Experiment: reconstituted MRS with Difucosyllactose 7.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12490 and BBR_RS20705 are separated by 366 nucleotides BBR_RS20705 and BBR_RS12495 are separated by 36 nucleotides
BBR_RS12490: BBR_RS12490 - hypothetical protein, at 554,298 to 554,694
_RS12490
BBR_RS20705: BBR_RS20705 - hypothetical protein, at 555,061 to 555,342
_RS20705
BBR_RS12495: BBR_RS12495 - sugar ABC transporter substrate-binding protein, at 555,379 to 556,728
_RS12495
Position (kb) 555
556 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 554.067 kb on - strand at 554.067 kb on - strand at 554.067 kb on - strand at 554.095 kb on + strand at 554.103 kb on + strand at 554.103 kb on - strand at 554.108 kb on + strand at 554.116 kb on - strand at 554.116 kb on - strand at 554.143 kb on + strand at 554.159 kb on - strand at 554.184 kb on - strand at 554.189 kb on + strand at 554.294 kb on + strand at 554.334 kb on + strand at 554.334 kb on + strand at 554.344 kb on + strand, within BBR_RS12490 at 554.344 kb on + strand, within BBR_RS12490 at 554.352 kb on - strand, within BBR_RS12490 at 554.352 kb on - strand, within BBR_RS12490 at 554.394 kb on - strand, within BBR_RS12490 at 554.396 kb on + strand, within BBR_RS12490 at 554.404 kb on - strand, within BBR_RS12490 at 554.411 kb on + strand, within BBR_RS12490 at 554.421 kb on - strand, within BBR_RS12490 at 554.435 kb on - strand, within BBR_RS12490 at 554.440 kb on - strand, within BBR_RS12490 at 554.443 kb on - strand, within BBR_RS12490 at 554.443 kb on - strand, within BBR_RS12490 at 554.501 kb on - strand, within BBR_RS12490 at 554.591 kb on - strand, within BBR_RS12490 at 554.643 kb on - strand, within BBR_RS12490 at 554.703 kb on + strand at 554.706 kb on + strand at 554.714 kb on - strand at 554.757 kb on - strand at 554.776 kb on + strand at 554.785 kb on - strand at 554.825 kb on + strand at 554.849 kb on - strand at 554.851 kb on + strand at 554.859 kb on - strand at 554.859 kb on - strand at 554.879 kb on - strand at 554.882 kb on + strand at 554.882 kb on + strand at 554.927 kb on - strand at 554.931 kb on + strand at 554.932 kb on + strand at 554.941 kb on + strand at 554.949 kb on - strand at 554.953 kb on + strand at 554.978 kb on - strand at 554.982 kb on + strand at 554.990 kb on - strand at 554.990 kb on - strand at 555.002 kb on - strand at 555.012 kb on - strand at 555.012 kb on - strand at 555.012 kb on - strand at 555.014 kb on + strand at 555.022 kb on - strand at 555.034 kb on - strand at 555.049 kb on - strand at 555.049 kb on - strand at 555.064 kb on + strand at 555.189 kb on + strand, within BBR_RS20705 at 555.189 kb on + strand, within BBR_RS20705 at 555.277 kb on - strand, within BBR_RS20705 at 555.277 kb on - strand, within BBR_RS20705 at 555.327 kb on + strand at 555.327 kb on + strand at 555.389 kb on + strand at 555.467 kb on + strand at 555.467 kb on - strand at 555.471 kb on + strand at 555.471 kb on + strand at 555.475 kb on - strand at 555.477 kb on + strand at 555.492 kb on + strand at 555.499 kb on + strand at 555.550 kb on + strand, within BBR_RS12495 at 555.570 kb on + strand, within BBR_RS12495 at 555.602 kb on - strand, within BBR_RS12495 at 