Experiment: reconstituted MRS with Difucosyllactose 7.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15380 and BBR_RS15385 are separated by 12 nucleotides BBR_RS15385 and BBR_RS15390 are separated by 428 nucleotides
BBR_RS15380: BBR_RS15380 - thiaminase II, at 1,220,580 to 1,221,317
_RS15380
BBR_RS15385: BBR_RS15385 - hypothetical protein, at 1,221,330 to 1,221,626
_RS15385
BBR_RS15390: BBR_RS15390 - phospholipase, at 1,222,055 to 1,222,798
_RS15390
Position (kb)
1221
1222 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1220.348 kb on - strand at 1220.376 kb on - strand at 1220.402 kb on - strand at 1220.448 kb on - strand at 1220.471 kb on + strand at 1220.542 kb on - strand at 1220.542 kb on - strand at 1220.572 kb on - strand at 1220.616 kb on + strand at 1220.617 kb on + strand at 1220.617 kb on + strand at 1220.625 kb on - strand at 1220.641 kb on + strand at 1220.736 kb on - strand, within BBR_RS15380 at 1220.759 kb on + strand, within BBR_RS15380 at 1220.762 kb on - strand, within BBR_RS15380 at 1220.767 kb on - strand, within BBR_RS15380 at 1220.797 kb on - strand, within BBR_RS15380 at 1220.807 kb on + strand, within BBR_RS15380 at 1220.829 kb on + strand, within BBR_RS15380 at 1220.830 kb on - strand, within BBR_RS15380 at 1220.845 kb on - strand, within BBR_RS15380 at 1220.886 kb on - strand, within BBR_RS15380 at 1220.943 kb on - strand, within BBR_RS15380 at 1220.968 kb on - strand, within BBR_RS15380 at 1220.978 kb on + strand, within BBR_RS15380 at 1221.034 kb on + strand, within BBR_RS15380 at 1221.037 kb on - strand, within BBR_RS15380 at 1221.041 kb on + strand, within BBR_RS15380 at 1221.042 kb on - strand, within BBR_RS15380 at 1221.042 kb on - strand, within BBR_RS15380 at 1221.042 kb on - strand, within BBR_RS15380 at 1221.110 kb on - strand, within BBR_RS15380 at 1221.125 kb on + strand, within BBR_RS15380 at 1221.133 kb on - strand, within BBR_RS15380 at 1221.140 kb on + strand, within BBR_RS15380 at 1221.154 kb on + strand, within BBR_RS15380 at 1221.154 kb on + strand, within BBR_RS15380 at 1221.159 kb on - strand, within BBR_RS15380 at 1221.159 kb on - strand, within BBR_RS15380 at 1221.192 kb on + strand, within BBR_RS15380 at 1221.255 kb on + strand at 1221.265 kb on + strand at 1221.281 kb on - strand at 1221.290 kb on - strand at 1221.292 kb on - strand at 1221.328 kb on + strand at 1221.329 kb on + strand at 1221.364 kb on + strand, within BBR_RS15385 at 1221.406 kb on + strand, within BBR_RS15385 at 1221.406 kb on + strand, within BBR_RS15385 at 1221.414 kb on - strand, within BBR_RS15385 at 1221.414 kb on - strand, within BBR_RS15385 at 1221.452 kb on + strand, within BBR_RS15385 at 1221.541 kb on + strand, within BBR_RS15385 at 1221.550 kb on - strand, within BBR_RS15385 at 1221.563 kb on + strand, within BBR_RS15385 at 1221.771 kb on - strand at 1221.781 kb on + strand at 1221.781 kb on + strand at 1221.781 kb on + strand at 1221.781 kb on + strand at 1221.781 kb on + strand at 1221.781 kb on + strand at 1221.789 kb on - strand at 1221.794 kb on - strand at 1221.873 kb on - strand at 1221.909 kb on + strand at 1221.924 kb on + strand at 1221.953 kb on + strand at 1221.970 kb on - strand at 1221.974 kb on + strand at 1222.010 kb on - strand at 1222.024 kb on - strand at 1222.136 kb on + strand, within BBR_RS15390 at 1222.144 kb on - strand, within BBR_RS15390 at 1222.144 kb on - strand, within BBR_RS15390 at 1222.168 kb on - strand, within BBR_RS15390 at 1222.220 kb on - strand, within BBR_RS15390 at 1222.220 kb on - strand, within BBR_RS15390 at 1222.249 kb on - strand, within BBR_RS15390 at 1222.280 kb on + strand, within BBR_RS15390 at 1222.305 kb on + strand, within BBR_RS15390 at 1222.314 kb on + strand, within BBR_RS15390 at 1222.334 kb on - strand, within BBR_RS15390 at 1222.411 kb on + strand, within BBR_RS15390 at 1222.423 kb on + strand, within BBR_RS15390 at 1222.488 kb on + strand, within BBR_RS15390 at 1222.535 kb on + strand, within BBR_RS15390
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM remove 1,220,348 - -0.6 1,220,376 - -0.7 1,220,402 - +0.9 1,220,448 - -1.3 1,220,471 + -1.9 1,220,542 - -0.1 1,220,542 - -0.4 1,220,572 - +3.4 1,220,616 + -1.0 1,220,617 + -1.2 1,220,617 + +2.6 1,220,625 - +2.3 1,220,641 + -1.0 1,220,736 - BBR_RS15380 0.21 +1.4 1,220,759 + BBR_RS15380 0.24 +0.3 1,220,762 - BBR_RS15380 0.25 +1.3 1,220,767 - BBR_RS15380 0.25 -1.4 1,220,797 - BBR_RS15380 0.29 +1.7 1,220,807 + BBR_RS15380 0.31 +2.7 1,220,829 + BBR_RS15380 0.34 -2.0 1,220,830 - BBR_RS15380 0.34 -1.7 1,220,845 - BBR_RS15380 0.36 +1.4 1,220,886 - BBR_RS15380 0.41 -0.1 1,220,943 - BBR_RS15380 0.49 -0.3 1,220,968 - BBR_RS15380 0.53 -1.3 1,220,978 + BBR_RS15380 0.54 +0.3 1,221,034 + BBR_RS15380 0.62 +1.1 1,221,037 - BBR_RS15380 0.62 -1.6 1,221,041 + BBR_RS15380 0.62 -0.5 1,221,042 - BBR_RS15380 0.63 -1.0 1,221,042 - BBR_RS15380 0.63 -0.5 1,221,042 - BBR_RS15380 0.63 +0.1 1,221,110 - BBR_RS15380 0.72 +1.8 1,221,125 + BBR_RS15380 0.74 -1.0 1,221,133 - BBR_RS15380 0.75 -0.5 1,221,140 + BBR_RS15380 0.76 -1.3 1,221,154 + BBR_RS15380 0.78 +2.9 1,221,154 + BBR_RS15380 0.78 -0.6 1,221,159 - BBR_RS15380 0.78 +0.5 1,221,159 - BBR_RS15380 0.78 -0.2 1,221,192 + BBR_RS15380 0.83 +3.8 1,221,255 + -0.5 1,221,265 + +0.4 1,221,281 - -1.2 1,221,290 - -1.4 1,221,292 - -1.5 1,221,328 + +3.5 1,221,329 + +1.2 1,221,364 + BBR_RS15385 0.11 -1.0 1,221,406 + BBR_RS15385 0.26 -1.2 1,221,406 + BBR_RS15385 0.26 -0.3 1,221,414 - BBR_RS15385 0.28 -0.6 1,221,414 - BBR_RS15385 0.28 +1.0 1,221,452 + BBR_RS15385 0.41 -1.6 1,221,541 + BBR_RS15385 0.71 +1.3 1,221,550 - BBR_RS15385 0.74 -0.1 1,221,563 + BBR_RS15385 0.78 +2.2 1,221,771 - -1.6 1,221,781 + +2.7 1,221,781 + -1.2 1,221,781 + +2.3 1,221,781 + -0.8 1,221,781 + +1.4 1,221,781 + -0.2 1,221,789 - -1.6 1,221,794 - +1.5 1,221,873 - +0.9 1,221,909 + -1.7 1,221,924 + +0.9 1,221,953 + +0.1 1,221,970 - +0.0 1,221,974 + +2.2 1,222,010 - -1.1 1,222,024 - -0.3 1,222,136 + BBR_RS15390 0.11 +2.4 1,222,144 - BBR_RS15390 0.12 +0.5 1,222,144 - BBR_RS15390 0.12 -0.8 1,222,168 - BBR_RS15390 0.15 +3.2 1,222,220 - BBR_RS15390 0.22 +3.6 1,222,220 - BBR_RS15390 0.22 -0.1 1,222,249 - BBR_RS15390 0.26 +3.2 1,222,280 + BBR_RS15390 0.30 +0.8 1,222,305 + BBR_RS15390 0.34 +0.4 1,222,314 + BBR_RS15390 0.35 +3.1 1,222,334 - BBR_RS15390 0.38 -0.3 1,222,411 + BBR_RS15390 0.48 -1.4 1,222,423 + BBR_RS15390 0.49 +2.4 1,222,488 + BBR_RS15390 0.58 -1.0 1,222,535 + BBR_RS15390 0.65 -1.3
Or see this region's nucleotide sequence