Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS17940

Experiment: reconstituted MRS with Difucosyllactose 7.9 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS17935 and BBR_RS17940 are separated by 136 nucleotidesBBR_RS17940 and BBR_RS17945 are separated by 187 nucleotidesBBR_RS17945 and BBR_RS17950 are separated by 91 nucleotidesBBR_RS17950 and BBR_RS17955 are separated by 190 nucleotides BBR_RS17935: BBR_RS17935 - long-chain fatty acid--CoA ligase, at 1,810,043 to 1,811,899 _RS17935 BBR_RS17940: BBR_RS17940 - large conductance mechanosensitive channel protein MscL, at 1,812,036 to 1,812,542 _RS17940 BBR_RS17945: BBR_RS17945 - nucleoside deoxyribosyltransferase, at 1,812,730 to 1,813,158 _RS17945 BBR_RS17950: BBR_RS17950 - tRNA-Arg, at 1,813,250 to 1,813,322 _RS17950 BBR_RS17955: BBR_RS17955 - endonuclease/exonuclease/phosphatase family protein, at 1,813,513 to 1,814,652 _RS17955 Position (kb) 1812 1813Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1811.042 kb on - strand, within BBR_RS17935at 1811.066 kb on + strand, within BBR_RS17935at 1811.079 kb on + strand, within BBR_RS17935at 1811.116 kb on + strand, within BBR_RS17935at 1811.116 kb on + strand, within BBR_RS17935at 1811.116 kb on + strand, within BBR_RS17935at 1811.154 kb on + strand, within BBR_RS17935at 1811.163 kb on + strand, within BBR_RS17935at 1811.163 kb on + strand, within BBR_RS17935at 1811.164 kb on - strand, within BBR_RS17935at 1811.171 kb on - strand, within BBR_RS17935at 1811.171 kb on - strand, within BBR_RS17935at 1811.179 kb on - strand, within BBR_RS17935at 1811.208 kb on + strand, within BBR_RS17935at 1811.208 kb on + strand, within BBR_RS17935at 1811.213 kb on + strand, within BBR_RS17935at 1811.216 kb on + strand, within BBR_RS17935at 1811.221 kb on - strand, within BBR_RS17935at 1811.224 kb on - strand, within BBR_RS17935at 1811.423 kb on + strand, within BBR_RS17935at 1811.428 kb on + strand, within BBR_RS17935at 1811.428 kb on + strand, within BBR_RS17935at 1811.431 kb on - strand, within BBR_RS17935at 1811.431 kb on - strand, within BBR_RS17935at 1811.431 kb on - strand, within BBR_RS17935at 1811.431 kb on - strand, within BBR_RS17935at 1811.451 kb on + strand, within BBR_RS17935at 1811.472 kb on + strand, within BBR_RS17935at 1811.500 kb on + strand, within BBR_RS17935at 1811.500 kb on + strand, within BBR_RS17935at 1811.533 kb on - strand, within BBR_RS17935at 1811.586 kb on + strand, within BBR_RS17935at 1811.591 kb on + strand, within BBR_RS17935at 1811.591 kb on + strand, within BBR_RS17935at 1811.591 kb on + strand, within BBR_RS17935at 1811.599 kb on - strand, within BBR_RS17935at 1811.606 kb on + strand, within BBR_RS17935at 1811.609 kb on - strand, within BBR_RS17935at 1811.637 kb on + strand, within BBR_RS17935at 1811.668 kb on + strand, within BBR_RS17935at 1811.670 kb on + strand, within BBR_RS17935at 1811.671 kb on - strand, within BBR_RS17935at 1811.671 kb on - strand, within BBR_RS17935at 1811.678 kb on - strand, within BBR_RS17935at 1811.710 kb on - strand, within BBR_RS17935at 1811.756 kb on + strandat 1811.803 kb on + strandat 1811.811 kb on - strandat 1811.813 kb on - strandat 1811.835 kb on + strandat 1811.835 kb on - strandat 