Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_01201

Experiment: 4-Methyl-2-oxopentanoic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_01199 and H281DRAFT_01200 overlap by 4 nucleotidesH281DRAFT_01200 and H281DRAFT_01201 overlap by 4 nucleotidesH281DRAFT_01201 and H281DRAFT_01202 are separated by 28 nucleotides H281DRAFT_01199: H281DRAFT_01199 - Enoyl-CoA hydratase/carnithine racemase, at 36,599 to 37,759 _01199 H281DRAFT_01200: H281DRAFT_01200 - short chain enoyl-CoA hydratase (EC 4.2.1.17), at 37,756 to 38,592 _01200 H281DRAFT_01201: H281DRAFT_01201 - 3-hydroxyisobutyrate dehydrogenase, at 38,589 to 39,518 _01201 H281DRAFT_01202: H281DRAFT_01202 - methylmalonate-semialdehyde dehydrogenase [acylating], at 39,547 to 41,055 _01202 Position (kb) 38 39 40Strain fitness (log2 ratio) -2 -1 0 1at 38.447 kb on - strand, within H281DRAFT_01200at 38.576 kb on + strandat 38.648 kb on - strandat 38.864 kb on + strand, within H281DRAFT_01201at 38.867 kb on - strand, within H281DRAFT_01201at 38.906 kb on + strand, within H281DRAFT_01201at 38.907 kb on - strand, within H281DRAFT_01201at 39.092 kb on - strand, within H281DRAFT_01201at 39.436 kb on - strandat 40.012 kb on - strand, within H281DRAFT_01202

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Per-strain Table

Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C)
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38,447 - H281DRAFT_01200 0.83 -2.0
38,576 + -1.8
38,648 - -0.2
38,864 + H281DRAFT_01201 0.30 -0.9
38,867 - H281DRAFT_01201 0.30 -0.7
38,906 + H281DRAFT_01201 0.34 -2.0
38,907 - H281DRAFT_01201 0.34 -1.5
39,092 - H281DRAFT_01201 0.54 -1.8
39,436 - -1.3
40,012 - H281DRAFT_01202 0.31 +0.3

Or see this region's nucleotide sequence