Experiment: Casamino Acids (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00990 and BWI76_RS00995 are separated by 100 nucleotides BWI76_RS00995 and BWI76_RS01000 are separated by 52 nucleotides BWI76_RS01000 and BWI76_RS01005 are separated by 194 nucleotides BWI76_RS01005 and BWI76_RS01010 are separated by 24 nucleotides BWI76_RS01010 and BWI76_RS01015 overlap by 1 nucleotides
BWI76_RS00990: BWI76_RS00990 - ketol-acid reductoisomerase, at 202,804 to 204,279
_RS00990
BWI76_RS00995: BWI76_RS00995 - peptidyl-prolyl cis-trans isomerase, at 204,380 to 204,661
_RS00995
BWI76_RS01000: BWI76_RS01000 - peptidyl-prolyl cis-trans isomerase, at 204,714 to 204,995
_RS01000
BWI76_RS01005: BWI76_RS01005 - CarD family transcriptional regulator, at 205,190 to 205,894
_RS01005
BWI76_RS01010: BWI76_RS01010 - uridine phosphorylase, at 205,919 to 206,668
_RS01010
BWI76_RS01015: BWI76_RS01015 - nicotinamide riboside transporter PnuC, at 206,668 to 207,390
_RS01015
Position (kb) 205
206 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 204.305 kb on + strand at 204.306 kb on + strand at 204.330 kb on + strand at 204.458 kb on + strand, within BWI76_RS00995 at 204.466 kb on - strand, within BWI76_RS00995 at 204.496 kb on - strand, within BWI76_RS00995 at 204.720 kb on - strand at 204.783 kb on - strand, within BWI76_RS01000 at 204.800 kb on - strand at 204.819 kb on + strand, within BWI76_RS01000 at 204.906 kb on + strand, within BWI76_RS01000 at 204.936 kb on - strand, within BWI76_RS01000 at 204.948 kb on + strand, within BWI76_RS01000 at 204.977 kb on - strand at 204.977 kb on - strand at 205.053 kb on + strand at 205.053 kb on + strand at 205.145 kb on + strand at 205.163 kb on + strand at 205.163 kb on + strand at 205.165 kb on + strand at 205.165 kb on + strand at 205.166 kb on - strand at 205.240 kb on + strand at 205.414 kb on + strand, within BWI76_RS01005 at 205.423 kb on - strand, within BWI76_RS01005 at 205.529 kb on + strand, within BWI76_RS01005 at 205.561 kb on - strand, within BWI76_RS01005 at 205.632 kb on - strand, within BWI76_RS01005 at 205.812 kb on - strand, within BWI76_RS01005 at 205.812 kb on - strand, within BWI76_RS01005 at 205.938 kb on - strand at 206.028 kb on + strand, within BWI76_RS01010 at 206.077 kb on + strand, within BWI76_RS01010 at 206.081 kb on - strand, within BWI76_RS01010 at 206.201 kb on + strand, within BWI76_RS01010 at 206.456 kb on + strand, within BWI76_RS01010 at 206.456 kb on + strand, within BWI76_RS01010 at 206.464 kb on - strand, within BWI76_RS01010 at 206.464 kb on - strand, within BWI76_RS01010 at 206.464 kb on - strand, within BWI76_RS01010
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino Acids (N) remove 204,305 + -0.0 204,306 + -3.1 204,330 + +0.3 204,458 + BWI76_RS00995 0.28 -0.9 204,466 - BWI76_RS00995 0.30 -0.1 204,496 - BWI76_RS00995 0.41 -0.1 204,720 - -0.2 204,783 - BWI76_RS01000 0.24 -1.0 204,800 - +1.5 204,819 + BWI76_RS01000 0.37 -0.8 204,906 + BWI76_RS01000 0.68 -0.6 204,936 - BWI76_RS01000 0.79 -1.3 204,948 + BWI76_RS01000 0.83 +0.9 204,977 - +2.1 204,977 - -0.9 205,053 + +0.3 205,053 + +0.8 205,145 + -0.6 205,163 + -0.5 205,163 + +0.3 205,165 + -0.3 205,165 + +0.2 205,166 - -0.1 205,240 + +0.1 205,414 + BWI76_RS01005 0.32 -0.6 205,423 - BWI76_RS01005 0.33 -0.3 205,529 + BWI76_RS01005 0.48 +5.0 205,561 - BWI76_RS01005 0.53 +1.3 205,632 - BWI76_RS01005 0.63 +1.3 205,812 - BWI76_RS01005 0.88 +1.4 205,812 - BWI76_RS01005 0.88 -0.1 205,938 - +2.1 206,028 + BWI76_RS01010 0.15 -0.1 206,077 + BWI76_RS01010 0.21 +0.4 206,081 - BWI76_RS01010 0.22 +3.7 206,201 + BWI76_RS01010 0.38 -0.3 206,456 + BWI76_RS01010 0.72 -0.4 206,456 + BWI76_RS01010 0.72 -0.8 206,464 - BWI76_RS01010 0.73 -0.3 206,464 - BWI76_RS01010 0.73 -0.1 206,464 - BWI76_RS01010 0.73 +1.5
Or see this region's nucleotide sequence