Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00574 and H281DRAFT_00575 are separated by 140 nucleotides H281DRAFT_00575 and H281DRAFT_00576 are separated by 147 nucleotides H281DRAFT_00576 and H281DRAFT_00577 are separated by 100 nucleotides
H281DRAFT_00574: H281DRAFT_00574 - transcriptional regulator, AraC family, at 639,989 to 640,900
_00574
H281DRAFT_00575: H281DRAFT_00575 - Uncharacterized conserved protein YtfP, gamma-glutamylcyclotransferase (GGCT)/AIG2-like family, at 641,041 to 641,442
_00575
H281DRAFT_00576: H281DRAFT_00576 - malate synthase, at 641,590 to 643,191
_00576
H281DRAFT_00577: H281DRAFT_00577 - 2-haloacid dehalogenase, at 643,292 to 644,161
_00577
Position (kb)
641
642
643
644 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 640.602 kb on + strand, within H281DRAFT_00574 at 640.603 kb on - strand, within H281DRAFT_00574 at 640.603 kb on - strand, within H281DRAFT_00574 at 640.737 kb on + strand, within H281DRAFT_00574 at 640.737 kb on + strand, within H281DRAFT_00574 at 640.737 kb on + strand, within H281DRAFT_00574 at 640.737 kb on + strand, within H281DRAFT_00574 at 640.738 kb on - strand, within H281DRAFT_00574 at 640.738 kb on - strand, within H281DRAFT_00574 at 640.763 kb on - strand, within H281DRAFT_00574 at 640.763 kb on - strand, within H281DRAFT_00574 at 640.763 kb on - strand, within H281DRAFT_00574 at 640.763 kb on - strand, within H281DRAFT_00574 at 640.968 kb on + strand at 640.968 kb on + strand at 640.968 kb on + strand at 640.969 kb on - strand at 640.996 kb on + strand at 640.997 kb on - strand at 640.997 kb on - strand at 641.019 kb on + strand at 641.057 kb on + strand at 641.057 kb on + strand at 641.057 kb on + strand at 641.057 kb on + strand at 641.058 kb on - strand at 641.058 kb on - strand at 641.058 kb on - strand at 641.060 kb on - strand at 641.234 kb on - strand, within H281DRAFT_00575 at 641.284 kb on + strand, within H281DRAFT_00575 at 641.462 kb on + strand at 641.462 kb on + strand at 641.462 kb on + strand at 641.567 kb on + strand at 641.567 kb on + strand at 641.567 kb on + strand at 641.567 kb on + strand at 641.604 kb on - strand at 641.678 kb on + strand at 641.679 kb on - strand at 641.689 kb on - strand at 641.809 kb on + strand, within H281DRAFT_00576 at 641.901 kb on - strand, within H281DRAFT_00576 at 642.254 kb on + strand, within H281DRAFT_00576 at 642.351 kb on - strand, within H281DRAFT_00576 at 642.351 kb on - strand, within H281DRAFT_00576 at 642.382 kb on - strand, within H281DRAFT_00576 at 642.382 kb on - strand, within H281DRAFT_00576 at 642.382 kb on - strand, within H281DRAFT_00576 at 642.382 kb on - strand, within H281DRAFT_00576 at 642.398 kb on + strand, within H281DRAFT_00576 at 642.551 kb on + strand, within H281DRAFT_00576 at 642.552 kb on - strand, within H281DRAFT_00576 at 642.552 kb on - strand, within H281DRAFT_00576 at 642.558 kb on - strand, within H281DRAFT_00576 at 642.692 kb on - strand, within H281DRAFT_00576 at 642.723 kb on + strand, within H281DRAFT_00576 