64 -0.5 53,070 + H281DRAFT_01935 0.64 -0.5 53,070 + -0.8 53,071 - H281DRAFT_01935 0.64 -1.5 53,071 - H281DRAFT_01935 0.64 -1.5 53,391 + H281DRAFT_01935 0.81 -3.4 53,392 - H281DRAFT_01935 0.81 -0.8 53,534 + H281DRAFT_01935 0.89 -0.2 53,535 - H281DRAFT_01935 0.89 -0.2 53,568 + -0.5 53,569 - -1.7 53,740 + +0.7 53,740 + -0.1 53,740 + +1.0 53,811 + H281DRAFT_01936 0.12 -0.1 53,924 + H281DRAFT_01936 0.30 +0.1 54,269 + H281DRAFT_01936 0.84 -0.9 54,354 + +1.5 54,365 + -1.3 54,365 + +0.1
Or see this region's nucleotide sequence