Experiment: Gly-DL-Asp (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02485 and BWI76_RS02490 are separated by 286 nucleotides BWI76_RS02490 and BWI76_RS02495 are separated by 23 nucleotides BWI76_RS02495 and BWI76_RS02500 are separated by 24 nucleotides BWI76_RS02500 and BWI76_RS02505 are separated by 270 nucleotides BWI76_RS02505 and BWI76_RS02510 overlap by 2 nucleotides
BWI76_RS02485: BWI76_RS02485 - polar amino acid ABC transporter (PAAT) family, periplasmic amino acid-binding protein, at 510,980 to 511,711
_RS02485
BWI76_RS02490: BWI76_RS02490 - tRNA-Gly, at 511,998 to 512,073
_RS02490
BWI76_RS02495: BWI76_RS02495 - tRNA-Gly, at 512,097 to 512,172
_RS02495
BWI76_RS02500: BWI76_RS02500 - tRNA-Gly, at 512,197 to 512,272
_RS02500
BWI76_RS02505: BWI76_RS02505 - tRNA epoxyqueuosine(34) reductase QueG, at 512,543 to 513,682
_RS02505
BWI76_RS02510: BWI76_RS02510 - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 513,681 to 515,207
_RS02510
Position (kb) 512
513
514 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 511.550 kb on - strand, within BWI76_RS02485 at 511.559 kb on + strand, within BWI76_RS02485 at 512.582 kb on - strand at 512.584 kb on + strand at 512.689 kb on - strand, within BWI76_RS02505 at 512.733 kb on + strand, within BWI76_RS02505 at 512.733 kb on + strand, within BWI76_RS02505 at 512.733 kb on + strand, within BWI76_RS02505 at 512.868 kb on + strand, within BWI76_RS02505 at 513.045 kb on + strand, within BWI76_RS02505 at 513.201 kb on - strand, within BWI76_RS02505 at 513.203 kb on + strand, within BWI76_RS02505 at 513.354 kb on - strand, within BWI76_RS02505 at 513.354 kb on - strand, within BWI76_RS02505 at 513.389 kb on - strand, within BWI76_RS02505 at 513.481 kb on + strand, within BWI76_RS02505 at 513.567 kb on - strand, within BWI76_RS02505 at 513.610 kb on + strand at 513.617 kb on + strand at 513.622 kb on + strand at 513.625 kb on - strand at 513.712 kb on + strand at 513.752 kb on - strand at 513.778 kb on + strand at 513.778 kb on + strand at 513.778 kb on + strand at 513.778 kb on + strand at 513.786 kb on - strand at 513.913 kb on - strand, within BWI76_RS02510 at 513.935 kb on - strand, within BWI76_RS02510 at 514.099 kb on - strand at 514.101 kb on + strand, within BWI76_RS02510 at 514.101 kb on + strand, within BWI76_RS02510 at 514.104 kb on - strand, within BWI76_RS02510 at 514.109 kb on - strand, within BWI76_RS02510 at 514.156 kb on - strand, within BWI76_RS02510 at 514.194 kb on + strand, within BWI76_RS02510 at 514.203 kb on + strand, within BWI76_RS02510 at 514.250 kb on - strand, within BWI76_RS02510 at 514.310 kb on - strand, within BWI76_RS02510 at 514.327 kb on + strand, within BWI76_RS02510 at 514.639 kb on + strand, within BWI76_RS02510 at 514.666 kb on + strand, within BWI76_RS02510
Per-strain Table
Position Strand Gene LocusTag Fraction Gly-DL-Asp (N) remove 511,550 - BWI76_RS02485 0.78 -0.9 511,559 + BWI76_RS02485 0.79 +0.5 512,582 - +1.0 512,584 + +1.4 512,689 - BWI76_RS02505 0.13 +0.7 512,733 + BWI76_RS02505 0.17 +1.3 512,733 + BWI76_RS02505 0.17 +1.7 512,733 + BWI76_RS02505 0.17 +0.3 512,868 + BWI76_RS02505 0.29 -1.6 513,045 + BWI76_RS02505 0.44 +1.4 513,201 - BWI76_RS02505 0.58 +0.3 513,203 + BWI76_RS02505 0.58 +1.0 513,354 - BWI76_RS02505 0.71 +1.0 513,354 - BWI76_RS02505 0.71 +0.2 513,389 - BWI76_RS02505 0.74 +1.1 513,481 + BWI76_RS02505 0.82 +0.2 513,567 - BWI76_RS02505 0.90 +1.5 513,610 + +1.4 513,617 + +1.2 513,622 + +3.7 513,625 - +1.8 513,712 + +1.3 513,752 - -1.1 513,778 + +0.7 513,778 + -1.9 513,778 + -0.1 513,778 + -2.2 513,786 - +0.3 513,913 - BWI76_RS02510 0.15 -0.8 513,935 - BWI76_RS02510 0.17 +0.9 514,099 - +1.9 514,101 + BWI76_RS02510 0.28 -0.9 514,101 + BWI76_RS02510 0.28 +0.1 514,104 - BWI76_RS02510 0.28 +0.2 514,109 - BWI76_RS02510 0.28 +0.5 514,156 - BWI76_RS02510 0.31 -1.1 514,194 + BWI76_RS02510 0.34 -2.4 514,203 + BWI76_RS02510 0.34 +0.2 514,250 - BWI76_RS02510 0.37 +0.6 514,310 - BWI76_RS02510 0.41 +2.0 514,327 + BWI76_RS02510 0.42 -0.7 514,639 + BWI76_RS02510 0.63 -0.4 514,666 + BWI76_RS02510 0.65 -1.8
Or see this region's nucleotide sequence