Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15220

Experiment: Gly-DL-Asp (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15210 and BWI76_RS15215 overlap by 47 nucleotidesBWI76_RS15215 and BWI76_RS15220 are separated by 145 nucleotidesBWI76_RS15220 and BWI76_RS15225 are separated by 12 nucleotidesBWI76_RS15225 and BWI76_RS15230 are separated by 1 nucleotides BWI76_RS15210: BWI76_RS15210 - LysR family transcriptional regulator, at 3,188,634 to 3,189,512 _RS15210 BWI76_RS15215: BWI76_RS15215 - N-acetyltransferase, at 3,189,466 to 3,189,948 _RS15215 BWI76_RS15220: BWI76_RS15220 - L-lactate dehydrogenase, at 3,190,094 to 3,191,038 _RS15220 BWI76_RS15225: BWI76_RS15225 - DeoR family transcriptional regulator, at 3,191,051 to 3,191,812 _RS15225 BWI76_RS15230: BWI76_RS15230 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 3,191,814 to 3,192,800 _RS15230 Position (kb) 3190 3191 3192Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3189.178 kb on + strand, within BWI76_RS15210at 3189.322 kb on + strand, within BWI76_RS15210at 3189.351 kb on - strand, within BWI76_RS15210at 3189.595 kb on + strand, within BWI76_RS15215at 3190.233 kb on + strand, within BWI76_RS15220at 3190.611 kb on + strand, within BWI76_RS15220at 3190.619 kb on - strand, within BWI76_RS15220at 3191.144 kb on + strand, within BWI76_RS15225at 3191.152 kb on - strand, within BWI76_RS15225at 3191.276 kb on - strand, within BWI76_RS15225at 3191.685 kb on + strand, within BWI76_RS15225at 3191.693 kb on - strand, within BWI76_RS15225at 3191.848 kb on - strandat 3191.851 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Gly-DL-Asp (N)
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3,189,178 + BWI76_RS15210 0.62 +0.1
3,189,322 + BWI76_RS15210 0.78 +1.1
3,189,351 - BWI76_RS15210 0.82 +3.2
3,189,595 + BWI76_RS15215 0.27 -3.5
3,190,233 + BWI76_RS15220 0.15 -0.4
3,190,611 + BWI76_RS15220 0.55 -0.3
3,190,619 - BWI76_RS15220 0.56 -0.4
3,191,144 + BWI76_RS15225 0.12 +1.4
3,191,152 - BWI76_RS15225 0.13 -0.5
3,191,276 - BWI76_RS15225 0.30 -0.5
3,191,685 + BWI76_RS15225 0.83 +1.1
3,191,693 - BWI76_RS15225 0.84 +0.6
3,191,848 - -0.2
3,191,851 - +1.6

Or see this region's nucleotide sequence