Experiment: Casamino Acids (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07615 and BWI76_RS07620 are separated by 10 nucleotides BWI76_RS07620 and BWI76_RS07625 overlap by 84 nucleotides BWI76_RS07625 and BWI76_RS07630 overlap by 4 nucleotides BWI76_RS07630 and BWI76_RS07635 are separated by 222 nucleotides BWI76_RS07635 and BWI76_RS07640 are separated by 193 nucleotides
BWI76_RS07615: BWI76_RS07615 - betaine-aldehyde dehydrogenase, at 1,613,138 to 1,614,610
_RS07615
BWI76_RS07620: BWI76_RS07620 - transcriptional regulator BetI, at 1,614,621 to 1,615,214
_RS07620
BWI76_RS07625: BWI76_RS07625 - hypothetical protein, at 1,615,131 to 1,615,346
_RS07625
BWI76_RS07630: BWI76_RS07630 - high-affinity choline transporter BetT, at 1,615,343 to 1,617,376
_RS07630
BWI76_RS07635: BWI76_RS07635 - Hok/gef cell toxic protein, at 1,617,599 to 1,617,751
_RS07635
BWI76_RS07640: BWI76_RS07640 - short chain dehydrogenase, at 1,617,945 to 1,618,730
_RS07640
Position (kb)
1615
1616
1617
1618 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1614.458 kb on + strand, within BWI76_RS07615 at 1614.501 kb on + strand at 1614.501 kb on + strand at 1614.605 kb on + strand at 1614.702 kb on + strand, within BWI76_RS07620 at 1614.818 kb on + strand, within BWI76_RS07620 at 1615.001 kb on - strand, within BWI76_RS07620 at 1615.039 kb on + strand, within BWI76_RS07620 at 1615.467 kb on + strand at 1615.478 kb on + strand at 1615.552 kb on + strand, within BWI76_RS07630 at 1615.730 kb on - strand, within BWI76_RS07630 at 1616.014 kb on + strand at 1616.151 kb on + strand, within BWI76_RS07630 at 1616.170 kb on + strand, within BWI76_RS07630 at 1616.186 kb on - strand, within BWI76_RS07630 at 1616.421 kb on + strand, within BWI76_RS07630 at 1616.562 kb on + strand, within BWI76_RS07630 at 1616.686 kb on - strand, within BWI76_RS07630 at 1616.963 kb on + strand, within BWI76_RS07630 at 1617.084 kb on + strand, within BWI76_RS07630 at 1617.371 kb on + strand at 1617.637 kb on + strand, within BWI76_RS07635 at 1617.647 kb on - strand, within BWI76_RS07635 at 1617.647 kb on - strand, within BWI76_RS07635 at 1617.660 kb on + strand at 1617.668 kb on - strand, within BWI76_RS07635 at 1617.668 kb on - strand, within BWI76_RS07635 at 1617.800 kb on + strand at 1617.807 kb on - strand at 1617.890 kb on + strand at 1617.890 kb on - strand at 1618.235 kb on - strand, within BWI76_RS07640 at 1618.235 kb on - strand, within BWI76_RS07640 at 1618.272 kb on + strand, within BWI76_RS07640
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino Acids (N) remove 1,614,458 + BWI76_RS07615 0.90 -0.2 1,614,501 + +2.3 1,614,501 + -2.3 1,614,605 + -0.7 1,614,702 + BWI76_RS07620 0.14 +0.4 1,614,818 + BWI76_RS07620 0.33 +1.4 1,615,001 - BWI76_RS07620 0.64 -0.4 1,615,039 + BWI76_RS07620 0.70 -2.1 1,615,467 + -1.0 1,615,478 + -0.2 1,615,552 + BWI76_RS07630 0.10 +0.9 1,615,730 - BWI76_RS07630 0.19 +0.3 1,616,014 + +0.0 1,616,151 + BWI76_RS07630 0.40 -1.8 1,616,170 + BWI76_RS07630 0.41 +1.9 1,616,186 - BWI76_RS07630 0.41 +0.3 1,616,421 + BWI76_RS07630 0.53 +0.2 1,616,562 + BWI76_RS07630 0.60 -0.3 1,616,686 - BWI76_RS07630 0.66 +1.7 1,616,963 + BWI76_RS07630 0.80 +0.4 1,617,084 + BWI76_RS07630 0.86 +0.6 1,617,371 + -0.5 1,617,637 + BWI76_RS07635 0.25 -0.5 1,617,647 - BWI76_RS07635 0.31 -1.3 1,617,647 - BWI76_RS07635 0.31 -1.2 1,617,660 + +0.8 1,617,668 - BWI76_RS07635 0.45 -0.9 1,617,668 - BWI76_RS07635 0.45 -1.0 1,617,800 + +0.2 1,617,807 - +0.8 1,617,890 + +0.1 1,617,890 - -1.3 1,618,235 - BWI76_RS07640 0.37 -2.4 1,618,235 - BWI76_RS07640 0.37 +0.3 1,618,272 + BWI76_RS07640 0.42 +0.2
Or see this region's nucleotide sequence