1811.848 kb on + strandat 1811.873 kb on - strandat 1811.921 kb on + strandat 1811.964 kb on - strandat 1811.964 kb on - strandat 1811.965 kb on + strandat 1811.973 kb on - strandat 1812.028 kb on + strandat 1812.028 kb on + strandat 1812.035 kb on - strandat 1812.035 kb on - strandat 1812.036 kb on - strandat 1812.036 kb on - strandat 1812.036 kb on - strandat 1812.096 kb on + strand, within BBR_RS17940at 1812.110 kb on + strand, within BBR_RS17940at 1812.137 kb on - strand, within BBR_RS17940at 1812.144 kb on - strand, within BBR_RS17940at 1812.148 kb on - strand, within BBR_RS17940at 1812.149 kb on - strand, within BBR_RS17940at 1812.177 kb on + strand, within BBR_RS17940at 1812.200 kb on - strand, within BBR_RS17940at 1812.239 kb on + strand, within BBR_RS17940at 1812.242 kb on - strand, within BBR_RS17940at 1812.247 kb on - strand, within BBR_RS17940at 1812.247 kb on - strand, within BBR_RS17940at 1812.282 kb on - strand, within BBR_RS17940at 1812.378 kb on + strand, within BBR_RS17940at 1812.378 kb on + strand, within BBR_RS17940at 1812.378 kb on + strand, within BBR_RS17940at 1812.386 kb on - strand, within BBR_RS17940at 1812.386 kb on - strand, within BBR_RS17940at 1812.386 kb on - strand, within BBR_RS17940at 1812.386 kb on - strand, within BBR_RS17940at 1812.386 kb on - strand, within BBR_RS17940at 1812.449 kb on + strand, within BBR_RS17940at 1812.489 kb on + strand, within BBR_RS17940at 1812.536 kb on - strandat 1812.548 kb on + strandat 1812.548 kb on + strandat 1812.556 kb on - strandat 1812.698 kb on + strandat 1812.706 kb on - strandat 1812.808 kb on + strand, within BBR_RS17945at 1812.852 kb on + strand, within BBR_RS17945at 1812.852 kb on + strand, within BBR_RS17945at 1812.897 kb on + strand, within BBR_RS17945at 1812.920 kb on + strand, within BBR_RS17945at 1812.920 kb on + strand, within BBR_RS17945at 1812.920 kb on + strand, within BBR_RS17945at 1812.928 kb on + strand, within BBR_RS17945at 1812.928 kb on + strand, within BBR_RS17945at 1812.936 kb on - strand, within BBR_RS17945at 1812.936 kb on - strand, within BBR_RS17945at 1813.000 kb on - strand, within BBR_RS17945at 1813.004 kb on + strand, within BBR_RS17945at 1813.037 kb on + strand, within BBR_RS17945at 1813.049 kb on + strand, within BBR_RS17945at 1813.082 kb on - strand, within BBR_RS17945at 1813.114 kb on - strand, within BBR_RS17945at 1813.114 kb on - strand, within BBR_RS17945at 1813.135 kb on + strandat 1813.167 kb on - strandat 1813.173 kb on + strandat 1813.181 kb on - strandat 1813.221 kb on - strandat 1813.386 kb on - strandat 1813.391 kb on - strandat 1813.438 kb on - strandat 1813.450 kb on + strandat 1813.519 kb on - strandat 1813.519 kb on - strandat 1813.532 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM
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1,811,042 - BBR_RS17935 0.54 -1.1
1,811,066 + BBR_RS17935 0.55 -1.0
1,811,079 + BBR_RS17935 0.56 +0.7
1,811,116 + BBR_RS17935 0.58 -1.6
1,811,116 + BBR_RS17935 0.58 +2.6
1,811,116 + BBR_RS17935 0.58 +0.2
1,811,154 + BBR_RS17935 0.60 +1.4
1,811,163 + BBR_RS17935 0.60 -1.8
1,811,163 + BBR_RS17935 0.60 +2.0
1,811,164 - BBR_RS17935 0.60 +0.4
1,811,171 - BBR_RS17935 0.61 +2.6
1,811,171 - BBR_RS17935 0.61 +4.7
