Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS15535

Experiment: reconstituted MRS with Difucosyllactose 7.9 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS15530 and BBR_RS15535 are separated by 158 nucleotidesBBR_RS15535 and BBR_RS15540 are separated by 150 nucleotides BBR_RS15530: BBR_RS15530 - DNA-binding protein WhiA, at 1,258,273 to 1,259,223 _RS15530 BBR_RS15535: BBR_RS15535 - phosphoglycerate kinase, at 1,259,382 to 1,260,587 _RS15535 BBR_RS15540: BBR_RS15540 - triose-phosphate isomerase, at 1,260,738 to 1,261,541 _RS15540 Position (kb) 1259 1260 1261Strain fitness (log2 ratio) -2 -1 0 1 2at 1259.300 kb on + strandat 1259.323 kb on - strandat 1259.324 kb on + strandat 1260.635 kb on + strandat 1260.700 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM
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1,259,300 + +1.5
1,259,323 - +2.1
1,259,324 + -2.5
1,260,635 + +0.1
1,260,700 - +0.6

Or see this region's nucleotide sequence