Experiment: reconstituted MRS with Difucosyllactose 7.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12310 and BBR_RS20690 are separated by 38 nucleotides BBR_RS20690 and BBR_RS12315 are separated by 115 nucleotides
BBR_RS12310: BBR_RS12310 - signal recognition particle protein, at 512,241 to 513,896
_RS12310
BBR_RS20690: BBR_RS20690 - hypothetical protein, at 513,935 to 514,135
_RS20690
BBR_RS12315: BBR_RS12315 - cation transporter, at 514,251 to 515,159
_RS12315
Position (kb) 513
514
515 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 513.898 kb on - strand at 513.979 kb on - strand, within BBR_RS20690 at 513.979 kb on - strand, within BBR_RS20690 at 514.043 kb on + strand, within BBR_RS20690 at 514.090 kb on - strand, within BBR_RS20690 at 514.152 kb on + strand at 514.166 kb on + strand at 514.166 kb on + strand at 514.200 kb on + strand at 514.281 kb on + strand at 514.289 kb on - strand at 514.319 kb on - strand at 514.359 kb on + strand, within BBR_RS12315 at 514.437 kb on + strand, within BBR_RS12315 at 514.439 kb on + strand, within BBR_RS12315 at 514.439 kb on + strand, within BBR_RS12315 at 514.441 kb on + strand, within BBR_RS12315 at 514.445 kb on - strand, within BBR_RS12315 at 514.445 kb on - strand, within BBR_RS12315 at 514.445 kb on - strand, within BBR_RS12315 at 514.445 kb on - strand, within BBR_RS12315 at 514.445 kb on - strand, within BBR_RS12315 at 514.452 kb on - strand, within BBR_RS12315 at 514.469 kb on + strand, within BBR_RS12315 at 514.482 kb on - strand, within BBR_RS12315 at 514.482 kb on - strand, within BBR_RS12315 at 514.496 kb on + strand, within BBR_RS12315 at 514.511 kb on - strand, within BBR_RS12315 at 514.534 kb on - strand, within BBR_RS12315 at 514.550 kb on - strand, within BBR_RS12315 at 514.557 kb on + strand, within BBR_RS12315 at 514.572 kb on + strand, within BBR_RS12315 at 514.592 kb on + strand, within BBR_RS12315 at 514.592 kb on + strand, within BBR_RS12315 at 514.600 kb on - strand, within BBR_RS12315 at 514.631 kb on - strand, within BBR_RS12315 at 514.642 kb on + strand, within BBR_RS12315 at 514.648 kb on + strand, within BBR_RS12315 at 514.650 kb on - strand, within BBR_RS12315 at 514.650 kb on - strand, within BBR_RS12315 at 514.650 kb on - strand, within BBR_RS12315 at 514.656 kb on - strand, within BBR_RS12315 at 514.691 kb on + strand, within BBR_RS12315 at 514.691 kb on + strand, within BBR_RS12315 at 514.691 kb on + strand, within BBR_RS12315 at 514.691 kb on + strand, within BBR_RS12315 at 514.691 kb on + strand, within BBR_RS12315 at 514.691 kb on + strand, within BBR_RS12315 at 514.699 kb on - strand, within BBR_RS12315 at 514.711 kb on - strand, within BBR_RS12315 at 514.758 kb on + strand, within BBR_RS12315 at 514.761 kb on - strand, within BBR_RS12315 at 514.768 kb on + strand, within BBR_RS12315 at 514.800 kb on + strand, within BBR_RS12315 at 514.822 kb on - strand, within BBR_RS12315 at 514.822 kb on - strand, within BBR_RS12315 at 514.827 kb on - strand, within BBR_RS12315 at 514.827 kb on - strand, within BBR_RS12315 