Experiment: reconstituted MRS with Difucosyllactose 7.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18000 and BBR_RS18005 are separated by 163 nucleotides BBR_RS18005 and BBR_RS18010 are separated by 164 nucleotides
BBR_RS18000: BBR_RS18000 - type I methionyl aminopeptidase, at 1,826,469 to 1,827,251
_RS18000
BBR_RS18005: BBR_RS18005 - hypothetical protein, at 1,827,415 to 1,828,299
_RS18005
BBR_RS18010: BBR_RS18010 - zinc metalloprotease, at 1,828,464 to 1,830,647
_RS18010
Position (kb)
1827
1828
1829 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1826.462 kb on + strand at 1826.482 kb on - strand at 1827.287 kb on - strand at 1827.328 kb on - strand at 1827.376 kb on - strand at 1827.520 kb on - strand, within BBR_RS18005 at 1827.520 kb on - strand, within BBR_RS18005 at 1827.520 kb on - strand, within BBR_RS18005 at 1827.521 kb on + strand, within BBR_RS18005 at 1827.610 kb on - strand, within BBR_RS18005 at 1827.644 kb on - strand, within BBR_RS18005 at 1827.669 kb on - strand, within BBR_RS18005 at 1827.674 kb on - strand, within BBR_RS18005 at 1827.681 kb on + strand, within BBR_RS18005 at 1827.734 kb on - strand, within BBR_RS18005 at 1827.801 kb on + strand, within BBR_RS18005 at 1827.807 kb on - strand, within BBR_RS18005 at 1827.857 kb on - strand, within BBR_RS18005 at 1827.945 kb on - strand, within BBR_RS18005 at 1827.974 kb on - strand, within BBR_RS18005 at 1828.000 kb on - strand, within BBR_RS18005 at 1828.034 kb on - strand, within BBR_RS18005 at 1828.079 kb on + strand, within BBR_RS18005 at 1828.079 kb on + strand, within BBR_RS18005 at 1828.125 kb on - strand, within BBR_RS18005 at 1828.125 kb on - strand, within BBR_RS18005 at 1828.127 kb on + strand, within BBR_RS18005 at 1828.210 kb on + strand, within BBR_RS18005 at 1828.224 kb on + strand at 1828.230 kb on - strand at 1828.232 kb on - strand at 1828.235 kb on - strand at 1828.255 kb on - strand at 1828.292 kb on - strand at 1828.305 kb on - strand at 1828.315 kb on - strand at 1828.320 kb on - strand at 1828.320 kb on - strand at 1828.341 kb on + strand at 1828.352 kb on - strand at 1828.439 kb on - strand at 1828.439 kb on - strand at 1828.469 kb on - strand at 1828.552 kb on - strand at 1828.584 kb on - strand at 1828.610 kb on - strand at 1828.612 kb on - strand at 1828.612 kb on - strand at 1828.667 kb on - strand at 1828.695 kb on + strand, within BBR_RS18010 at 1828.695 kb on + strand, within BBR_RS18010 at 1828.700 kb on + strand, within BBR_RS18010 at 1828.720 kb on - strand, within BBR_RS18010 at 1828.720 kb on - strand, within BBR_RS18010 at 1828.741 kb on + strand, within BBR_RS18010 at 1828.748 kb on - strand, within BBR_RS18010 at 1828.749 kb on - strand, within BBR_RS18010 at 1828.759 kb on - strand, within BBR_RS18010 at 1828.761 kb on + strand, within BBR_RS18010 at 1828.762 kb on + strand, within BBR_RS18010 at 