Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25240

Experiment: Gly-DL-Asp (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25235 and BWI76_RS25240 are separated by 14 nucleotidesBWI76_RS25240 and BWI76_RS25245 are separated by 14 nucleotidesBWI76_RS25245 and BWI76_RS25250 overlap by 4 nucleotides BWI76_RS25235: BWI76_RS25235 - calcium/sodium antiporter, at 5,247,422 to 5,248,399 _RS25235 BWI76_RS25240: BWI76_RS25240 - D-arabinose 5-phosphate isomerase, at 5,248,414 to 5,249,400 _RS25240 BWI76_RS25245: BWI76_RS25245 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 5,249,415 to 5,249,981 _RS25245 BWI76_RS25250: BWI76_RS25250 - LPS export ABC transporter periplasmic protein LptC, at 5,249,978 to 5,250,553 _RS25250 Position (kb) 5248 5249 5250Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 5247.432 kb on - strandat 5247.487 kb on + strandat 5247.487 kb on + strandat 5247.487 kb on + strandat 5247.487 kb on + strandat 5247.553 kb on + strand, within BWI76_RS25235at 5247.605 kb on - strand, within BWI76_RS25235at 5247.633 kb on + strand, within BWI76_RS25235at 5247.699 kb on - strand, within BWI76_RS25235at 5247.883 kb on - strand, within BWI76_RS25235at 5247.883 kb on - strandat 5247.998 kb on + strand, within BWI76_RS25235at 5248.166 kb on + strand, within BWI76_RS25235at 5248.283 kb on + strand, within BWI76_RS25235at 5248.369 kb on + strandat 5248.369 kb on + strandat 5248.377 kb on - strandat 5248.409 kb on + strandat 5248.489 kb on + strandat 5248.489 kb on + strandat 5248.694 kb on - strand, within BWI76_RS25240at 5248.730 kb on - strand, within BWI76_RS25240at 5248.858 kb on - strand, within BWI76_RS25240at 5248.882 kb on + strand, within BWI76_RS25240at 5248.907 kb on - strand, within BWI76_RS25240at 5248.917 kb on + strand, within BWI76_RS25240at 5249.215 kb on - strand, within BWI76_RS25240at 5249.395 kb on + strandat 5249.446 kb on + strandat 5249.595 kb on + strand, within BWI76_RS25245at 5249.720 kb on + strand, within BWI76_RS25245at 5249.823 kb on + strand, within BWI76_RS25245at 5249.945 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Gly-DL-Asp (N)
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5,247,432 - +0.4
5,247,487 + -1.6
5,247,487 + +0.6
5,247,487 + -0.9
5,247,487 + -0.3
5,247,553 + BWI76_RS25235 0.13 +1.3
5,247,605 - BWI76_RS25235 0.19 +1.0
5,247,633 + BWI76_RS25235 0.22 -0.1
5,247,699 - BWI76_RS25235 0.28 +0.5
5,247,883 - BWI76_RS25235 0.47 -0.9
5,247,883 - -0.6
5,247,998 + BWI76_RS25235 0.59 +0.1
5,248,166 + BWI76_RS25235 0.76 -0.7
5,248,283 + BWI76_RS25235 0.88 -1.2
5,248,369 + +0.1
5,248,369 + +0.6
5,248,377 - +0.6
5,248,409 + -0.1
5,248,489 + -1.2
5,248,489 + +0.6
5,248,694 - BWI76_RS25240 0.28 +1.0
5,248,730 - BWI76_RS25240 0.32 +0.3
5,248,858 - BWI76_RS25240 0.45 +2.9
5,248,882 + BWI76_RS25240 0.47 +0.3
5,248,907 - BWI76_RS25240 0.50 -3.3
5,248,917 + BWI76_RS25240 0.51 -0.2
5,249,215 - BWI76_RS25240 0.81 +1.3
5,249,395 + -0.9
5,249,446 + +0.4
5,249,595 + BWI76_RS25245 0.32 +0.6
5,249,720 + BWI76_RS25245 0.54 +1.4
5,249,823 + BWI76_RS25245 0.72 +2.6
5,249,945 + +0.6

Or see this region's nucleotide sequence