Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS16840

Experiment: reconstituted MRS with Difucosyllactose 7.9 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	reconstituted MRS with Difucosyllactose 7.9 mM
1,545,707	+				+2.0
1,545,830	-				-1.5
1,545,830	-				+3.7
1,545,878	-				-1.0
1,545,879	-				+1.0
1,545,932	+		BBR_RS16835	0.11	+2.1
1,545,940	-		BBR_RS16835	0.13	-1.9
1,545,949	+		BBR_RS16835	0.15	-0.3
1,545,987	+		BBR_RS16835	0.23	-0.6
1,545,992	+		BBR_RS16835	0.24	+0.0
1,546,041	+		BBR_RS16835	0.35	+2.1
1,546,070	-		BBR_RS16835	0.41	-0.3
1,546,103	+		BBR_RS16835	0.49	+3.5
1,546,132	+		BBR_RS16835	0.55	+3.8
1,546,167	+		BBR_RS16835	0.63	+0.1
1,546,204	-		BBR_RS16835	0.71	+0.2
1,546,216	+		BBR_RS16835	0.73	+2.6
1,546,223	+		BBR_RS16835	0.75	+2.3
1,546,301	+				+1.8
1,546,311	+				-0.5
1,546,319	-				+0.2
1,546,460	+				+0.4
1,546,484	-				+1.0
1,546,520	+				+1.6
1,546,551	+				-1.1
1,546,561	+				-1.2
1,546,634	-				-0.5
1,546,649	-				-1.2
1,546,699	+				-0.9
1,546,699	+				+0.2
1,546,707	-				-0.7
1,546,709	-				+2.5
1,546,725	-				+0.4
1,546,749	-		BBR_RS16840	0.10	-1.1
1,546,760	-		BBR_RS16840	0.11	-0.1
1,546,809	+		BBR_RS16840	0.12	-2.3
1,546,817	-		BBR_RS16840	0.12	-1.2
1,546,826	-		BBR_RS16840	0.12	-0.1
1,546,892	-		BBR_RS16840	0.14	+0.0
1,546,934	-		BBR_RS16840	0.15	+0.1
1,546,950	+		BBR_RS16840	0.16	-0.2
1,546,994	-		BBR_RS16840	0.17	-0.9
1,547,031	+		BBR_RS16840	0.18	-0.6
1,547,048	-		BBR_RS16840	0.19	+0.4
1,547,072	+		BBR_RS16840	0.19	+2.0
1,547,077	+		BBR_RS16840	0.19	-0.3
1,547,120	+		BBR_RS16840	0.21	+4.2
1,547,126	+		BBR_RS16840	0.21	+0.7
1,547,150	-		BBR_RS16840	0.21	+3.0
1,547,217	-		BBR_RS16840	0.23	-2.6
1,547,217	-		BBR_RS16840	0.23	-0.8
1,547,251	-		BBR_RS16840	0.24	-2.3
1,547,281	+		BBR_RS16840	0.25	+2.5
1,547,360	-		BBR_RS16840	0.27	-0.3
1,547,384	+		BBR_RS16840	0.28	+2.3
1,547,394	-		BBR_RS16840	0.28	+1.2
1,547,414	+		BBR_RS16840	0.29	+1.6
1,547,538	-		BBR_RS16840	0.32	-0.6
1,547,579	-		BBR_RS16840	0.33	-0.6
1,547,590	+		BBR_RS16840	0.33	-0.8
1,547,637	-		BBR_RS16840	0.35	-2.2
1,547,655	-		BBR_RS16840	0.35	-0.4
1,547,655	-		BBR_RS16840	0.35	-0.6
1,547,669	+		BBR_RS16840	0.36	+0.2
1,547,680	+		BBR_RS16840	0.36	+1.9
1,547,710	-		BBR_RS16840	0.37	-1.2
1,547,776	+		BBR_RS16840	0.39	-1.7
1,547,833	+		BBR_RS16840	0.40	-1.7
1,547,833	+		BBR_RS16840	0.40	-1.3
1,547,838	+		BBR_RS16840	0.40	-1.4
1,547,841	-		BBR_RS16840	0.40	-0.7
1,547,841	-		BBR_RS16840	0.40	-0.4
1,547,841	-		BBR_RS16840	0.40	-0.6
1,547,846	-		BBR_RS16840	0.40	+3.9
1,547,903	-		BBR_RS16840	0.42	-1.3
1,547,999	+		BBR_RS16840	0.45	-2.2
1,548,064	+		BBR_RS16840	0.46	+3.0
1,548,081	+		BBR_RS16840	0.47	-0.6
1,548,098	+		BBR_RS16840	0.47	+0.4
1,548,117	-		BBR_RS16840	0.48	+1.2
1,548,130	-		BBR_RS16840	0.48	-0.3
1,548,173	-		BBR_RS16840	0.49	-0.1
1,548,187	-		BBR_RS16840	0.50	-0.0
1,548,199	+		BBR_RS16840	0.50	-0.5
