Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00621 and H281DRAFT_00622 are separated by 4 nucleotides H281DRAFT_00622 and H281DRAFT_00623 are separated by 36 nucleotides H281DRAFT_00623 and H281DRAFT_00624 overlap by 4 nucleotides H281DRAFT_00624 and H281DRAFT_00625 are separated by 111 nucleotides
H281DRAFT_00621: H281DRAFT_00621 - 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, at 697,891 to 698,355
_00621
H281DRAFT_00622: H281DRAFT_00622 - acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase, at 698,360 to 699,232
_00622
H281DRAFT_00623: H281DRAFT_00623 - lipid-A-disaccharide synthase, at 699,269 to 700,405
_00623
H281DRAFT_00624: H281DRAFT_00624 - RNase HII, at 700,402 to 701,247
_00624
H281DRAFT_00625: H281DRAFT_00625 - RNA methyltransferase, TrmH family, at 701,359 to 702,186
_00625
Position (kb)
699
700
701 Strain fitness (log2 ratio)
-1
0
1 at 700.566 kb on + strand, within H281DRAFT_00624 at 700.567 kb on - strand, within H281DRAFT_00624 at 700.567 kb on - strand, within H281DRAFT_00624 at 700.706 kb on + strand, within H281DRAFT_00624 at 700.706 kb on + strand, within H281DRAFT_00624 at 700.706 kb on + strand, within H281DRAFT_00624 at 700.706 kb on + strand, within H281DRAFT_00624 at 700.708 kb on + strand, within H281DRAFT_00624 at 700.709 kb on - strand, within H281DRAFT_00624 at 700.847 kb on + strand, within H281DRAFT_00624 at 700.996 kb on + strand, within H281DRAFT_00624 at 700.996 kb on + strand, within H281DRAFT_00624 at 701.279 kb on + strand at 701.279 kb on + strand at 701.338 kb on - strand at 701.338 kb on - strand at 701.393 kb on - strand at 701.393 kb on - strand at 701.395 kb on - strand at 701.395 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 700,566 + H281DRAFT_00624 0.19 +0.0 700,567 - H281DRAFT_00624 0.20 -0.9 700,567 - H281DRAFT_00624 0.20 +0.4 700,706 + H281DRAFT_00624 0.36 -0.2 700,706 + H281DRAFT_00624 0.36 -0.4 700,706 + H281DRAFT_00624 0.36 +0.1 700,706 + H281DRAFT_00624 0.36 +0.5 700,708 + H281DRAFT_00624 0.36 +0.0 700,709 - H281DRAFT_00624 0.36 -1.0 700,847 + H281DRAFT_00624 0.53 -1.1 700,996 + H281DRAFT_00624 0.70 +0.1 700,996 + H281DRAFT_00624 0.70 +0.2 701,279 + +0.5 701,279 + -0.4 701,338 - +0.8 701,338 - +0.3 701,393 - -0.6 701,393 - -0.7 701,395 - -0.4 701,395 - +0.1
Or see this region's nucleotide sequence