1828.795 kb on - strand, within BBR_RS18010 at 1828.800 kb on - strand, within BBR_RS18010 at 1828.800 kb on - strand, within BBR_RS18010 at 1828.800 kb on - strand, within BBR_RS18010 at 1828.819 kb on + strand, within BBR_RS18010 at 1828.819 kb on - strand, within BBR_RS18010 at 1828.827 kb on - strand, within BBR_RS18010 at 1828.827 kb on - strand, within BBR_RS18010 at 1828.836 kb on + strand, within BBR_RS18010 at 1828.836 kb on - strand, within BBR_RS18010 at 1828.837 kb on + strand, within BBR_RS18010 at 1828.865 kb on + strand, within BBR_RS18010 at 1828.886 kb on + strand, within BBR_RS18010 at 1828.894 kb on - strand, within BBR_RS18010 at 1828.894 kb on - strand, within BBR_RS18010 at 1828.908 kb on - strand, within BBR_RS18010 at 1828.935 kb on + strand, within BBR_RS18010 at 1828.935 kb on + strand, within BBR_RS18010 at 1828.935 kb on + strand, within BBR_RS18010 at 1828.935 kb on + strand, within BBR_RS18010 at 1828.935 kb on + strand, within BBR_RS18010 at 1828.943 kb on - strand, within BBR_RS18010 at 1828.952 kb on + strand, within BBR_RS18010 at 1828.962 kb on + strand, within BBR_RS18010 at 1828.962 kb on + strand, within BBR_RS18010 at 1828.962 kb on + strand, within BBR_RS18010 at 1828.962 kb on + strand, within BBR_RS18010 at 1828.970 kb on - strand, within BBR_RS18010 at 1828.970 kb on - strand, within BBR_RS18010 at 1828.974 kb on + strand, within BBR_RS18010 at 1828.998 kb on + strand, within BBR_RS18010 at 1828.998 kb on + strand, within BBR_RS18010 at 1829.008 kb on - strand, within BBR_RS18010 at 1829.026 kb on + strand, within BBR_RS18010 at 1829.028 kb on + strand, within BBR_RS18010 at 1829.036 kb on - strand, within BBR_RS18010 at 1829.068 kb on - strand, within BBR_RS18010 at 1829.086 kb on + strand, within BBR_RS18010 at 1829.091 kb on + strand, within BBR_RS18010 at 1829.093 kb on + strand, within BBR_RS18010 at 1829.104 kb on - strand, within BBR_RS18010 at 1829.109 kb on - strand, within BBR_RS18010 at 1829.109 kb on - strand, within BBR_RS18010 at 1829.141 kb on + strand, within BBR_RS18010 at 1829.205 kb on + strand, within BBR_RS18010 at 1829.208 kb on - strand, within BBR_RS18010 at 1829.212 kb on + strand, within BBR_RS18010 at 1829.221 kb on - strand, within BBR_RS18010 at 1829.242 kb on + strand, within BBR_RS18010 at 1829.293 kb on + strand, within BBR_RS18010
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM remove 1,826,462 + +0.8 1,826,482 - -0.3 1,827,287 - +0.8 1,827,328 - -0.6 1,827,376 - -1.1 1,827,520 - BBR_RS18005 0.12 +3.6 1,827,520 - BBR_RS18005 0.12 +3.5 1,827,520 - BBR_RS18005 0.12 +4.1 1,827,521 + BBR_RS18005 0.12 +4.2 1,827,610 - BBR_RS18005 0.22 -1.0 1,827,644 - BBR_RS18005 0.26 +3.0 1,827,669 - BBR_RS18005 0.29 -0.8 1,827,674 - BBR_RS18005 0.29 +2.4 1,827,681 + BBR_RS18005 0.30 +2.3 1,827,734 - BBR_RS18005 0.36 +4.1 1,827,801 + BBR_RS18005 0.44 +0.9 1,827,807 - BBR_RS18005 0.44 +3.2 1,827,857 - BBR_RS18005 0.50 +2.0 1,827,945 - BBR_RS18005 0.60 +1.9 1,827,974 - BBR_RS18005 0.63 -2.1 1,828,000 - BBR_RS18005 0.66 +2.1 1,828,034 - BBR_RS18005 0.70 +4.4 1,828,079 + BBR_RS18005 0.75 -0.4 1,828,079 + BBR_RS18005 0.75 +3.6 1,828,125 - BBR_RS18005 0.80 -0.5 1,828,125 - BBR_RS18005 0.80 +0.8 1,828,127 + BBR_RS18005 0.80 +0.9 1,828,210 + BBR_RS18005 0.90 +1.5 1,828,224 + +0.6 1,828,230 - +3.1 1,828,232 - -0.8 1,828,235 - +2.8 1,828,255 - -1.4 1,828,292 - +4.2 1,828,305 - +0.4 1,828,315 - -0.7 1,828,320 - -0.7 1,828,320 - +3.0 1,828,341 + +2.7 1,828,352 - -1.1 1,828,439 - -1.3 1,828,439 - -0.5 1,828,469 - +3.1 1,828,552 - -1.6 1,828,584 - -2.1 1,828,610 - +2.4 1,828,612 - +0.3 1,828,612 - +1.9 1,828,667 - -0.1 1,828,695 + BBR_RS18010 0.11 -0.7 1,828,695 + BBR_RS18010 0.11 +2.5 1,828,700 + BBR_RS18010 0.11 +1.9 1,828,720 - BBR_RS18010 0.12 -0.4 1,828,720 - BBR_RS18010 0.12 -1.5 1,828,741 + BBR_RS18010 0.13 -0.0 1,828,748 - BBR_RS18010 0.13 -1.5 1,828,749 - BBR_RS18010 0.13 +2.6 1,828,759 - BBR_RS18010 0.14 +2.1 1,828,761 + BBR_RS18010 0.14 +2.4 1,828,762 + BBR_RS18010 0.14 -1.6 1,828,795 - BBR_RS18010 0.15 +2.1 1,828,800 - BBR_RS18010 0.15 -1.6 1,828,800 - BBR_RS18010 0.15 -2.0 1,828,800 - BBR_RS18010 0.15 -1.9 1,828,819 + BBR_RS18010 0.16 +1.9 1,828,819 - BBR_RS18010 0.16 +2.8 1,828,827 - BBR_RS18010 0.17 +1.7 1,828,827 - BBR_RS18010 0.17 +2.0 1,828,836 + BBR_RS18010 0.17 +0.3 1,828,836 - BBR_RS18010 0.17 -2.8 1,828,837 + BBR_RS18010 0.17 -0.1 1,828,865 + BBR_RS18010 0.18 +2.3 1,828,886 + BBR_RS18010 0.19 +0.4 1,828,894 - BBR_RS18010 0.20 -0.2 1,828,894 - BBR_RS18010 0.20 -1.8 1,828,908 - BBR_RS18010 0.20 +2.0 1,828,935 + BBR_RS18010 0.22 -2.3 1,828,935 + BBR_RS18010 0.22 +2.0 1,828,935 + BBR_RS18010 0.22 -1.2 1,828,935 + BBR_RS18010 0.22 +1.4 1,828,935 + BBR_RS18010 0.22 -0.7 1,828,943 - BBR_RS18010 0.22 +2.8 1,828,952 + BBR_RS18010 0.22 +2.0 1,828,962 + BBR_RS18010 0.23 -0.4 1,828,962 + BBR_RS18010 0.23 +2.5 1,828,962 + BBR_RS18010 0.23 -1.2 1,828,962 + BBR_RS18010 0.23 -0.4 1,828,970 - BBR_RS18010 0.23 -0.7 1,828,970 - BBR_RS18010 0.23 +0.4 1,828,974 + BBR_RS18010 0.23 +1.1 1,828,998 + BBR_RS18010 0.24 +0.1 1,828,998 + BBR_RS18010 0.24 -1.8 1,829,008 - BBR_RS18010 0.25 +1.5 1,829,026 + BBR_RS18010 0.26 -1.6 1,829,028 + BBR_RS18010 0.26 +2.2 1,829,036 - BBR_RS18010 0.26 +0.3 1,829,068 - BBR_RS18010 0.28 +3.1 1,829,086 + BBR_RS18010 0.28 -1.0 1,829,091 + BBR_RS18010 0.29 -1.8 1,829,093 + BBR_RS18010 0.29 +3.4 1,829,104 - BBR_RS18010 0.29 +3.6 1,829,109 - BBR_RS18010 0.30 +2.2 1,829,109 - BBR_RS18010 0.30 +0.3 1,829,141 + BBR_RS18010 0.31 +1.2 1,829,205 + BBR_RS18010 0.34 +2.5 1,829,208 - BBR_RS18010 0.34 +2.7 1,829,212 + BBR_RS18010 0.34 -0.3 1,829,221 - BBR_RS18010 0.35 -1.2 1,829,242 + BBR_RS18010 0.36 -1.4 1,829,293 + BBR_RS18010 0.38 -0.6
Or see this region's nucleotide sequence