Experiment: Casamino Acids (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09170 and BWI76_RS09175 are separated by 149 nucleotides BWI76_RS09175 and BWI76_RS09180 are separated by 157 nucleotides BWI76_RS09180 and BWI76_RS09185 are separated by 50 nucleotides
BWI76_RS09170: BWI76_RS09170 - MFS transporter, at 1,937,085 to 1,938,287
_RS09170
BWI76_RS09175: BWI76_RS09175 - DNA-binding transcriptional regulator, at 1,938,437 to 1,938,979
_RS09175
BWI76_RS09180: BWI76_RS09180 - hypothetical protein, at 1,939,137 to 1,939,406
_RS09180
BWI76_RS09185: BWI76_RS09185 - integrase, at 1,939,457 to 1,940,503
_RS09185
Position (kb) 1939
1940 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1938.411 kb on - strand at 1938.524 kb on + strand, within BWI76_RS09175 at 1938.524 kb on - strand, within BWI76_RS09175 at 1938.599 kb on - strand, within BWI76_RS09175 at 1938.615 kb on + strand, within BWI76_RS09175 at 1938.727 kb on + strand, within BWI76_RS09175 at 1938.735 kb on - strand, within BWI76_RS09175 at 1938.735 kb on - strand, within BWI76_RS09175 at 1938.752 kb on - strand, within BWI76_RS09175 at 1938.837 kb on + strand, within BWI76_RS09175 at 1938.845 kb on - strand, within BWI76_RS09175 at 1938.845 kb on - strand, within BWI76_RS09175 at 1938.953 kb on + strand at 1939.094 kb on - strand at 1939.211 kb on - strand, within BWI76_RS09180 at 1939.350 kb on + strand, within BWI76_RS09180 at 1939.376 kb on + strand, within BWI76_RS09180 at 1939.376 kb on + strand, within BWI76_RS09180 at 1939.376 kb on + strand, within BWI76_RS09180 at 1939.376 kb on + strand, within BWI76_RS09180 at 1939.376 kb on + strand, within BWI76_RS09180 at 1939.384 kb on - strand at 1939.384 kb on - strand at 1939.384 kb on - strand at 1939.384 kb on - strand at 1939.466 kb on + strand at 1939.509 kb on + strand at 1939.517 kb on - strand at 1939.525 kb on - strand at 1939.525 kb on - strand at 1939.535 kb on - strand at 1939.549 kb on + strand at 1939.657 kb on + strand, within BWI76_RS09185 at 1939.695 kb on + strand, within BWI76_RS09185 at 1939.703 kb on - strand, within BWI76_RS09185 at 1939.792 kb on + strand, within BWI76_RS09185 at 1939.849 kb on + strand, within BWI76_RS09185 at 1939.850 kb on - strand, within BWI76_RS09185 at 1939.873 kb on + strand, within BWI76_RS09185 at 1940.055 kb on + strand, within BWI76_RS09185 at 1940.135 kb on - strand, within BWI76_RS09185 at 1940.168 kb on - strand, within BWI76_RS09185 at 1940.278 kb on + strand, within BWI76_RS09185 at 1940.349 kb on + strand, within BWI76_RS09185 at 1940.369 kb on - strand, within BWI76_RS09185 at 1940.384 kb on - strand, within BWI76_RS09185
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino Acids (N) remove 1,938,411 - +0.1 1,938,524 + BWI76_RS09175 0.16 +0.2 1,938,524 - BWI76_RS09175 0.16 +0.4 1,938,599 - BWI76_RS09175 0.30 +0.2 1,938,615 + BWI76_RS09175 0.33 -0.3 1,938,727 + BWI76_RS09175 0.53 +0.3 1,938,735 - BWI76_RS09175 0.55 -1.2 1,938,735 - BWI76_RS09175 0.55 -2.3 1,938,752 - BWI76_RS09175 0.58 -0.5 1,938,837 + BWI76_RS09175 0.74 -0.2 1,938,845 - BWI76_RS09175 0.75 -0.4 1,938,845 - BWI76_RS09175 0.75 -2.2 1,938,953 + +0.2 1,939,094 - +0.3 1,939,211 - BWI76_RS09180 0.27 +0.4 1,939,350 + BWI76_RS09180 0.79 -0.8 1,939,376 + BWI76_RS09180 0.89 +1.0 1,939,376 + BWI76_RS09180 0.89 +0.8 1,939,376 + BWI76_RS09180 0.89 +2.4 1,939,376 + BWI76_RS09180 0.89 -1.2 1,939,376 + BWI76_RS09180 0.89 +1.1 1,939,384 - -0.5 1,939,384 - +0.1 1,939,384 - -0.3 1,939,384 - -0.1 1,939,466 + +0.8 1,939,509 + +1.0 1,939,517 - +0.0 1,939,525 - +1.6 1,939,525 - -2.6 1,939,535 - -0.4 1,939,549 + +0.1 1,939,657 + BWI76_RS09185 0.19 -2.1 1,939,695 + BWI76_RS09185 0.23 -0.1 1,939,703 - BWI76_RS09185 0.23 +2.0 1,939,792 + BWI76_RS09185 0.32 -1.1 1,939,849 + BWI76_RS09185 0.37 -0.9 1,939,850 - BWI76_RS09185 0.38 -1.0 1,939,873 + BWI76_RS09185 0.40 -0.1 1,940,055 + BWI76_RS09185 0.57 +0.3 1,940,135 - BWI76_RS09185 0.65 -0.8 1,940,168 - BWI76_RS09185 0.68 -0.7 1,940,278 + BWI76_RS09185 0.78 -0.3 1,940,349 + BWI76_RS09185 0.85 +2.3 1,940,369 - BWI76_RS09185 0.87 -0.2 1,940,384 - BWI76_RS09185 0.89 +0.8
Or see this region's nucleotide sequence