Experiment: 4-Methyl-2-oxopentanoic (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00311 and H281DRAFT_00312 are separated by 52 nucleotides H281DRAFT_00312 and H281DRAFT_00313 are separated by 38 nucleotides H281DRAFT_00313 and H281DRAFT_00314 overlap by 38 nucleotides
H281DRAFT_00311: H281DRAFT_00311 - DNA polymerase III, alpha subunit, at 336,155 to 339,745
_00311
H281DRAFT_00312: H281DRAFT_00312 - Glycosyltransferase involved in cell wall bisynthesis, at 339,798 to 340,562
_00312
H281DRAFT_00313: H281DRAFT_00313 - hypothetical protein, at 340,601 to 340,849
_00313
H281DRAFT_00314: H281DRAFT_00314 - ATP-binding cassette, subfamily B, MsbA, at 340,812 to 342,605
_00314
Position (kb) 340
341 Strain fitness (log2 ratio) -1
0
1
2 at 339.741 kb on - strand at 339.847 kb on + strand at 339.847 kb on + strand at 339.850 kb on + strand at 339.923 kb on - strand, within H281DRAFT_00312 at 340.018 kb on + strand, within H281DRAFT_00312 at 340.038 kb on + strand, within H281DRAFT_00312 at 340.562 kb on + strand at 340.563 kb on - strand at 340.655 kb on + strand, within H281DRAFT_00313 at 340.655 kb on + strand, within H281DRAFT_00313 at 340.655 kb on + strand, within H281DRAFT_00313 at 340.655 kb on + strand, within H281DRAFT_00313 at 340.656 kb on - strand, within H281DRAFT_00313 at 340.732 kb on + strand, within H281DRAFT_00313
Per-strain Table
Position Strand Gene LocusTag Fraction 4-Methyl-2-oxopentanoic (C) remove 339,741 - -1.4 339,847 + +2.3 339,847 + +1.2 339,850 + -0.6 339,923 - H281DRAFT_00312 0.16 +0.3 340,018 + H281DRAFT_00312 0.29 +0.2 340,038 + H281DRAFT_00312 0.31 +0.7 340,562 + +1.5 340,563 - -0.1 340,655 + H281DRAFT_00313 0.22 +1.0 340,655 + H281DRAFT_00313 0.22 -0.1 340,655 + H281DRAFT_00313 0.22 +0.9 340,655 + H281DRAFT_00313 0.22 -0.2 340,656 - H281DRAFT_00313 0.22 +0.4 340,732 + H281DRAFT_00313 0.53 +0.8
Or see this region's nucleotide sequence