Experiment: Gly-DL-Asp (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20110 and BWI76_RS20115 are separated by 30 nucleotides BWI76_RS20115 and BWI76_RS20120 are separated by 62 nucleotides BWI76_RS20120 and BWI76_RS20125 are separated by 425 nucleotides
BWI76_RS20110: BWI76_RS20110 - fimbrial assembly protein, at 4,211,975 to 4,214,524
_RS20110
BWI76_RS20115: BWI76_RS20115 - hypothetical protein, at 4,214,555 to 4,215,217
_RS20115
BWI76_RS20120: BWI76_RS20120 - fimbrial protein, at 4,215,280 to 4,215,807
_RS20120
BWI76_RS20125: BWI76_RS20125 - integrase, at 4,216,233 to 4,216,784
_RS20125
Position (kb)
4215
4216 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4214.334 kb on + strand at 4214.445 kb on + strand at 4214.453 kb on - strand at 4214.536 kb on - strand at 4214.545 kb on - strand at 4214.550 kb on - strand at 4214.573 kb on - strand at 4214.628 kb on - strand, within BWI76_RS20115 at 4214.880 kb on + strand, within BWI76_RS20115 at 4214.880 kb on + strand, within BWI76_RS20115 at 4214.888 kb on - strand at 4215.066 kb on - strand, within BWI76_RS20115 at 4215.070 kb on + strand, within BWI76_RS20115 at 4215.402 kb on + strand, within BWI76_RS20120 at 4215.414 kb on - strand, within BWI76_RS20120 at 4215.479 kb on + strand, within BWI76_RS20120 at 4215.487 kb on - strand, within BWI76_RS20120 at 4215.668 kb on + strand, within BWI76_RS20120 at 4215.676 kb on - strand, within BWI76_RS20120 at 4215.730 kb on - strand, within BWI76_RS20120 at 4215.730 kb on - strand, within BWI76_RS20120 at 4215.734 kb on + strand, within BWI76_RS20120 at 4215.745 kb on - strand, within BWI76_RS20120 at 4215.745 kb on - strand, within BWI76_RS20120 at 4215.745 kb on - strand, within BWI76_RS20120 at 4215.747 kb on + strand, within BWI76_RS20120 at 4215.747 kb on + strand, within BWI76_RS20120 at 4215.747 kb on + strand, within BWI76_RS20120 at 4215.752 kb on + strand, within BWI76_RS20120 at 4215.752 kb on + strand, within BWI76_RS20120 at 4215.752 kb on + strand, within BWI76_RS20120 at 4215.752 kb on + strand, within BWI76_RS20120 at 4215.752 kb on + strand, within BWI76_RS20120 at 4215.752 kb on + strand, within BWI76_RS20120 at 4215.752 kb on + strand, within BWI76_RS20120 at 4215.760 kb on - strand at 4215.760 kb on - strand at 4215.760 kb on - strand at 4215.760 kb on - strand at 4215.760 kb on - strand at 4215.760 kb on - strand at 4215.760 kb on - strand at 4215.760 kb on - strand at 4215.760 kb on - strand at 4215.760 kb on - strand at 4215.772 kb on + strand at 4215.780 kb on - strand at 4215.780 kb on - strand at 4215.780 kb on - strand at 4215.780 kb on - strand at 4215.780 kb on - strand at 4215.780 kb on - strand at 4215.780 kb on - strand at 4215.780 kb on - strand at 4215.788 kb on - strand at 4215.818 kb on + strand at 4215.818 kb on + strand at 4215.826 kb on - strand at 4215.848 kb on + strand at 4215.848 kb on + strand at 4215.848 kb on + strand at 4215.848 kb on + strand at 4215.848 kb on + strand at 4215.856 kb on - strand at 4215.856 kb on - strand at 4215.856 kb on - strand at 4215.856 kb on - strand at 4215.856 kb on - strand at 4215.856 kb on - strand at 4215.856 kb on - strand at 4215.885 kb on + strand at 4215.952 kb on + strand at 4216.028 kb on - strand at 4216.073 kb on - strand at 4216.074 kb on - strand at 4216.336 kb on + strand, within BWI76_RS20125 at 4216.357 kb on - strand, within BWI76_RS20125 at 4216.449 kb on + strand, within BWI76_RS20125 at 4216.529 kb on + strand, within BWI76_RS20125 at 4216.538 kb on + strand, within BWI76_RS20125 at 4216.539 kb on - strand, within BWI76_RS20125 at 4216.545 kb on - strand, within BWI76_RS20125 at 4216.546 kb on - strand, within BWI76_RS20125 at 4216.666 kb on - strand, within BWI76_RS20125 at 4216.719 kb on + strand, within BWI76_RS20125 at 4216.719 kb on + strand, within BWI76_RS20125 at 4216.723 kb on - strand, within BWI76_RS20125 at 4216.727 kb on - strand, within BWI76_RS20125 at 4216.728 kb on - strand, within BWI76_RS20125 at 4216.740 kb on + strand at 4216.741 kb on + strand at 4216.798 kb on + strand at 4216.798 kb on + strand at 4216.806 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (N) remove 4,214,334 + +0.8 4,214,445 + +1.2 4,214,453 - -0.8 4,214,536 - -2.2 4,214,545 - -2.5 4,214,550 - +0.8 4,214,573 - -0.8 4,214,628 - BWI76_RS20115 0.11 +0.2 4,214,880 + BWI76_RS20115 0.49 +0.2 4,214,880 + BWI76_RS20115 0.49 +1.2 4,214,888 - +0.2 4,215,066 - BWI76_RS20115 0.77 +0.1 4,215,070 + BWI76_RS20115 0.78 -1.6 4,215,402 + BWI76_RS20120 0.23 +0.7 4,215,414 - BWI76_RS20120 0.25 -0.5 4,215,479 + BWI76_RS20120 0.38 +0.3 4,215,487 - BWI76_RS20120 0.39 -0.4 4,215,668 + BWI76_RS20120 0.73 +0.6 4,215,676 - BWI76_RS20120 0.75 -1.9 4,215,730 - BWI76_RS20120 0.85 -2.1 4,215,730 - BWI76_RS20120 0.85 -1.3 4,215,734 + BWI76_RS20120 0.86 -2.0 4,215,745 - BWI76_RS20120 0.88 -0.5 4,215,745 - BWI76_RS20120 0.88 +0.5 4,215,745 - BWI76_RS20120 0.88 -0.4 4,215,747 + BWI76_RS20120 0.88 -1.6 4,215,747 + BWI76_RS20120 0.88 -1.4 4,215,747 + BWI76_RS20120 0.88 -1.1 4,215,752 + BWI76_RS20120 0.89 -0.9 4,215,752 + BWI76_RS20120 0.89 +0.2 4,215,752 + BWI76_RS20120 0.89 -0.4 4,215,752 + BWI76_RS20120 0.89 -1.3 4,215,752 + BWI76_RS20120 0.89 +1.2 4,215,752 + BWI76_RS20120 0.89 +0.0 4,215,752 + BWI76_RS20120 0.89 -1.4 4,215,760 - -0.4 4,215,760 - +1.6 4,215,760 - -0.0 4,215,760 - +0.2 4,215,760 - -3.0 4,215,760 - +0.4 4,215,760 - +1.8 4,215,760 - +1.8 4,215,760 - +2.1 4,215,760 - -0.5 4,215,772 + -0.5 4,215,780 - +1.1 4,215,780 - +0.0 4,215,780 - -0.2 4,215,780 - +0.6 4,215,780 - -2.5 4,215,780 - +0.6 4,215,780 - +0.4 4,215,780 - -1.8 4,215,788 - -0.7 4,215,818 + -2.6 4,215,818 + -0.8 4,215,826 - +2.1 4,215,848 + -1.1 4,215,848 + +0.5 4,215,848 + -1.1 4,215,848 + -0.9 4,215,848 + +0.2 4,215,856 - -1.7 4,215,856 - +0.9 4,215,856 - +1.0 4,215,856 - +0.4 4,215,856 - -1.5 4,215,856 - -0.8 4,215,856 - -0.7 4,215,885 + -0.5 4,215,952 + +2.1 4,216,028 - +0.8 4,216,073 - +0.5 4,216,074 - +0.8 4,216,336 + BWI76_RS20125 0.19 -0.1 4,216,357 - BWI76_RS20125 0.22 -0.2 4,216,449 + BWI76_RS20125 0.39 +1.1 4,216,529 + BWI76_RS20125 0.54 -0.1 4,216,538 + BWI76_RS20125 0.55 -0.1 4,216,539 - BWI76_RS20125 0.55 -1.9 4,216,545 - BWI76_RS20125 0.57 +2.2 4,216,546 - BWI76_RS20125 0.57 +2.7 4,216,666 - BWI76_RS20125 0.78 -0.7 4,216,719 + BWI76_RS20125 0.88 +0.6 4,216,719 + BWI76_RS20125 0.88 -1.3 4,216,723 - BWI76_RS20125 0.89 +0.0 4,216,727 - BWI76_RS20125 0.89 -2.4 4,216,728 - BWI76_RS20125 0.90 -0.9 4,216,740 + -0.9 4,216,741 + +0.4 4,216,798 + -1.5 4,216,798 + +0.8 4,216,806 - +1.3
Or see this region's nucleotide sequence