Experiment: Casamino Acids (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26260 and BWI76_RS26265 overlap by 26 nucleotides BWI76_RS26265 and BWI76_RS26270 are separated by 42 nucleotides BWI76_RS26270 and BWI76_RS26275 are separated by 50 nucleotides BWI76_RS26275 and BWI76_RS26280 are separated by 121 nucleotides BWI76_RS26280 and BWI76_RS26285 are separated by 23 nucleotides
BWI76_RS26260: BWI76_RS26260 - hypothetical protein, at 5,436,830 to 5,437,468
_RS26260
BWI76_RS26265: BWI76_RS26265 - hypothetical protein, at 5,437,443 to 5,437,673
_RS26265
BWI76_RS26270: BWI76_RS26270 - N-acetyltransferase, at 5,437,716 to 5,438,207
_RS26270
BWI76_RS26275: BWI76_RS26275 - gamma-glutamyltransferase, at 5,438,258 to 5,440,000
_RS26275
BWI76_RS26280: BWI76_RS26280 - hypothetical protein, at 5,440,122 to 5,440,442
_RS26280
BWI76_RS26285: BWI76_RS26285 - glycerophosphodiester phosphodiesterase, at 5,440,466 to 5,441,206
_RS26285
Position (kb) 5438
5439
5440
5441 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5437.335 kb on - strand, within BWI76_RS26260 at 5437.736 kb on - strand at 5437.875 kb on - strand, within BWI76_RS26270 at 5437.894 kb on + strand, within BWI76_RS26270 at 5437.929 kb on - strand, within BWI76_RS26270 at 5437.936 kb on - strand, within BWI76_RS26270 at 5437.990 kb on + strand, within BWI76_RS26270 at 5438.044 kb on - strand, within BWI76_RS26270 at 5438.074 kb on + strand, within BWI76_RS26270 at 5438.182 kb on + strand at 5438.212 kb on + strand at 5438.227 kb on - strand at 5438.248 kb on - strand at 5438.256 kb on + strand at 5438.264 kb on - strand at 5438.264 kb on - strand at 5438.264 kb on - strand at 5438.309 kb on - strand at 5438.309 kb on - strand at 5438.337 kb on - strand at 5438.763 kb on + strand, within BWI76_RS26275 at 5438.773 kb on + strand, within BWI76_RS26275 at 5438.773 kb on + strand, within BWI76_RS26275 at 5439.138 kb on - strand, within BWI76_RS26275 at 5439.140 kb on + strand, within BWI76_RS26275 at 5439.140 kb on + strand, within BWI76_RS26275 at 5439.148 kb on - strand, within BWI76_RS26275 at 5439.148 kb on - strand, within BWI76_RS26275 at 5439.212 kb on + strand, within BWI76_RS26275 at 5439.231 kb on + strand, within BWI76_RS26275 at 5439.267 kb on + strand, within BWI76_RS26275 at 5439.417 kb on - strand, within BWI76_RS26275 at 5439.503 kb on + strand, within BWI76_RS26275 at 5439.540 kb on - strand, within BWI76_RS26275 at 5439.572 kb on - strand, within BWI76_RS26275 at 5439.678 kb on + strand, within BWI76_RS26275 at 5439.686 kb on - strand, within BWI76_RS26275 at 5439.742 kb on + strand, within BWI76_RS26275 at 5439.854 kb on + strand at 5439.897 kb on + strand at 5439.897 kb on + strand at 5439.905 kb on - strand at 5439.942 kb on - strand at 5439.963 kb on + strand at 5440.096 kb on - strand at 5440.112 kb on + strand at 5440.115 kb on - strand at 5440.178 kb on + strand, within BWI76_RS26280 at 5440.197 kb on - strand, within BWI76_RS26280 at 5440.218 kb on - strand, within BWI76_RS26280 at 5440.293 kb on + strand, within BWI76_RS26280 at 5440.293 kb on + strand, within BWI76_RS26280 at 5440.601 kb on - strand, within BWI76_RS26285 at 5440.635 kb on - strand, within BWI76_RS26285 at 5440.635 kb on - strand, within BWI76_RS26285 at 5440.698 kb on + strand, within BWI76_RS26285 at 5440.943 kb on - strand, within BWI76_RS26285
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino Acids (N) remove 5,437,335 - BWI76_RS26260 0.79 +0.0 5,437,736 - +0.8 5,437,875 - BWI76_RS26270 0.32 +0.8 5,437,894 + BWI76_RS26270 0.36 +2.4 5,437,929 - BWI76_RS26270 0.43 +0.5 5,437,936 - BWI76_RS26270 0.45 +2.4 5,437,990 + BWI76_RS26270 0.56 -0.9 5,438,044 - BWI76_RS26270 0.67 +0.5 5,438,074 + BWI76_RS26270 0.73 -0.6 5,438,182 + +0.3 5,438,212 + -0.9 5,438,227 - -0.7 5,438,248 - +1.8 5,438,256 + +0.3 5,438,264 - -0.0 5,438,264 - -0.4 5,438,264 - +0.3 5,438,309 - +0.1 5,438,309 - +0.0 5,438,337 - -0.1 5,438,763 + BWI76_RS26275 0.29 -0.3 5,438,773 + BWI76_RS26275 0.30 +0.4 5,438,773 + BWI76_RS26275 0.30 -0.8 5,439,138 - BWI76_RS26275 0.50 +3.1 5,439,140 + BWI76_RS26275 0.51 +1.5 5,439,140 + BWI76_RS26275 0.51 +0.4 5,439,148 - BWI76_RS26275 0.51 -0.1 5,439,148 - BWI76_RS26275 0.51 +0.1 5,439,212 + BWI76_RS26275 0.55 +0.7 5,439,231 + BWI76_RS26275 0.56 +0.2 5,439,267 + BWI76_RS26275 0.58 +1.6 5,439,417 - BWI76_RS26275 0.66 -2.7 5,439,503 + BWI76_RS26275 0.71 -1.5 5,439,540 - BWI76_RS26275 0.74 -1.5 5,439,572 - BWI76_RS26275 0.75 +0.4 5,439,678 + BWI76_RS26275 0.81 +0.1 5,439,686 - BWI76_RS26275 0.82 -0.2 5,439,742 + BWI76_RS26275 0.85 -1.0 5,439,854 + +0.3 5,439,897 + +0.4 5,439,897 + +0.1 5,439,905 - +0.0 5,439,942 - +2.0 5,439,963 + -0.5 5,440,096 - +1.5 5,440,112 + +0.0 5,440,115 - -0.7 5,440,178 + BWI76_RS26280 0.17 -0.3 5,440,197 - BWI76_RS26280 0.23 -0.5 5,440,218 - BWI76_RS26280 0.30 +0.3 5,440,293 + BWI76_RS26280 0.53 -1.6 5,440,293 + BWI76_RS26280 0.53 +0.4 5,440,601 - BWI76_RS26285 0.18 -0.6 5,440,635 - BWI76_RS26285 0.23 -0.3 5,440,635 - BWI76_RS26285 0.23 -1.3 5,440,698 + BWI76_RS26285 0.31 -2.4 5,440,943 - BWI76_RS26285 0.64 -1.1
Or see this region's nucleotide sequence