555.604 kb on + strand, within BBR_RS12495 at 555.612 kb on - strand, within BBR_RS12495 at 555.625 kb on - strand, within BBR_RS12495 at 555.625 kb on - strand, within BBR_RS12495 at 555.627 kb on + strand, within BBR_RS12495 at 555.627 kb on + strand, within BBR_RS12495 at 555.627 kb on + strand, within BBR_RS12495 at 555.635 kb on - strand, within BBR_RS12495 at 555.686 kb on + strand, within BBR_RS12495 at 555.699 kb on - strand, within BBR_RS12495 at 555.721 kb on + strand, within BBR_RS12495 at 555.780 kb on - strand, within BBR_RS12495 at 555.782 kb on + strand, within BBR_RS12495 at 555.786 kb on + strand, within BBR_RS12495 at 555.809 kb on - strand, within BBR_RS12495 at 555.832 kb on + strand, within BBR_RS12495 at 555.840 kb on - strand, within BBR_RS12495 at 555.864 kb on - strand, within BBR_RS12495 at 555.888 kb on + strand, within BBR_RS12495 at 555.950 kb on + strand, within BBR_RS12495 at 555.969 kb on + strand, within BBR_RS12495 at 555.977 kb on + strand, within BBR_RS12495 at 555.984 kb on + strand, within BBR_RS12495 at 555.994 kb on + strand, within BBR_RS12495 at 555.999 kb on + strand, within BBR_RS12495 at 556.002 kb on - strand, within BBR_RS12495 at 556.002 kb on - strand, within BBR_RS12495 at 556.003 kb on + strand, within BBR_RS12495 at 556.003 kb on + strand, within BBR_RS12495 at 556.021 kb on - strand, within BBR_RS12495 at 556.021 kb on - strand, within BBR_RS12495 at 556.021 kb on - strand, within BBR_RS12495 at 556.028 kb on - strand, within BBR_RS12495 at 556.044 kb on + strand, within BBR_RS12495 at 556.065 kb on + strand, within BBR_RS12495 at 556.065 kb on + strand, within BBR_RS12495 at 556.065 kb on + strand, within BBR_RS12495 at 556.067 kb on - strand, within BBR_RS12495 at 556.067 kb on - strand, within BBR_RS12495 at 556.067 kb on - strand, within BBR_RS12495 at 556.105 kb on + strand, within BBR_RS12495 at 556.105 kb on + strand, within BBR_RS12495 at 556.118 kb on + strand, within BBR_RS12495 at 556.128 kb on + strand, within BBR_RS12495 at 556.128 kb on + strand, within BBR_RS12495 at 556.128 kb on - strand, within BBR_RS12495 at 556.135 kb on + strand, within BBR_RS12495 at 556.136 kb on - strand, within BBR_RS12495 at 556.145 kb on + strand, within BBR_RS12495 at 556.145 kb on + strand, within BBR_RS12495 at 556.145 kb on + strand, within BBR_RS12495 at 556.153 kb on + strand, within BBR_RS12495 at 556.153 kb on + strand, within BBR_RS12495 at 556.153 kb on - strand, within BBR_RS12495 at 556.153 kb on - strand, within BBR_RS12495 at 556.160 kb on + strand, within BBR_RS12495 at 556.189 kb on + strand, within BBR_RS12495 at 556.243 kb on - strand, within BBR_RS12495 at 556.243 kb on - strand, within BBR_RS12495 at 556.341 kb on + strand, within BBR_RS12495