1,811,179 - BBR_RS17935 0.61 -2.6
1,811,208 + BBR_RS17935 0.63 +4.0
1,811,208 + BBR_RS17935 0.63 +1.4
1,811,213 + BBR_RS17935 0.63 +0.1
1,811,216 + BBR_RS17935 0.63 +1.4
1,811,221 - BBR_RS17935 0.63 +3.5
1,811,224 - BBR_RS17935 0.64 +0.2
1,811,423 + BBR_RS17935 0.74 -1.4
1,811,428 + BBR_RS17935 0.75 -1.0
1,811,428 + BBR_RS17935 0.75 -0.1
1,811,431 - BBR_RS17935 0.75 +3.3
1,811,431 - BBR_RS17935 0.75 +2.2
1,811,431 - BBR_RS17935 0.75 -0.5
1,811,431 - BBR_RS17935 0.75 -1.8
1,811,451 + BBR_RS17935 0.76 -0.5
1,811,472 + BBR_RS17935 0.77 -1.9
1,811,500 + BBR_RS17935 0.78 +2.1
1,811,500 + BBR_RS17935 0.78 -1.3
1,811,533 - BBR_RS17935 0.80 +2.2
1,811,586 + BBR_RS17935 0.83 +1.6
1,811,591 + BBR_RS17935 0.83 -0.8
1,811,591 + BBR_RS17935 0.83 -1.3
1,811,591 + BBR_RS17935 0.83 -0.7
1,811,599 - BBR_RS17935 0.84 -1.4
1,811,606 + BBR_RS17935 0.84 +0.2
1,811,609 - BBR_RS17935 0.84 -0.4
1,811,637 + BBR_RS17935 0.86 -0.5
1,811,668 + BBR_RS17935 0.88 +1.0
1,811,670 + BBR_RS17935 0.88 -2.2
1,811,671 - BBR_RS17935 0.88 -0.9
1,811,671 - BBR_RS17935 0.88 +1.4
1,811,678 - BBR_RS17935 0.88 +2.8
1,811,710 - BBR_RS17935 0.90 -0.4
1,811,756 + -0.6
1,811,803 + +2.5
1,811,811 - +2.6
1,811,813 - +3.6
1,811,835 + -1.5
1,811,835 - -0.6
1,811,848 + -1.6
1,811,873 - -0.6
1,811,921 + -0.6
1,811,964 - -1.5
1,811,964 - -0.4
1,811,965 + -2.2
1,811,973 - +0.7
1,812,028 + -1.9
1,812,028 + +1.5
1,812,035 - +0.1
1,812,035 - +2.5
1,812,036 - -1.1
1,812,036 - -0.5
1,812,036 - +1.7
1,812,096 + BBR_RS17940 0.12 +0.1
1,812,110 + BBR_RS17940 0.15 -1.7
1,812,137 - BBR_RS17940 0.20 +2.8
1,812,144 - BBR_RS17940 0.21 -0.5
1,812,148 - BBR_RS17940 0.22 -2.0
1,812,149 - BBR_RS17940 0.22 +1.1
1,812,177 + BBR_RS17940 0.28 -2.0
1,812,200 - BBR_RS17940 0.32 -1.1
1,812,239 + BBR_RS17940 0.40 +1.0
1,812,242 - BBR_RS17940 0.41 -1.1
1,812,247 - BBR_RS17940 0.42 +0.9
1,812,247 - BBR_RS17940 0.42 -1.2
1,812,282 - BBR_RS17940 0.49 -0.2
1,812,378 + BBR_RS17940 0.67 +2.2
1,812,378 + BBR_RS17940 0.67 -1.5
1,812,378 + BBR_RS17940 0.67 -0.9
1,812,386 - BBR_RS17940 0.69 +2.8
1,812,386 - BBR_RS17940 0.69 -1.6
1,812,386 - BBR_RS17940 0.69 -1.7
1,812,386 - BBR_RS17940 0.69 -2.2
1,812,386 - BBR_RS17940 0.69 -0.9
1,812,449 + BBR_RS17940 0.81 +3.0
1,812,489 + BBR_RS17940 0.89 -0.7
1,812,536 - -2.4
1,812,548 + -2.4
1,812,548 + +0.2
1,812,556 - -0.7
1,812,698 + -2.5
1,812,706 - -0.3
1,812,808 + BBR_RS17945 0.18 +2.3
1,812,852 + BBR_RS17945 0.28 -0.7
1,812,852 + BBR_RS17945 0.28 -1.4
1,812,897 + BBR_RS17945 0.39 -1.8
1,812,920 + BBR_RS17945 0.44 +1.5
1,812,920 + BBR_RS17945 0.44 +0.9
1,812,920 + BBR_RS17945 0.44 -1.4
1,812,928 + BBR_RS17945 0.46 -0.5
1,812,928 + BBR_RS17945 0.46 +3.0
1,812,936 - BBR_RS17945 0.48 -2.0
1,812,936 - BBR_RS17945 0.48 -2.3
1,813,000 - BBR_RS17945 0.63 +1.2
1,813,004 + BBR_RS17945 0.64 -1.0
1,813,037 + BBR_RS17945 0.72 +1.1
1,813,049 + BBR_RS17945 0.74 -1.5
1,813,082 - BBR_RS17945 0.82 -1.3
1,813,114 - BBR_RS17945 0.90 +0.4
1,813,114 - BBR_RS17945 0.90 +3.0
1,813,135 + -0.0
1,813,167 - -2.1
1,813,173 + -2.0
1,813,181 - -1.3
1,813,221 - +0.2
1,813,386 - -0.9
1,813,391 - +0.4
1,813,438 - -1.4
1,813,450 + +1.7
1,813,519 - -0.1
1,813,519 - -0.9
1,813,532 + -0.1

Or see this region's nucleotide sequence