1,548,210	-		BBR_RS16840	0.50	-0.8
1,548,214	+		BBR_RS16840	0.51	+0.2
1,548,218	+		BBR_RS16840	0.51	+0.1
1,548,227	-		BBR_RS16840	0.51	+2.8
1,548,257	-		BBR_RS16840	0.52	-2.1
1,548,283	-		BBR_RS16840	0.52	-2.1
1,548,361	-		BBR_RS16840	0.55	-0.0
1,548,542	+		BBR_RS16840	0.60	+2.9
1,548,551	+		BBR_RS16840	0.60	-1.8
1,548,557	-		BBR_RS16840	0.60	+1.0
1,548,559	+		BBR_RS16840	0.60	-1.0
1,548,559	-		BBR_RS16840	0.60	-1.0
1,548,567	+		BBR_RS16840	0.60	+0.4
1,548,567	+		BBR_RS16840	0.60	-1.5
1,548,575	-		BBR_RS16840	0.60	+1.3
1,548,596	-		BBR_RS16840	0.61	-1.8
1,548,596	-		BBR_RS16840	0.61	-1.2
1,548,596	-		BBR_RS16840	0.61	+1.2
1,548,596	-		BBR_RS16840	0.61	-2.0
1,548,604	-		BBR_RS16840	0.61	-1.7
1,548,626	+		BBR_RS16840	0.62	-0.9
1,548,642	-		BBR_RS16840	0.62	-2.3
1,548,642	-		BBR_RS16840	0.62	-0.5
1,548,642	-		BBR_RS16840	0.62	-0.6
1,548,642	-		BBR_RS16840	0.62	-0.7
1,548,654	-		BBR_RS16840	0.63	-0.4
1,548,663	+		BBR_RS16840	0.63	+1.5
1,548,777	+		BBR_RS16840	0.66	-0.5
1,548,876	+		BBR_RS16840	0.69	+0.4
1,548,884	-		BBR_RS16840	0.69	-0.2
1,548,896	+		BBR_RS16840	0.69	+3.2
1,548,896	+		BBR_RS16840	0.69	-0.7
1,548,896	+		BBR_RS16840	0.69	-0.8
1,548,958	-		BBR_RS16840	0.71	+1.3
1,548,989	-		BBR_RS16840	0.72	+0.1
1,548,989	-		BBR_RS16840	0.72	+2.2
1,548,989	-		BBR_RS16840	0.72	+0.2
1,549,007	+		BBR_RS16840	0.72	-1.7
1,549,023	+		BBR_RS16840	0.73	-0.3
1,549,105	+		BBR_RS16840	0.75	-0.6
1,549,113	-		BBR_RS16840	0.75	+0.4
1,549,218	+		BBR_RS16840	0.78	-2.8
1,549,220	-		BBR_RS16840	0.78	+2.6
1,549,225	-		BBR_RS16840	0.78	-1.2
1,549,226	-		BBR_RS16840	0.78	-1.0
1,549,226	-		BBR_RS16840	0.78	+1.6
1,549,226	-		BBR_RS16840	0.78	+0.0
1,549,226	-		BBR_RS16840	0.78	-1.3
1,549,228	+		BBR_RS16840	0.78	-0.7
1,549,228	+		BBR_RS16840	0.78	-0.0
1,549,251	-		BBR_RS16840	0.79	+3.2
1,549,253	+		BBR_RS16840	0.79	+0.4
1,549,321	+		BBR_RS16840	0.81	-0.8
1,549,347	-		BBR_RS16840	0.82	-1.1
1,549,361	-		BBR_RS16840	0.82	-0.8
1,549,475	-		BBR_RS16840	0.85	+1.8
1,549,484	-		BBR_RS16840	0.85	+0.2
1,549,508	-		BBR_RS16840	0.86	+2.4
1,549,508	-		BBR_RS16840	0.86	-0.6
1,549,510	+		BBR_RS16840	0.86	-0.2
1,549,558	+		BBR_RS16840	0.87	+4.0
1,549,790	+				-0.5
1,549,790	+				+0.8
1,549,792	+				+0.6
1,549,798	-				+1.3
1,549,798	-				+3.0
1,549,800	-				-0.7
1,549,842	-				+3.4
1,549,842	-				+0.6
1,549,853	-				+4.3
1,549,865	+				-1.0
1,549,865	+				+2.6
1,549,878	+				+0.0
1,549,886	-				-0.3
1,549,886	-				+1.7
1,549,944	-				+2.5
1,550,026	-				+1.8
1,550,042	+				-1.1
1,550,042	+				-0.3
1,550,050	-				-0.9
1,550,063	-				+1.3
1,550,081	+				+0.3
1,550,094	-				-1.9
1,550,094	-				+1.4
1,550,124	+				-1.0
1,550,130	+				-1.4
1,550,168	+				+0.4
1,550,176	-				-0.0
1,550,195	+				+1.3
1,550,213	-				-1.3
1,550,258	+		BBR_RS16845	0.12	+0.2
1,550,258	+		BBR_RS16845	0.12	+0.4
1,550,274	+		BBR_RS16845	0.13	+3.7
1,550,279	+		BBR_RS16845	0.14	+1.4