Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS15320

Experiment: reconstituted MRS with Difucosyllactose 7.9 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS15310 and BBR_RS15315 are separated by 2 nucleotidesBBR_RS15315 and BBR_RS15320 are separated by 105 nucleotidesBBR_RS15320 and BBR_RS15325 are separated by 216 nucleotidesBBR_RS15325 and BBR_RS15330 are separated by 10 nucleotidesBBR_RS15330 and BBR_RS15335 are separated by 24 nucleotides BBR_RS15310: BBR_RS15310 - IS21 family transposase, at 1,208,978 to 1,210,459 _RS15310 BBR_RS15315: BBR_RS15315 - ATPase AAA, at 1,210,462 to 1,211,247 _RS15315 BBR_RS15320: BBR_RS15320 - hypothetical protein, at 1,211,353 to 1,211,622 _RS15320 BBR_RS15325: BBR_RS15325 - hypothetical protein, at 1,211,839 to 1,212,024 _RS15325 BBR_RS15330: BBR_RS15330 - hypothetical protein, at 1,212,035 to 1,212,556 _RS15330 BBR_RS15335: BBR_RS15335 - hypothetical protein, at 1,212,581 to 1,213,315 _RS15335 Position (kb) 1211 1212Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 1211.380 kb on - strand, within BBR_RS15320at 1211.504 kb on - strand, within BBR_RS15320at 1211.504 kb on - strand, within BBR_RS15320at 1211.510 kb on - strand, within BBR_RS15320at 1211.544 kb on + strand, within BBR_RS15320at 1211.552 kb on - strand, within BBR_RS15320at 1211.613 kb on + strandat 1211.632 kb on + strandat 1211.667 kb on + strandat 1211.777 kb on - strandat 1211.845 kb on - strandat 1211.845 kb on - strandat 1211.890 kb on + strand, within BBR_RS15325at 1211.912 kb on - strand, within BBR_RS15325at 1211.943 kb on + strand, within BBR_RS15325at 1211.943 kb on + strand, within BBR_RS15325at 1211.965 kb on - strand, within BBR_RS15325at 1211.983 kb on - strand, within BBR_RS15325at 1211.983 kb on - strand, within BBR_RS15325at 1211.996 kb on - strand, within BBR_RS15325at 1212.041 kb on + strandat 1212.079 kb on + strandat 1212.079 kb on + strandat 1212.079 kb on + strandat 1212.180 kb on - strand, within BBR_RS15330at 1212.180 kb on - strand, within BBR_RS15330at 1212.226 kb on - strand, within BBR_RS15330at 1212.311 kb on + strand, within BBR_RS15330at 1212.338 kb on - strand, within BBR_RS15330at 1212.346 kb on - strand, within BBR_RS15330at 1212.553 kb on + strandat 1212.554 kb on + strandat 1212.562 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM
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1,211,380 - BBR_RS15320 0.10 +1.1
1,211,504 - BBR_RS15320 0.56 +0.3
1,211,504 - BBR_RS15320 0.56 +1.2
1,211,510 - BBR_RS15320 0.58 -1.7
1,211,544 + BBR_RS15320 0.71 -1.6
1,211,552 - BBR_RS15320 0.74 -0.8
1,211,613 + -0.8
1,211,632 + +0.2
1,211,667 + -2.4
1,211,777 - +0.6
1,211,845 - +2.0
1,211,845 - +5.0
1,211,890 + BBR_RS15325 0.27 +0.6
1,211,912 - BBR_RS15325 0.39 -0.9
1,211,943 + BBR_RS15325 0.56 +0.3
1,211,943 + BBR_RS15325 0.56 +2.1
1,211,965 - BBR_RS15325 0.68 -1.3
1,211,983 - BBR_RS15325 0.77 -0.4
1,211,983 - BBR_RS15325 0.77 +1.8
1,211,996 - BBR_RS15325 0.84 +0.1
1,212,041 + +2.2
1,212,079 + +3.1
1,212,079 + -0.1
1,212,079 + +2.0
1,212,180 - BBR_RS15330 0.28 +2.2
1,212,180 - BBR_RS15330 0.28 +0.8
1,212,226 - BBR_RS15330 0.37 +1.8
1,212,311 + BBR_RS15330 0.53 +1.2
1,212,338 - BBR_RS15330 0.58 +2.2
1,212,346 - BBR_RS15330 0.60 -0.1
1,212,553 + -1.1
1,212,554 + -1.2
1,212,562 - +4.3

Or see this region's nucleotide sequence