Experiment: reconstituted MRS with Difucosyllactose 7.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13345 and BBR_RS20760 are separated by 124 nucleotides BBR_RS20760 and BBR_RS20765 are separated by 138 nucleotides BBR_RS20765 and BBR_RS20770 are separated by 126 nucleotides BBR_RS20770 and BBR_RS13355 are separated by 229 nucleotides
BBR_RS13345: BBR_RS13345 - permease, at 764,928 to 766,073
_RS13345
BBR_RS20760: BBR_RS20760 - hypothetical protein, at 766,198 to 766,272
_RS20760
BBR_RS20765: BBR_RS20765 - 50S ribosomal protein L31, at 766,411 to 766,635
_RS20765
BBR_RS20770: BBR_RS20770 - 30S ribosomal protein S14, at 766,762 to 766,934
_RS20770
BBR_RS13355: BBR_RS13355 - ABC transporter substrate-binding protein, at 767,164 to 768,087
_RS13355
Position (kb) 766
767 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 765.204 kb on - strand, within BBR_RS13345 at 765.232 kb on + strand, within BBR_RS13345 at 765.262 kb on - strand, within BBR_RS13345 at 765.269 kb on - strand, within BBR_RS13345 at 765.306 kb on + strand, within BBR_RS13345 at 765.349 kb on - strand, within BBR_RS13345 at 765.418 kb on + strand, within BBR_RS13345 at 765.425 kb on + strand, within BBR_RS13345 at 765.425 kb on + strand, within BBR_RS13345 at 765.433 kb on - strand, within BBR_RS13345 at 765.463 kb on - strand, within BBR_RS13345 at 765.482 kb on + strand, within BBR_RS13345 at 765.482 kb on + strand, within BBR_RS13345 at 765.488 kb on - strand, within BBR_RS13345 at 765.490 kb on - strand, within BBR_RS13345 at 765.490 kb on - strand, within BBR_RS13345 at 765.490 kb on - strand, within BBR_RS13345 at 765.492 kb on - strand, within BBR_RS13345 at 765.495 kb on - strand, within BBR_RS13345 at 765.543 kb on + strand, within BBR_RS13345 at 765.551 kb on - strand, within BBR_RS13345 at 765.551 kb on - strand, within BBR_RS13345 at 765.551 kb on - strand, within BBR_RS13345 at 765.551 kb on - strand, within BBR_RS13345 at 765.551 kb on - strand, within BBR_RS13345 at 765.551 kb on - strand, within BBR_RS13345 at 765.624 kb on + strand, within BBR_RS13345 at 765.632 kb on - strand, within BBR_RS13345 at 765.665 kb on - strand, within BBR_RS13345 at 765.671 kb on - strand, within BBR_RS13345 at 765.687 kb on + strand, within BBR_RS13345 at 765.699 kb on - strand, within BBR_RS13345 at 765.721 kb on + strand, within BBR_RS13345 at 765.727 kb on - strand, within BBR_RS13345 at 765.740 kb on + strand, within BBR_RS13345 at 765.810 kb on - strand, within BBR_RS13345 at 765.866 kb on + strand, within BBR_RS13345 at 765.894 kb on - strand, within BBR_RS13345 at 765.988 kb on - strand at 766.010 kb on - strand at 766.010 kb on - strand at 766.020 kb on + strand at 766.023 kb on - strand at 766.023 kb on - strand at 766.092 kb on - strand at 766.092 kb on - strand at 766.092 kb on - strand at 766.097 kb on - strand at 766.144 kb on + strand at 766.165 kb on + strand at 766.166 kb on - strand at 766.237 kb on + strand, within BBR_RS20760 at 766.249 kb on + strand, within BBR_RS20760 at 766.259 kb on + strand, within BBR_RS20760 at 766.259 kb on + strand, within BBR_RS20760 at 766.283 kb on - strand at 766.330 kb on + strand at 766.436 kb on + strand, within BBR_RS20765 at 766.436 kb on - strand, within BBR_RS20765 at 766.436 kb on - strand, within BBR_RS20765 at 766.442 kb on - strand, within BBR_RS20765 at 766.470 kb on - strand, within BBR_RS20765 at 766.487 kb on + strand, within BBR_RS20765 at 766.503 kb on - strand, within BBR_RS20765 at 766.505 kb on + strand, within BBR_RS20765 at 766.505 kb on + strand, within BBR_RS20765 at 766.513 kb on - strand, within BBR_RS20765 at 766.513 kb on - strand, within BBR_RS20765 at 766.566 kb on + strand, within BBR_RS20765 at 766.595 kb on - strand, within BBR_RS20765 at 766.606 kb on + strand, within BBR_RS20765 at 766.626 kb on - strand at 766.629 kb on + strand at 766.644 kb on + strand at 766.645 kb on + strand at 766.661 kb on + strand at 766.661 kb on + strand at 766.685 kb on - strand at 766.701 kb on + strand at 766.714 kb on + strand at 766.725 kb on + strand at 766.758 kb on - strand at 766.795 kb on + strand, within BBR_RS20770 at 766.795 kb on + strand, within BBR_RS20770 at 766.796 kb on + strand, within BBR_RS20770 at 766.818 kb on + strand, within BBR_RS20770 at 766.826 kb on - strand, within BBR_RS20770 at 766.855 kb on - strand, within BBR_RS20770 at 766.917 kb on + strand at 766.925 kb on - strand at 766.936 kb on + strand at 766.984 kb on + strand at 766.984 kb on + strand at 767.027 kb on - strand at 767.083 kb on + strand at 767.175 kb on + strand at 767.183 kb on - strand at 767.183 kb on - strand at 767.247 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM remove 765,204 - BBR_RS13345 0.24 +2.4 765,232 + BBR_RS13345 0.27 +2.2 765,262 - BBR_RS13345 0.29 +0.7 765,269 - BBR_RS13345 0.30 +2.5 765,306 + BBR_RS13345 0.33 +0.7 765,349 - BBR_RS13345 0.37 +2.2 765,418 + BBR_RS13345 0.43 +0.2 765,425 + BBR_RS13345 0.43 +3.4 765,425 + BBR_RS13345 0.43 -0.9 765,433 - BBR_RS13345 0.44 -1.9 765,463 - BBR_RS13345 0.47 +0.4 765,482 + BBR_RS13345 0.48 -1.6 765,482 + BBR_RS13345 0.48 +1.7 765,488 - BBR_RS13345 0.49 +1.1 765,490 - BBR_RS13345 0.49 -0.9 765,490 - BBR_RS13345 0.49 -0.4 765,490 - BBR_RS13345 0.49 -0.2 765,492 - BBR_RS13345 0.49 -0.7 765,495 - BBR_RS13345 0.49 -1.4 765,543 + BBR_RS13345 0.54 +1.0 765,551 - BBR_RS13345 0.54 +1.2 765,551 - BBR_RS13345 0.54 +2.1 765,551 - BBR_RS13345 0.54 -0.7 765,551 - BBR_RS13345 0.54 +3.4 765,551 - BBR_RS13345 0.54 -0.1 765,551 - BBR_RS13345 0.54 -1.8 765,624 + BBR_RS13345 0.61 +1.8 765,632 - BBR_RS13345 0.61 -0.0 765,665 - BBR_RS13345 0.64 +2.7 765,671 - BBR_RS13345 0.65 +1.5 765,687 + BBR_RS13345 0.66 -1.6 765,699 - BBR_RS13345 0.67 +3.0 765,721 + BBR_RS13345 0.69 -0.3 765,727 - BBR_RS13345 0.70 -1.3 765,740 + BBR_RS13345 0.71 -1.3 765,810 - BBR_RS13345 0.77 +1.9 765,866 + BBR_RS13345 0.82 -0.8 765,894 - BBR_RS13345 0.84 -0.0 765,988 - +1.4 766,010 - +0.8 766,010 - +3.4 766,020 + -0.8 766,023 - +0.0 766,023 - -0.6 766,092 - +0.6 766,092 - -1.6 766,092 - -1.5 766,097 - -1.4 766,144 + -0.8 766,165 + -1.4 766,166 - +0.8 766,237 + BBR_RS20760 0.52 -1.4 766,249 + BBR_RS20760 0.68 -0.5 766,259 + BBR_RS20760 0.81 +1.2 766,259 + BBR_RS20760 0.81 +0.4 766,283 - -1.7 766,330 + +0.8 766,436 + BBR_RS20765 0.11 +1.2 766,436 - BBR_RS20765 0.11 -2.0 766,436 - BBR_RS20765 0.11 -2.1 766,442 - BBR_RS20765 0.14 +3.0 766,470 - BBR_RS20765 0.26 +0.5 766,487 + BBR_RS20765 0.34 -2.0 766,503 - BBR_RS20765 0.41 +1.1 766,505 + BBR_RS20765 0.42 -1.4 766,505 + BBR_RS20765 0.42 -0.3 766,513 - BBR_RS20765 0.45 -1.5 766,513 - BBR_RS20765 0.45 +0.4 766,566 + BBR_RS20765 0.69 -0.6 766,595 - BBR_RS20765 0.82 -1.4 766,606 + BBR_RS20765 0.87 +3.8 766,626 - -0.3 766,629 + +0.4 766,644 + +0.5 766,645 + +2.8 766,661 + +4.2 766,661 + -0.5 766,685 - -0.7 766,701 + +0.0 766,714 + -2.7 766,725 + +2.0 766,758 - +0.5 766,795 + BBR_RS20770 0.19 -0.4 766,795 + BBR_RS20770 0.19 -1.7 766,796 + BBR_RS20770 0.20 +0.5 766,818 + BBR_RS20770 0.32 +1.1 766,826 - BBR_RS20770 0.37 +0.0 766,855 - BBR_RS20770 0.54 -1.2 766,917 + -1.3 766,925 - -1.1 766,936 + -1.5 766,984 + -0.2 766,984 + -0.7 767,027 - -1.5 767,083 + +0.3 767,175 + +2.6 767,183 - -2.3 767,183 - -2.4 767,247 + +1.4
Or see this region's nucleotide sequence