Experiment: Gly-DL-Asp (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10020 and BWI76_RS10025 overlap by 4 nucleotides BWI76_RS10025 and BWI76_RS10030 are separated by 235 nucleotides
BWI76_RS10020: BWI76_RS10020 - ABC transporter, at 2,120,205 to 2,122,352
_RS10020
BWI76_RS10025: BWI76_RS10025 - secretion protein HlyD, at 2,122,349 to 2,123,554
_RS10025
BWI76_RS10030: BWI76_RS10030 - glycosyl transferase, at 2,123,790 to 2,126,933
_RS10030
Position (kb)
2122
2123
2124 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2121.562 kb on - strand, within BWI76_RS10020 at 2121.738 kb on + strand, within BWI76_RS10020 at 2121.837 kb on - strand, within BWI76_RS10020 at 2121.847 kb on - strand, within BWI76_RS10020 at 2121.978 kb on + strand, within BWI76_RS10020 at 2121.983 kb on + strand, within BWI76_RS10020 at 2122.252 kb on + strand at 2122.261 kb on + strand at 2122.269 kb on - strand at 2122.590 kb on + strand, within BWI76_RS10025 at 2122.624 kb on - strand, within BWI76_RS10025 at 2122.624 kb on - strand, within BWI76_RS10025 at 2122.888 kb on - strand, within BWI76_RS10025 at 2122.926 kb on + strand, within BWI76_RS10025 at 2122.934 kb on - strand, within BWI76_RS10025 at 2122.941 kb on + strand, within BWI76_RS10025 at 2122.949 kb on - strand, within BWI76_RS10025 at 2123.018 kb on - strand, within BWI76_RS10025 at 2123.023 kb on + strand, within BWI76_RS10025 at 2123.046 kb on - strand, within BWI76_RS10025 at 2123.052 kb on - strand, within BWI76_RS10025 at 2123.057 kb on - strand, within BWI76_RS10025 at 2123.065 kb on - strand, within BWI76_RS10025 at 2123.090 kb on + strand, within BWI76_RS10025 at 2123.090 kb on + strand, within BWI76_RS10025 at 2123.090 kb on + strand, within BWI76_RS10025 at 2123.097 kb on - strand, within BWI76_RS10025 at 2123.111 kb on + strand, within BWI76_RS10025 at 2123.111 kb on + strand, within BWI76_RS10025 at 2123.111 kb on + strand, within BWI76_RS10025 at 2123.119 kb on - strand, within BWI76_RS10025 at 2123.119 kb on - strand, within BWI76_RS10025 at 2123.119 kb on - strand, within BWI76_RS10025 at 2123.137 kb on + strand, within BWI76_RS10025 at 2123.154 kb on + strand, within BWI76_RS10025 at 2123.164 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.177 kb on - strand, within BWI76_RS10025 at 2123.177 kb on - strand, within BWI76_RS10025 at 2123.177 kb on - strand, within BWI76_RS10025 at 2123.214 kb on - strand, within BWI76_RS10025 at 2123.256 kb on - strand, within BWI76_RS10025 at 2123.324 kb on + strand, within BWI76_RS10025 at 2123.339 kb on - strand, within BWI76_RS10025 at 2123.348 kb on + strand, within BWI76_RS10025 at 2123.348 kb on + strand, within BWI76_RS10025 at 2123.348 kb on + strand, within BWI76_RS10025 at 2123.356 kb on - strand, within BWI76_RS10025 at 2123.356 kb on - strand, within BWI76_RS10025 at 2123.356 kb on - strand, within BWI76_RS10025 at 2123.392 kb on + strand, within BWI76_RS10025 at 2123.410 kb on - strand, within BWI76_RS10025 at 2123.456 kb on - strand at 2123.473 kb on + strand at 2123.527 kb on + strand at 2123.535 kb on - strand at 2123.565 kb on + strand at 2123.570 kb on + strand at 2123.570 kb on + strand at 2123.573 kb on - strand at 2123.578 kb on - strand at 2123.578 kb on - strand at 2123.610 kb on + strand at 2123.610 kb on + strand at 2123.610 kb on + strand at 2123.610 kb on + strand at 2123.613 kb on - strand at 2123.615 kb on + strand at 2123.615 kb on + strand at 2123.618 kb on - strand at 2123.623 kb on - strand at 2123.623 kb on - strand at 2123.665 kb on - strand at 2123.681 kb on - strand at 2123.684 kb on + strand at 2123.692 kb on - strand at 2123.694 kb on - strand at 2123.696 kb on + strand at 2123.704 kb on - strand at 2123.763 kb on - strand at 2123.789 kb on - strand at 2123.791 kb on - strand at 2123.803 kb on + strand at 2123.863 kb on - strand at 2123.864 kb on - strand at 2123.864 kb on - strand at 2123.880 kb on + strand at 2123.966 kb on + strand at 2124.176 kb on + strand, within BWI76_RS10030 at 2124.184 kb on - strand, within BWI76_RS10030 at 2124.367 kb on - strand, within BWI76_RS10030 at 2124.537 kb on - strand, within BWI76_RS10030
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (N) remove 2,121,562 - BWI76_RS10020 0.63 -2.9 2,121,738 + BWI76_RS10020 0.71 -3.0 2,121,837 - BWI76_RS10020 0.76 -1.9 2,121,847 - BWI76_RS10020 0.76 +0.4 2,121,978 + BWI76_RS10020 0.83 +0.4 2,121,983 + BWI76_RS10020 0.83 -0.7 2,122,252 + -1.4 2,122,261 + -0.4 2,122,269 - -1.5 2,122,590 + BWI76_RS10025 0.20 +0.5 2,122,624 - BWI76_RS10025 0.23 -3.3 2,122,624 - BWI76_RS10025 0.23 -0.9 2,122,888 - BWI76_RS10025 0.45 -0.4 2,122,926 + BWI76_RS10025 0.48 -1.0 2,122,934 - BWI76_RS10025 0.49 +2.1 2,122,941 + BWI76_RS10025 0.49 +0.1 2,122,949 - BWI76_RS10025 0.50 -0.2 2,123,018 - BWI76_RS10025 0.55 -0.4 2,123,023 + BWI76_RS10025 0.56 -0.4 2,123,046 - BWI76_RS10025 0.58 -1.5 2,123,052 - BWI76_RS10025 0.58 +1.8 2,123,057 - BWI76_RS10025 0.59 +1.7 2,123,065 - BWI76_RS10025 0.59 -0.9 2,123,090 + BWI76_RS10025 0.61 +1.4 2,123,090 + BWI76_RS10025 0.61 +1.0 2,123,090 + BWI76_RS10025 0.61 -0.0 2,123,097 - BWI76_RS10025 0.62 -0.7 2,123,111 + BWI76_RS10025 0.63 -0.3 2,123,111 + BWI76_RS10025 0.63 -0.7 2,123,111 + BWI76_RS10025 0.63 -0.2 2,123,119 - BWI76_RS10025 0.64 +0.8 2,123,119 - BWI76_RS10025 0.64 -1.0 2,123,119 - BWI76_RS10025 0.64 +2.6 2,123,137 + BWI76_RS10025 0.65 -0.7 2,123,154 + BWI76_RS10025 0.67 +0.6 2,123,164 + BWI76_RS10025 0.68 +0.5 2,123,169 + BWI76_RS10025 0.68 +1.6 2,123,169 + BWI76_RS10025 0.68 +0.6 2,123,169 + BWI76_RS10025 0.68 +0.5 2,123,169 + BWI76_RS10025 0.68 -0.7 2,123,169 + BWI76_RS10025 0.68 -0.3 2,123,177 - BWI76_RS10025 0.69 +0.2 2,123,177 - BWI76_RS10025 0.69 -0.1 2,123,177 - BWI76_RS10025 0.69 -0.2 2,123,214 - BWI76_RS10025 0.72 -1.5 2,123,256 - BWI76_RS10025 0.75 +0.6 2,123,324 + BWI76_RS10025 0.81 -1.2 2,123,339 - BWI76_RS10025 0.82 -1.7 2,123,348 + BWI76_RS10025 0.83 -1.8 2,123,348 + BWI76_RS10025 0.83 +0.1 2,123,348 + BWI76_RS10025 0.83 -0.6 2,123,356 - BWI76_RS10025 0.83 +0.2 2,123,356 - BWI76_RS10025 0.83 -2.9 2,123,356 - BWI76_RS10025 0.83 -0.7 2,123,392 + BWI76_RS10025 0.86 +0.5 2,123,410 - BWI76_RS10025 0.88 -2.3 2,123,456 - +1.2 2,123,473 + -0.0 2,123,527 + +0.8 2,123,535 - +1.2 2,123,565 + +0.5 2,123,570 + -0.7 2,123,570 + +0.4 2,123,573 - +0.6 2,123,578 - +0.8 2,123,578 - -2.7 2,123,610 + +0.4 2,123,610 + +0.3 2,123,610 + +1.2 2,123,610 + -1.4 2,123,613 - +0.2 2,123,615 + +1.6 2,123,615 + -0.1 2,123,618 - -0.1 2,123,623 - +0.1 2,123,623 - -1.8 2,123,665 - -0.4 2,123,681 - +0.6 2,123,684 + +0.8 2,123,692 - +0.0 2,123,694 - -0.7 2,123,696 + +0.4 2,123,704 - +0.4 2,123,763 - -0.9 2,123,789 - +2.8 2,123,791 - +0.3 2,123,803 + -2.3 2,123,863 - +0.6 2,123,864 - +2.8 2,123,864 - +0.0 2,123,880 + -2.5 2,123,966 + -0.2 2,124,176 + BWI76_RS10030 0.12 +2.0 2,124,184 - BWI76_RS10030 0.13 +1.2 2,124,367 - BWI76_RS10030 0.18 +1.2 2,124,537 - BWI76_RS10030 0.24 -0.2
Or see this region's nucleotide sequence