Experiment: Casamino Acids (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12400 and BWI76_RS12405 are separated by 509 nucleotides BWI76_RS12405 and BWI76_RS12410 are separated by 227 nucleotides BWI76_RS12410 and BWI76_RS12415 are separated by 204 nucleotides
BWI76_RS12400: BWI76_RS12400 - TetR family transcriptional regulator, at 2,589,961 to 2,590,617
_RS12400
BWI76_RS12405: BWI76_RS12405 - diguanylate phosphodiesterase, at 2,591,127 to 2,592,299
_RS12405
BWI76_RS12410: BWI76_RS12410 - hypothetical protein, at 2,592,527 to 2,592,838
_RS12410
BWI76_RS12415: BWI76_RS12415 - antimicrobial peptide ABC transporter permease SapB, at 2,593,043 to 2,594,008
_RS12415
Position (kb)
2591
2592
2593 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2590.201 kb on - strand, within BWI76_RS12400 at 2590.238 kb on - strand, within BWI76_RS12400 at 2590.340 kb on - strand, within BWI76_RS12400 at 2590.353 kb on - strand, within BWI76_RS12400 at 2590.362 kb on - strand at 2590.407 kb on - strand, within BWI76_RS12400 at 2590.524 kb on + strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.613 kb on + strand at 2590.621 kb on - strand at 2590.621 kb on - strand at 2590.679 kb on - strand at 2590.743 kb on + strand at 2590.751 kb on - strand at 2590.751 kb on - strand at 2590.751 kb on - strand at 2590.751 kb on - strand at 2590.757 kb on + strand at 2590.765 kb on - strand at 2590.775 kb on - strand at 2590.775 kb on - strand at 2590.775 kb on - strand at 2590.775 kb on - strand at 2590.779 kb on - strand at 2590.781 kb on - strand at 2590.791 kb on - strand at 2590.801 kb on + strand at 2590.814 kb on + strand at 2590.822 kb on - strand at 2590.822 kb on - strand at 2590.822 kb on - strand at 2590.822 kb on - strand at 2590.822 kb on - strand at 2590.824 kb on + strand at 2590.835 kb on + strand at 2590.859 kb on + strand at 2590.931 kb on + strand at 2590.939 kb on + strand at 2590.939 kb on + strand at 2590.940 kb on - strand at 2590.940 kb on - strand at 2590.948 kb on + strand at 2590.956 kb on - strand at 2590.961 kb on - strand at 2590.977 kb on + strand at 2591.012 kb on + strand at 2591.015 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.023 kb on + strand at 2591.031 kb on - strand at 2591.031 kb on - strand at 2591.031 kb on - strand at 2591.031 kb on - strand at 2591.031 kb on - strand at 2591.031 kb on - strand at 2591.031 kb on - strand at 2591.037 kb on + strand at 2591.037 kb on + strand at 2591.037 kb on + strand at 2591.044 kb on - strand at 2591.079 kb on + strand at 2591.159 kb on - strand at 2591.179 kb on - strand at 2591.216 kb on - strand at 2591.229 kb on - strand at 2591.235 kb on - strand at 2591.277 kb on + strand, within BWI76_RS12405 at 2591.285 kb on - strand, within BWI76_RS12405 at 2591.312 kb on - strand at 2591.400 kb on + strand, within BWI76_RS12405 at 2591.400 kb on + strand, within BWI76_RS12405 at 2591.400 kb on + strand, within BWI76_RS12405 at 2591.400 kb on + strand, within BWI76_RS12405 at 2591.623 kb on + strand at 2591.728 kb on + strand, within BWI76_RS12405 at 2591.906 kb on - strand, within BWI76_RS12405 at 2591.906 kb on - strand, within BWI76_RS12405 at 2591.954 kb on + strand, within BWI76_RS12405 at 2591.957 kb on - strand, within BWI76_RS12405 at 2591.962 kb on - strand at 2591.962 kb on - strand, within BWI76_RS12405 at 2591.975 kb on - strand, within BWI76_RS12405 at 2591.988 kb on + strand at 2591.990 kb on + strand, within BWI76_RS12405 at 2592.042 kb on - strand, within BWI76_RS12405 at 2592.045 kb on + strand, within BWI76_RS12405 at 2592.053 kb on - strand, within BWI76_RS12405 at 2592.132 kb on + strand, within BWI76_RS12405 at 2592.134 kb on + strand, within BWI76_RS12405 at 2592.135 kb on - strand, within BWI76_RS12405 at 2592.266 kb on + strand at 2592.269 kb on - strand at 2592.269 kb on - strand at 2592.497 kb on + strand at 2592.530 kb on + strand at 2592.535 kb on + strand at 2592.566 kb on + strand, within BWI76_RS12410 at 