Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS17225

Experiment: reconstituted MRS with Difucosyllactose 7.9 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	reconstituted MRS with Difucosyllactose 7.9 mM
1,641,635	-				-1.6
1,641,643	+				-1.7
1,641,643	+				+1.6
1,641,658	+				-1.1
1,641,666	-				+1.2
1,641,688	+				-1.7
1,641,698	+				-2.2
1,641,718	+				+2.9
1,641,748	+		BBR_RS17220	0.17	-0.7
1,641,750	+		BBR_RS17220	0.17	-1.0
1,641,758	-		BBR_RS17220	0.19	-1.2
1,641,766	+		BBR_RS17220	0.21	+2.3
1,641,777	-		BBR_RS17220	0.24	+0.8
1,641,780	+		BBR_RS17220	0.25	-0.5
1,641,808	-		BBR_RS17220	0.33	+2.9
1,641,869	-		BBR_RS17220	0.49	-1.6
1,641,871	+		BBR_RS17220	0.49	+3.1
1,641,873	+		BBR_RS17220	0.50	-1.4
1,641,904	+		BBR_RS17220	0.58	+1.2
1,641,906	+		BBR_RS17220	0.58	+0.5
1,641,913	-		BBR_RS17220	0.60	+2.1
1,641,967	-		BBR_RS17220	0.75	-1.1
1,641,967	-		BBR_RS17220	0.75	-0.6
1,642,003	-		BBR_RS17220	0.84	+0.7
1,642,025	-				-0.1
1,642,026	+				-1.1
1,642,081	+				-1.0
1,642,086	+				+0.0
1,642,092	+				+0.5
1,642,145	+				+1.7
1,642,166	-				-0.9
1,642,246	+				+0.5
1,642,260	-				-2.3
1,642,306	-				+2.7
1,642,312	+				-1.5
1,642,321	+				+1.9
1,642,321	+				-2.2
1,642,349	-				+2.9
1,642,416	-				+1.2
1,642,436	-				+2.8
1,642,482	+		BBR_RS21000	0.26	-0.3
1,642,518	-		BBR_RS21000	0.41	+2.0
1,642,539	+		BBR_RS21000	0.50	-1.7
1,642,539	+		BBR_RS21000	0.50	+0.1
1,642,602	-				+2.1
1,642,639	+				+0.6
1,642,673	+				+0.0
1,642,675	-				+0.7
1,642,752	+		BBR_RS17225	0.12	-0.8
1,642,774	+		BBR_RS17225	0.14	-1.7
1,642,774	+		BBR_RS17225	0.14	+1.4
1,642,822	+		BBR_RS17225	0.17	+3.0
1,642,822	+		BBR_RS17225	0.17	-0.9
1,642,830	-		BBR_RS17225	0.18	+1.8
1,642,853	-		BBR_RS17225	0.19	-2.3
1,642,903	+		BBR_RS17225	0.23	-1.5
1,642,916	-		BBR_RS17225	0.24	-1.5
1,642,988	+		BBR_RS17225	0.29	+0.8
1,642,988	+		BBR_RS17225	0.29	-2.2
1,642,999	+		BBR_RS17225	0.30	+0.4
1,643,003	+		BBR_RS17225	0.30	-1.8
1,643,097	+		BBR_RS17225	0.37	-2.4
1,643,105	-		BBR_RS17225	0.38	+1.0
1,643,110	+		BBR_RS17225	0.38	+0.4
1,643,129	+		BBR_RS17225	0.39	-1.7
1,643,151	+		BBR_RS17225	0.41	-1.1
1,643,151	+		BBR_RS17225	0.41	-1.0
1,643,151	+		BBR_RS17225	0.41	+0.9
1,643,156	+		BBR_RS17225	0.41	-1.8
1,643,159	+		BBR_RS17225	0.41	+1.2
1,643,159	-		BBR_RS17225	0.41	-0.9
1,643,159	-		BBR_RS17225	0.41	+0.1
1,643,161	-		BBR_RS17225	0.42	-0.4
1,643,169	-		BBR_RS17225	0.42	+3.2
1,643,183	-		BBR_RS17225	0.43	+2.3
1,643,196	-		BBR_RS17225	0.44	-1.2
1,643,206	-		BBR_RS17225	0.45	-2.3
1,643,206	-		BBR_RS17225	0.45	-1.7
1,643,220	-		BBR_RS17225	0.46	-0.5
1,643,236	-		BBR_RS17225	0.47	-1.4
1,643,254	+		BBR_RS17225	0.48	-0.4
1,643,277	+		BBR_RS17225	0.50	-0.9
1,643,285	-		BBR_RS17225	0.51	-1.0
1,643,318	+		BBR_RS17225	0.53	+2.0
1,643,332	+		BBR_RS17225	0.54	+2.3
1,643,332	+		BBR_RS17225	0.54	-0.1
1,643,340	-		BBR_RS17225	0.55	+0.6
1,643,340	-		BBR_RS17225	0.55	-1.4
1,643,340	-		BBR_RS17225	0.55	+1.0
1,643,342	+		BBR_RS17225	0.55	+1.2
1,643,422	-		BBR_RS17225	0.61	+0.5
1,643,453	+		BBR_RS17225	0.63	-1.1
1,643,455	+		BBR_RS17225	0.63	+2.6
1,643,455	-		BBR_RS17225	0.63	-1.4
1,643,492	-		BBR_RS17225	0.66	-0.9
