Experiment: Gly-DL-Asp (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16850 and BWI76_RS16855 overlap by 4 nucleotides BWI76_RS16855 and BWI76_RS16860 are separated by 11 nucleotides BWI76_RS16860 and BWI76_RS16865 are separated by 53 nucleotides
BWI76_RS16850: BWI76_RS16850 - ABC transporter periplasmic substrate-binding protein, at 3,494,332 to 3,495,369
_RS16850
BWI76_RS16855: BWI76_RS16855 - putative ABC transporter periplasmic solute-binding protein, at 3,495,366 to 3,496,340
_RS16855
BWI76_RS16860: BWI76_RS16860 - acyl-CoA dehydrogenase, at 3,496,352 to 3,497,440
_RS16860
BWI76_RS16865: BWI76_RS16865 - coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase-related flavin-dependent oxidoreductase, at 3,497,494 to 3,498,579
_RS16865
Position (kb)
3496
3497
3498 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3495.367 kb on - strand at 3495.387 kb on + strand at 3495.787 kb on + strand, within BWI76_RS16855 at 3496.282 kb on - strand at 3496.429 kb on - strand at 3496.666 kb on + strand, within BWI76_RS16860 at 3496.720 kb on - strand, within BWI76_RS16860 at 3497.655 kb on + strand, within BWI76_RS16865 at 3497.663 kb on + strand, within BWI76_RS16865 at 3497.663 kb on - strand, within BWI76_RS16865 at 3497.738 kb on + strand, within BWI76_RS16865 at 3498.029 kb on + strand, within BWI76_RS16865 at 3498.154 kb on + strand, within BWI76_RS16865 at 3498.173 kb on + strand at 3498.181 kb on - strand, within BWI76_RS16865
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (N) remove 3,495,367 - -0.0 3,495,387 + +0.5 3,495,787 + BWI76_RS16855 0.43 -0.8 3,496,282 - -0.9 3,496,429 - -3.2 3,496,666 + BWI76_RS16860 0.29 -0.7 3,496,720 - BWI76_RS16860 0.34 -1.0 3,497,655 + BWI76_RS16865 0.15 +0.2 3,497,663 + BWI76_RS16865 0.16 +1.1 3,497,663 - BWI76_RS16865 0.16 -0.5 3,497,738 + BWI76_RS16865 0.22 -1.0 3,498,029 + BWI76_RS16865 0.49 +1.0 3,498,154 + BWI76_RS16865 0.61 -0.4 3,498,173 + +1.8 3,498,181 - BWI76_RS16865 0.63 +2.6
Or see this region's nucleotide sequence