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM remove 554,067 - +1.8 554,067 - -1.8 554,067 - -0.8 554,095 + -1.1 554,103 + +1.3 554,103 - -2.6 554,108 + +2.9 554,116 - -1.1 554,116 - +0.6 554,143 + -1.3 554,159 - -1.1 554,184 - +4.4 554,189 + +3.5 554,294 + +1.8 554,334 + -1.0 554,334 + -0.3 554,344 + BBR_RS12490 0.12 +1.3 554,344 + BBR_RS12490 0.12 -1.2 554,352 - BBR_RS12490 0.14 +2.8 554,352 - BBR_RS12490 0.14 +4.1 554,394 - BBR_RS12490 0.24 -2.2 554,396 + BBR_RS12490 0.25 +0.2 554,404 - BBR_RS12490 0.27 +2.9 554,411 + BBR_RS12490 0.28 -0.2 554,421 - BBR_RS12490 0.31 +0.2 554,435 - BBR_RS12490 0.35 +2.2 554,440 - BBR_RS12490 0.36 +0.1 554,443 - BBR_RS12490 0.37 -1.5 554,443 - BBR_RS12490 0.37 -0.9 554,501 - BBR_RS12490 0.51 +1.9 554,591 - BBR_RS12490 0.74 -1.3 554,643 - BBR_RS12490 0.87 -1.7 554,703 + -0.6 554,706 + +2.0 554,714 - -0.7 554,757 - -0.9 554,776 + +2.1 554,785 - -1.9 554,825 + +0.2 554,849 - -1.5 554,851 + -1.0 554,859 - -1.1 554,859 - +0.1 554,879 - -0.4 554,882 + +3.1 554,882 + -0.1 554,927 - -2.0 554,931 + -0.2 554,932 + -0.5 554,941 + -1.0 554,949 - +0.6 554,953 + -0.6 554,978 - +3.2 554,982 + -0.6 554,990 - -1.1 554,990 - -1.0 555,002 - +3.3 555,012 - +0.9 555,012 - +4.2 555,012 - -1.3 555,014 + +2.3 555,022 - +3.1 555,034 - -1.6 555,049 - +2.6 555,049 - -0.6 555,064 + -0.5 555,189 + BBR_RS20705 0.45 -0.1 555,189 + BBR_RS20705 0.45 +0.0 555,277 - BBR_RS20705 0.77 -1.2 555,277 - BBR_RS20705 0.77 +1.1 555,327 + +1.9 555,327 + +2.4 555,389 + -1.2 555,467 + -1.3 555,467 - +1.1 555,471 + +0.4 555,471 + -0.8 555,475 - -0.9 555,477 + +0.4 555,492 + +0.1 555,499 + -0.8 555,550 + BBR_RS12495 0.13 +0.7 555,570 + BBR_RS12495 0.14 +0.5 555,602 - BBR_RS12495 0.17 -1.2 555,604 + BBR_RS12495 0.17 +2.2 555,612 - BBR_RS12495 0.17 -0.3 555,625 - BBR_RS12495 0.18 -0.1 555,625 - BBR_RS12495 0.18 -0.3 555,627 + BBR_RS12495 0.18 -0.3 555,627 + BBR_RS12495 0.18 -0.4 555,627 + BBR_RS12495 0.18 +1.3 555,635 - BBR_RS12495 0.19 +1.2 555,686 + BBR_RS12495 0.23 +1.6 555,699 - BBR_RS12495 0.24 +2.0 555,721 + BBR_RS12495 0.25 +1.9 555,780 - BBR_RS12495 0.30 -0.7 555,782 + BBR_RS12495 0.30 +1.7 555,786 + BBR_RS12495 0.30 +0.4 555,809 - BBR_RS12495 0.32 -1.1 555,832 + BBR_RS12495 0.34 -0.3 555,840 - BBR_RS12495 0.34 -1.3 555,864 - BBR_RS12495 0.36 +0.8 555,888 + BBR_RS12495 0.38 +3.3 555,950 + BBR_RS12495 0.42 -1.2 555,969 + BBR_RS12495 0.44 +1.7 555,977 + BBR_RS12495 0.44 +0.6 555,984 + BBR_RS12495 0.45 -1.0 555,994 + BBR_RS12495 0.46 -0.5 555,999 + BBR_RS12495 0.46 +0.8 556,002 - BBR_RS12495 0.46 +3.4 556,002 - BBR_RS12495 0.46 -0.7 556,003 + BBR_RS12495 0.46 +2.8 556,003 + BBR_RS12495 0.46 +1.1 556,021 - BBR_RS12495 0.48 -1.1 556,021 - BBR_RS12495 0.48 +4.9 556,021 - BBR_RS12495 0.48 -0.5 556,028 - BBR_RS12495 0.48 +1.8 556,044 + BBR_RS12495 0.49 +0.7 556,065 + BBR_RS12495 0.51 +0.4 556,065 + BBR_RS12495 0.51 -0.6 556,065 + BBR_RS12495 0.51 +3.4 556,067 - BBR_RS12495 0.51 -0.8 556,067 - BBR_RS12495 0.51 -0.0 556,067 - BBR_RS12495 0.51 -0.9 556,105 + BBR_RS12495 0.54 -1.1 556,105 + BBR_RS12495 0.54 -1.3 556,118 + BBR_RS12495 0.55 -1.8 556,128 + BBR_RS12495 0.55 +3.6 556,128 + BBR_RS12495 0.55 -2.1 556,128 - BBR_RS12495 0.55 +0.6 556,135 + BBR_RS12495 0.56 +0.6 556,136 - BBR_RS12495 0.56 +0.2 556,145 + BBR_RS12495 0.57 +0.4 556,145 + BBR_RS12495 0.57 -0.9 556,145 + BBR_RS12495 0.57 -2.0 556,153 + BBR_RS12495 0.57 +0.2 556,153 + BBR_RS12495 0.57 +1.4 556,153 - BBR_RS12495 0.57 -0.9 556,153 - BBR_RS12495 0.57 -0.2 556,160 + BBR_RS12495 0.58 +2.2 556,189 + BBR_RS12495 0.60 -1.4 556,243 - BBR_RS12495 0.64 -0.0 556,243 - BBR_RS12495 0.64 -1.0 556,341 + BBR_RS12495 0.71 -0.7
Or see this region's nucleotide sequence