1,643,500	+		BBR_RS17225	0.66	-1.0
1,643,500	+		BBR_RS17225	0.66	-0.2
1,643,500	+		BBR_RS17225	0.66	-1.9
1,643,500	+		BBR_RS17225	0.66	+2.6
1,643,500	+		BBR_RS17225	0.66	-1.5
1,643,500	+		BBR_RS17225	0.66	+1.4
1,643,500	+		BBR_RS17225	0.66	+0.1
1,643,500	+		BBR_RS17225	0.66	-1.2
1,643,500	+		BBR_RS17225	0.66	-0.9
1,643,508	-		BBR_RS17225	0.67	-0.6
1,643,508	-		BBR_RS17225	0.67	+0.5
1,643,508	-		BBR_RS17225	0.67	-0.7
1,643,518	-		BBR_RS17225	0.67	-1.3
1,643,546	-		BBR_RS17225	0.69	-1.1
1,643,580	-		BBR_RS17225	0.72	-0.8
1,643,648	+		BBR_RS17225	0.77	-0.0
1,643,651	-		BBR_RS17225	0.77	+0.3
1,643,666	-		BBR_RS17225	0.78	+0.9
1,643,666	-		BBR_RS17225	0.78	+0.1
1,643,666	-		BBR_RS17225	0.78	+0.7
1,643,671	-		BBR_RS17225	0.79	+2.4
1,643,673	-		BBR_RS17225	0.79	+0.1
1,643,712	-		BBR_RS17225	0.82	+0.7
1,643,739	+		BBR_RS17225	0.83	-1.3
1,643,749	+		BBR_RS17225	0.84	+0.3
1,643,749	+		BBR_RS17225	0.84	-0.4
1,643,749	+		BBR_RS17225	0.84	+1.5
1,643,757	-		BBR_RS17225	0.85	+1.2
1,643,764	+		BBR_RS17225	0.85	+2.5
1,643,786	+		BBR_RS17225	0.87	+1.4
1,643,811	+		BBR_RS17225	0.89	-1.6
1,643,830	+				+2.6
1,643,838	-				+1.4
1,643,838	-				+0.3
1,643,838	-				+1.2
1,643,841	-				-1.8
1,643,845	+				+1.2
1,643,902	+				-2.5
1,643,944	-				+1.5
1,643,981	+				+3.3
1,644,014	+				-1.4
1,644,022	+				-1.0
1,644,022	+				-1.6
1,644,024	+				-1.4
1,644,030	-				-0.7
1,644,085	+				-0.8
1,644,087	+				-0.4
1,644,087	+				-0.9
1,644,100	-				-0.7
1,644,134	-		BBR_RS17230	0.11	+1.9
1,644,162	+		BBR_RS17230	0.13	+1.8
1,644,162	+		BBR_RS17230	0.13	-1.0
1,644,182	+		BBR_RS17230	0.15	+0.1
1,644,223	+		BBR_RS17230	0.17	-0.5
1,644,305	-		BBR_RS17230	0.23	+0.6
1,644,328	-		BBR_RS17230	0.25	-2.1
1,644,345	-		BBR_RS17230	0.26	-1.6
1,644,440	+		BBR_RS17230	0.33	+1.9
1,644,450	+		BBR_RS17230	0.34	-0.9
1,644,486	+		BBR_RS17230	0.36	-1.4
1,644,510	+		BBR_RS17230	0.38	-0.1
1,644,525	+		BBR_RS17230	0.39	-0.2
1,644,533	-		BBR_RS17230	0.39	-2.1
1,644,534	+		BBR_RS17230	0.39	+2.9
1,644,558	-		BBR_RS17230	0.41	+3.6
1,644,563	-		BBR_RS17230	0.42	-1.2
1,644,567	+		BBR_RS17230	0.42	+0.7
1,644,568	+		BBR_RS17230	0.42	+2.7
1,644,630	+		BBR_RS17230	0.46	+2.2
1,644,630	+		BBR_RS17230	0.46	-0.5
1,644,630	+		BBR_RS17230	0.46	+0.1
1,644,638	-		BBR_RS17230	0.47	+0.9
1,644,638	-		BBR_RS17230	0.47	-0.0
1,644,638	-		BBR_RS17230	0.47	-1.5
1,644,640	+		BBR_RS17230	0.47	+0.5
1,644,658	-		BBR_RS17230	0.48	-0.4
1,644,671	-		BBR_RS17230	0.49	-0.3
1,644,762	+		BBR_RS17230	0.56	+0.8
1,644,770	-		BBR_RS17230	0.56	+2.5
1,644,784	-		BBR_RS17230	0.57	+0.5
1,644,794	-		BBR_RS17230	0.58	-0.3
1,644,824	+		BBR_RS17230	0.60	+0.8
1,644,824	+		BBR_RS17230	0.60	-0.2
1,644,860	+		BBR_RS17230	0.63	-0.0
1,644,863	-		BBR_RS17230	0.63	-0.9
1,644,868	+		BBR_RS17230	0.63	+3.6
1,644,914	+		BBR_RS17230	0.66	+1.5
1,644,927	+		BBR_RS17230	0.67	+2.2
1,644,935	-		BBR_RS17230	0.68	-0.4
1,644,935	-		BBR_RS17230	0.68	-1.2
1,644,935	-		BBR_RS17230	0.68	-1.1
1,644,937	+		BBR_RS17230	0.68	+2.0
1,644,937	+		BBR_RS17230	0.68	-1.2
1,644,937	+		BBR_RS17230	0.68	+0.1
1,644,945	-		BBR_RS17230	0.69	+2.6
1,644,964	+		BBR_RS17230	0.70	+1.0