at 642.723 kb on + strand, within H281DRAFT_00576 at 642.724 kb on - strand, within H281DRAFT_00576 at 642.724 kb on - strand, within H281DRAFT_00576 at 642.725 kb on + strand, within H281DRAFT_00576 at 642.726 kb on - strand, within H281DRAFT_00576 at 642.726 kb on - strand, within H281DRAFT_00576 at 642.726 kb on - strand, within H281DRAFT_00576 at 643.243 kb on + strand at 643.260 kb on + strand at 643.261 kb on - strand at 643.314 kb on - strand at 643.557 kb on + strand, within H281DRAFT_00577 at 643.558 kb on - strand, within H281DRAFT_00577 at 643.579 kb on - strand, within H281DRAFT_00577 at 643.858 kb on - strand, within H281DRAFT_00577 at 643.858 kb on - strand, within H281DRAFT_00577 at 643.858 kb on - strand, within H281DRAFT_00577 at 644.007 kb on + strand, within H281DRAFT_00577 at 644.112 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 640,602 + H281DRAFT_00574 0.67 -1.3 640,603 - H281DRAFT_00574 0.67 +0.1 640,603 - H281DRAFT_00574 0.67 -0.1 640,737 + H281DRAFT_00574 0.82 -0.3 640,737 + H281DRAFT_00574 0.82 +0.3 640,737 + H281DRAFT_00574 0.82 +0.4 640,737 + H281DRAFT_00574 0.82 +0.4 640,738 - H281DRAFT_00574 0.82 +0.3 640,738 - H281DRAFT_00574 0.82 +0.4 640,763 - H281DRAFT_00574 0.85 +0.5 640,763 - H281DRAFT_00574 0.85 +0.1 640,763 - H281DRAFT_00574 0.85 +0.2 640,763 - H281DRAFT_00574 0.85 +0.1 640,968 + -0.2 640,968 + +0.1 640,968 + -0.0 640,969 - -1.1 640,996 + +0.3 640,997 - +0.5 640,997 - -0.6 641,019 + +1.0 641,057 + +0.1 641,057 + -0.2 641,057 + -0.2 641,057 + +0.7 641,058 - -0.2 641,058 - +1.9 641,058 - -0.1 641,060 - -0.7 641,234 - H281DRAFT_00575 0.48 -0.0 641,284 + H281DRAFT_00575 0.60 -0.2 641,462 + +0.5 641,462 + -0.1 641,462 + -0.0 641,567 + -0.2 641,567 + -0.5 641,567 + +0.3 641,567 + +0.9 641,604 - -1.6 641,678 + -0.3 641,679 - -2.4 641,689 - -1.7 641,809 + H281DRAFT_00576 0.14 -2.5 641,901 - H281DRAFT_00576 0.19 -2.0 642,254 + H281DRAFT_00576 0.41 -2.0 642,351 - H281DRAFT_00576 0.48 -2.2 642,351 - H281DRAFT_00576 0.48 -0.4 642,382 - H281DRAFT_00576 0.49 -1.9 642,382 - H281DRAFT_00576 0.49 -1.1 642,382 - H281DRAFT_00576 0.49 -1.2 642,382 - H281DRAFT_00576 0.49 -1.1 642,398 + H281DRAFT_00576 0.50 -1.8 642,551 + H281DRAFT_00576 0.60 -0.9 642,552 - H281DRAFT_00576 0.60 -1.6 642,552 - H281DRAFT_00576 0.60 -1.6 642,558 - H281DRAFT_00576 0.60 -1.1 642,692 - H281DRAFT_00576 0.69 -1.9 642,723 + H281DRAFT_00576 0.71 -2.5 642,723 + H281DRAFT_00576 0.71 -2.2 642,724 - H281DRAFT_00576 0.71 -0.8 642,724 - H281DRAFT_00576 0.71 +0.2 642,725 + H281DRAFT_00576 0.71 -1.2 642,726 - H281DRAFT_00576 0.71 -1.6 642,726 - H281DRAFT_00576 0.71 -2.4 642,726 - H281DRAFT_00576 0.71 -2.9 643,243 + -0.9 643,260 + -1.7 643,261 - -0.2 643,314 - +0.8 643,557 + H281DRAFT_00577 0.30 -1.7 643,558 - H281DRAFT_00577 0.31 +0.6 643,579 - H281DRAFT_00577 0.33 +0.9 643,858 - H281DRAFT_00577 0.65 +0.6 643,858 - H281DRAFT_00577 0.65 +2.1 643,858 - H281DRAFT_00577 0.65 -0.1 644,007 + H281DRAFT_00577 0.82 -1.5 644,112 + -1.3
Or see this region's nucleotide sequence