Experiment: Gly-DL-Asp (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11055 and BWI76_RS11060 are separated by 190 nucleotides BWI76_RS11060 and BWI76_RS11065 are separated by 141 nucleotides BWI76_RS11065 and BWI76_RS11070 are separated by 16 nucleotides BWI76_RS11070 and BWI76_RS11075 are separated by 79 nucleotides
BWI76_RS11055: BWI76_RS11055 - hydrolase, at 2,333,421 to 2,334,566
_RS11055
BWI76_RS11060: BWI76_RS11060 - septum formation inhibitor Maf, at 2,334,757 to 2,335,341
_RS11060
BWI76_RS11065: BWI76_RS11065 - hypothetical protein, at 2,335,483 to 2,336,004
_RS11065
BWI76_RS11070: BWI76_RS11070 - 50S ribosomal protein L32, at 2,336,021 to 2,336,194
_RS11070
BWI76_RS11075: BWI76_RS11075 - phosphate acyltransferase, at 2,336,274 to 2,337,371
_RS11075
Position (kb)
2334
2335
2336 Strain fitness (log2 ratio)
-1
0
1
2 at 2333.838 kb on + strand, within BWI76_RS11055 at 2334.197 kb on + strand, within BWI76_RS11055 at 2334.286 kb on - strand, within BWI76_RS11055 at 2334.373 kb on - strand, within BWI76_RS11055 at 2334.548 kb on - strand at 2334.685 kb on + strand at 2334.914 kb on - strand, within BWI76_RS11060 at 2335.003 kb on - strand at 2335.153 kb on + strand, within BWI76_RS11060
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (N) remove 2,333,838 + BWI76_RS11055 0.36 +0.3 2,334,197 + BWI76_RS11055 0.68 -0.7 2,334,286 - BWI76_RS11055 0.75 +0.9 2,334,373 - BWI76_RS11055 0.83 -1.0 2,334,548 - -0.3 2,334,685 + -0.6 2,334,914 - BWI76_RS11060 0.27 +0.5 2,335,003 - +0.4 2,335,153 + BWI76_RS11060 0.68 +2.3
Or see this region's nucleotide sequence