at 514.847 kb on - strand, within BBR_RS12315 at 514.852 kb on - strand, within BBR_RS12315 at 514.864 kb on + strand, within BBR_RS12315 at 514.864 kb on + strand, within BBR_RS12315 at 514.872 kb on - strand, within BBR_RS12315 at 514.946 kb on + strand, within BBR_RS12315 at 514.975 kb on + strand, within BBR_RS12315 at 515.021 kb on + strand, within BBR_RS12315 at 515.021 kb on + strand, within BBR_RS12315 at 515.024 kb on - strand, within BBR_RS12315 at 515.026 kb on + strand, within BBR_RS12315 at 515.032 kb on - strand, within BBR_RS12315 at 515.039 kb on - strand, within BBR_RS12315 at 515.058 kb on - strand, within BBR_RS12315 at 515.058 kb on - strand, within BBR_RS12315 at 515.109 kb on + strand at 515.121 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM remove 513,898 - -1.0 513,979 - BBR_RS20690 0.22 -0.1 513,979 - BBR_RS20690 0.22 -0.1 514,043 + BBR_RS20690 0.54 -1.4 514,090 - BBR_RS20690 0.77 -0.2 514,152 + +0.6 514,166 + +3.8 514,166 + -0.4 514,200 + +3.7 514,281 + -0.1 514,289 - -0.2 514,319 - -1.1 514,359 + BBR_RS12315 0.12 +2.7 514,437 + BBR_RS12315 0.20 -2.6 514,439 + BBR_RS12315 0.21 +3.5 514,439 + BBR_RS12315 0.21 -0.4 514,441 + BBR_RS12315 0.21 -0.2 514,445 - BBR_RS12315 0.21 -0.7 514,445 - BBR_RS12315 0.21 +3.2 514,445 - BBR_RS12315 0.21 -1.3 514,445 - BBR_RS12315 0.21 -0.6 514,445 - BBR_RS12315 0.21 -1.1 514,452 - BBR_RS12315 0.22 -0.8 514,469 + BBR_RS12315 0.24 -0.8 514,482 - BBR_RS12315 0.25 -0.7 514,482 - BBR_RS12315 0.25 -1.0 514,496 + BBR_RS12315 0.27 -0.5 514,511 - BBR_RS12315 0.29 +1.7 514,534 - BBR_RS12315 0.31 +1.7 514,550 - BBR_RS12315 0.33 -0.2 514,557 + BBR_RS12315 0.34 +1.8 514,572 + BBR_RS12315 0.35 +0.9 514,592 + BBR_RS12315 0.38 -2.0 514,592 + BBR_RS12315 0.38 +2.4 514,600 - BBR_RS12315 0.38 -0.2 514,631 - BBR_RS12315 0.42 -1.1 514,642 + BBR_RS12315 0.43 +2.4 514,648 + BBR_RS12315 0.44 +1.3 514,650 - BBR_RS12315 0.44 -0.5 514,650 - BBR_RS12315 0.44 +2.1 514,650 - BBR_RS12315 0.44 -1.1 514,656 - BBR_RS12315 0.45 -1.0 514,691 + BBR_RS12315 0.48 -1.1 514,691 + BBR_RS12315 0.48 +0.3 514,691 + BBR_RS12315 0.48 +2.7 514,691 + BBR_RS12315 0.48 -1.1 514,691 + BBR_RS12315 0.48 +0.4 514,691 + BBR_RS12315 0.48 +0.5 514,699 - BBR_RS12315 0.49 -0.1 514,711 - BBR_RS12315 0.51 -0.3 514,758 + BBR_RS12315 0.56 -0.9 514,761 - BBR_RS12315 0.56 -0.2 514,768 + BBR_RS12315 0.57 +0.2 514,800 + BBR_RS12315 0.60 +2.9 514,822 - BBR_RS12315 0.63 +1.6 514,822 - BBR_RS12315 0.63 -1.3 514,827 - BBR_RS12315 0.63 +0.7 514,827 - BBR_RS12315 0.63 -0.1 514,847 - BBR_RS12315 0.66 +0.3 514,852 - BBR_RS12315 0.66 +0.8 514,864 + BBR_RS12315 0.67 -1.2 514,864 + BBR_RS12315 0.67 -1.1 514,872 - BBR_RS12315 0.68 +0.1 514,946 + BBR_RS12315 0.76 -0.1 514,975 + BBR_RS12315 0.80 -0.9 515,021 + BBR_RS12315 0.85 -0.7 515,021 + BBR_RS12315 0.85 -0.4 515,024 - BBR_RS12315 0.85 +1.7 515,026 + BBR_RS12315 0.85 +2.9 515,032 - BBR_RS12315 0.86 -0.8 515,039 - BBR_RS12315 0.87 -0.9 515,058 - BBR_RS12315 0.89 -0.9 515,058 - BBR_RS12315 0.89 +2.6 515,109 + +1.6 515,121 + +1.9
Or see this region's nucleotide sequence