Experiment: Gly-DL-Asp (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10500 and BWI76_RS10505 are separated by 346 nucleotides BWI76_RS10505 and BWI76_RS10510 overlap by 23 nucleotides BWI76_RS10510 and BWI76_RS10515 overlap by 4 nucleotides BWI76_RS10515 and BWI76_RS10520 are separated by 41 nucleotides
BWI76_RS10500: BWI76_RS10500 - flavoprotein monooxygenase, at 2,218,667 to 2,219,821
_RS10500
BWI76_RS10505: BWI76_RS10505 - putative guanine/xanthin permease, at 2,220,168 to 2,221,550
_RS10505
BWI76_RS10510: BWI76_RS10510 - OHCU decarboxylase, at 2,221,528 to 2,222,028
_RS10510
BWI76_RS10515: BWI76_RS10515 - 5-hydroxyisourate hydrolase, at 2,222,025 to 2,222,351
_RS10515
BWI76_RS10520: BWI76_RS10520 - guanine deaminase, at 2,222,393 to 2,223,712
_RS10520
Position (kb)
2220
2221
2222 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2219.578 kb on + strand, within BWI76_RS10500 at 2219.656 kb on - strand, within BWI76_RS10500 at 2220.354 kb on - strand, within BWI76_RS10505 at 2220.938 kb on + strand, within BWI76_RS10505 at 2221.105 kb on - strand, within BWI76_RS10505 at 2221.210 kb on - strand, within BWI76_RS10505 at 2221.796 kb on + strand, within BWI76_RS10510 at 2221.814 kb on - strand, within BWI76_RS10510 at 2221.970 kb on + strand, within BWI76_RS10510 at 2222.383 kb on + strand at 2222.483 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (N) remove 2,219,578 + BWI76_RS10500 0.79 -3.1 2,219,656 - BWI76_RS10500 0.86 -0.1 2,220,354 - BWI76_RS10505 0.13 -0.5 2,220,938 + BWI76_RS10505 0.56 -1.0 2,221,105 - BWI76_RS10505 0.68 +0.3 2,221,210 - BWI76_RS10505 0.75 -0.4 2,221,796 + BWI76_RS10510 0.53 -0.7 2,221,814 - BWI76_RS10510 0.57 -0.5 2,221,970 + BWI76_RS10510 0.88 -0.3 2,222,383 + -0.3 2,222,483 + +0.7
Or see this region's nucleotide sequence