2592.589 kb on - strand, within BWI76_RS12410 at 2592.627 kb on + strand, within BWI76_RS12410 at 2592.627 kb on + strand, within BWI76_RS12410 at 2592.641 kb on + strand, within BWI76_RS12410 at 2592.641 kb on + strand, within BWI76_RS12410 at 2592.641 kb on + strand, within BWI76_RS12410 at 2592.648 kb on - strand, within BWI76_RS12410 at 2592.649 kb on - strand, within BWI76_RS12410 at 2592.656 kb on - strand, within BWI76_RS12410 at 2592.672 kb on + strand, within BWI76_RS12410 at 2592.672 kb on + strand, within BWI76_RS12410 at 2592.726 kb on - strand, within BWI76_RS12410 at 2592.794 kb on - strand, within BWI76_RS12410 at 2592.794 kb on - strand, within BWI76_RS12410 at 2592.828 kb on + strand at 2592.851 kb on - strand at 2592.873 kb on - strand at 2592.874 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino Acids (N) remove 2,590,201 - BWI76_RS12400 0.37 -1.2 2,590,238 - BWI76_RS12400 0.42 -0.7 2,590,340 - BWI76_RS12400 0.58 -2.0 2,590,353 - BWI76_RS12400 0.60 -1.8 2,590,362 - +0.5 2,590,407 - BWI76_RS12400 0.68 -0.4 2,590,524 + BWI76_RS12400 0.86 -0.2 2,590,532 - BWI76_RS12400 0.87 +0.1 2,590,532 - BWI76_RS12400 0.87 +0.4 2,590,532 - BWI76_RS12400 0.87 -1.0 2,590,532 - BWI76_RS12400 0.87 -0.2 2,590,532 - BWI76_RS12400 0.87 +1.9 2,590,613 + -1.7 2,590,621 - +2.1 2,590,621 - -1.3 2,590,679 - -1.2 2,590,743 + +0.5 2,590,751 - -1.2 2,590,751 - +1.4 2,590,751 - +0.8 2,590,751 - +1.6 2,590,757 + -2.1 2,590,765 - +0.9 2,590,775 - -1.7 2,590,775 - -0.8 2,590,775 - -1.2 2,590,775 - -0.8 2,590,779 - -1.0 2,590,781 - +0.1 2,590,791 - +1.0 2,590,801 + -2.2 2,590,814 + -1.9 2,590,822 - -2.2 2,590,822 - -2.8 2,590,822 - +0.5 2,590,822 - -1.0 2,590,822 - -1.6 2,590,824 + -0.9 2,590,835 + -1.5 2,590,859 + -0.3 2,590,931 + -0.5 2,590,939 + +1.1 2,590,939 + -1.2 2,590,940 - +0.8 2,590,940 - -0.5 2,590,948 + +0.2 2,590,956 - -0.5 2,590,961 - +2.4 2,590,977 + -2.5 2,591,012 + +0.5 2,591,015 + +0.5 2,591,023 + +0.3 2,591,023 + -1.1 2,591,023 + -1.1 2,591,023 + -0.9 2,591,023 + -1.3 2,591,023 + -1.5 2,591,023 + +0.2 2,591,023 + -1.6 2,591,023 + -2.0 2,591,023 + +0.7 2,591,023 + -0.4 2,591,023 + -0.4 2,591,023 + -2.6 2,591,031 - +0.1 2,591,031 - -1.3 2,591,031 - +1.4 2,591,031 - -2.0 2,591,031 - -0.5 2,591,031 - -0.9 2,591,031 - +0.5 2,591,037 + +1.3 2,591,037 + -0.4 2,591,037 + -0.1 2,591,044 - +1.1 2,591,079 + +2.3 2,591,159 - +0.2 2,591,179 - +2.5 2,591,216 - -0.7 2,591,229 - -1.8 2,591,235 - +0.3 2,591,277 + BWI76_RS12405 0.13 -3.1 2,591,285 - BWI76_RS12405 0.13 -0.5 2,591,312 - +3.1 2,591,400 + BWI76_RS12405 0.23 +0.1 2,591,400 + BWI76_RS12405 0.23 -0.7 2,591,400 + BWI76_RS12405 0.23 -1.2 2,591,400 + BWI76_RS12405 0.23 -1.2 2,591,623 + +1.1 2,591,728 + BWI76_RS12405 0.51 +0.4 2,591,906 - BWI76_RS12405 0.66 +0.3 2,591,906 - BWI76_RS12405 0.66 +0.1 2,591,954 + BWI76_RS12405 0.71 -1.6 2,591,957 - BWI76_RS12405 0.71 -2.7 2,591,962 - +0.5 2,591,962 - BWI76_RS12405 0.71 -0.7 2,591,975 - BWI76_RS12405 0.72 +0.1 2,591,988 + +2.1 2,591,990 + BWI76_RS12405 0.74 -2.4 2,592,042 - BWI76_RS12405 0.78 -0.9 2,592,045 + BWI76_RS12405 0.78 -1.0 2,592,053 - BWI76_RS12405 0.79 -0.8 2,592,132 + BWI76_RS12405 0.86 -0.9 2,592,134 + BWI76_RS12405 0.86 -3.3 2,592,135 - BWI76_RS12405 0.86 -1.0 2,592,266 + -1.5 2,592,269 - -0.9 2,592,269 - -0.4 2,592,497 + -0.9 2,592,530 + -0.3 2,592,535 + -0.5 2,592,566 + BWI76_RS12410 0.12 +0.9 2,592,589 - BWI76_RS12410 0.20 -2.0 2,592,627 + BWI76_RS12410 0.32 +0.2 2,592,627 + BWI76_RS12410 0.32 +0.1 2,592,641 + BWI76_RS12410 0.37 -0.2 2,592,641 + BWI76_RS12410 0.37 -0.7 2,592,641 + BWI76_RS12410 0.37 +0.6 2,592,648 - BWI76_RS12410 0.39 +1.1 2,592,649 - BWI76_RS12410 0.39 -1.2 2,592,656 - BWI76_RS12410 0.41 +2.1 2,592,672 + BWI76_RS12410 0.46 -4.0 2,592,672 + BWI76_RS12410 0.46 -0.1 2,592,726 - BWI76_RS12410 0.64 -1.6 2,592,794 - BWI76_RS12410 0.86 -1.2 2,592,794 - BWI76_RS12410 0.86 -0.6 2,592,828 + +0.1 2,592,851 - +0.6 2,592,873 - -0.9 2,592,874 - -0.3
Or see this region's nucleotide sequence