Experiment: Gly-DL-Asp (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07080 and BWI76_RS07085 are separated by 3 nucleotides BWI76_RS07085 and BWI76_RS07090 are separated by 175 nucleotides BWI76_RS07090 and BWI76_RS07095 are separated by 137 nucleotides BWI76_RS07095 and BWI76_RS07100 overlap by 1 nucleotides
BWI76_RS07080: BWI76_RS07080 - UDP-2,3-diacylglucosamine diphosphatase, at 1,488,656 to 1,489,378
_RS07080
BWI76_RS07085: BWI76_RS07085 - peptidyl-prolyl cis-trans isomerase, at 1,489,382 to 1,489,876
_RS07085
BWI76_RS07090: BWI76_RS07090 - cysteine--tRNA ligase, at 1,490,052 to 1,491,437
_RS07090
BWI76_RS07095: BWI76_RS07095 - ribosome-associated protein, at 1,491,575 to 1,491,787
_RS07095
BWI76_RS07100: BWI76_RS07100 - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, at 1,491,787 to 1,492,653
_RS07100
Position (kb)
1490
1491
1492 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1489.529 kb on - strand, within BWI76_RS07085 at 1489.987 kb on - strand at 1491.487 kb on + strand at 1491.487 kb on + strand at 1491.586 kb on + strand at 1491.609 kb on - strand, within BWI76_RS07095 at 1491.674 kb on - strand, within BWI76_RS07095 at 1491.730 kb on - strand, within BWI76_RS07095 at 1491.731 kb on - strand, within BWI76_RS07095 at 1491.803 kb on - strand at 1491.813 kb on - strand at 1491.822 kb on + strand at 1491.822 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (N) remove 1,489,529 - BWI76_RS07085 0.30 +1.4 1,489,987 - -1.5 1,491,487 + -0.1 1,491,487 + -1.8 1,491,586 + -0.2 1,491,609 - BWI76_RS07095 0.16 +0.5 1,491,674 - BWI76_RS07095 0.46 -2.0 1,491,730 - BWI76_RS07095 0.73 +0.0 1,491,731 - BWI76_RS07095 0.73 -1.5 1,491,803 - -1.8 1,491,813 - -3.3 1,491,822 + -3.7 1,491,822 + -3.1
Or see this region's nucleotide sequence