Experiment: reconstituted MRS with Difucosyllactose 7.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15020 and BBR_RS15025 are separated by 104 nucleotides BBR_RS15025 and BBR_RS15030 are separated by 176 nucleotides BBR_RS15030 and BBR_RS15035 overlap by 14 nucleotides BBR_RS15035 and BBR_RS15040 are separated by 150 nucleotides BBR_RS15040 and BBR_RS15045 are separated by 75 nucleotides
BBR_RS15020: BBR_RS15020 - membrane protein, at 1,142,575 to 1,142,931
_RS15020
BBR_RS15025: BBR_RS15025 - transglutaminase family protein, at 1,143,036 to 1,143,638
_RS15025
BBR_RS15030: BBR_RS15030 - mRNA interferase PemK, at 1,143,815 to 1,144,171
_RS15030
BBR_RS15035: BBR_RS15035 - translation repressor RelB, at 1,144,158 to 1,144,451
_RS15035
BBR_RS15040: BBR_RS15040 - hypothetical protein, at 1,144,602 to 1,144,790
_RS15040
BBR_RS15045: BBR_RS15045 - histidine phosphatase family protein, at 1,144,866 to 1,145,540
_RS15045
Position (kb)
1143
1144
1145 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1142.897 kb on + strand at 1142.928 kb on - strand at 1143.031 kb on + strand at 1143.116 kb on - strand, within BBR_RS15025 at 1143.213 kb on + strand, within BBR_RS15025 at 1143.250 kb on + strand, within BBR_RS15025 at 1143.250 kb on + strand, within BBR_RS15025 at 1143.325 kb on - strand, within BBR_RS15025 at 1143.331 kb on + strand, within BBR_RS15025 at 1143.439 kb on + strand, within BBR_RS15025 at 1143.448 kb on + strand, within BBR_RS15025 at 1143.449 kb on + strand, within BBR_RS15025 at 1143.780 kb on + strand at 1143.782 kb on + strand at 1143.785 kb on + strand at 1143.818 kb on + strand at 1143.819 kb on - strand at 1143.857 kb on - strand, within BBR_RS15030 at 1143.934 kb on + strand, within BBR_RS15030 at 1143.951 kb on + strand, within BBR_RS15030 at 1143.951 kb on + strand, within BBR_RS15030 at 1143.951 kb on + strand, within BBR_RS15030 at 1143.959 kb on - strand, within BBR_RS15030 at 1143.959 kb on - strand, within BBR_RS15030 at 1143.991 kb on + strand, within BBR_RS15030 at 1143.999 kb on - strand, within BBR_RS15030 at 1144.041 kb on - strand, within BBR_RS15030 at 1144.094 kb on - strand, within BBR_RS15030 at 1144.099 kb on - strand, within BBR_RS15030 at 1144.099 kb on - strand, within BBR_RS15030 at 1144.459 kb on + strand at 1144.459 kb on - strand at 1144.467 kb on - strand at 1144.793 kb on - strand at 1144.793 kb on - strand at 1144.872 kb on - strand at 1144.924 kb on - strand at 1144.929 kb on - strand at 1144.945 kb on - strand, within BBR_RS15045 at 1144.971 kb on + strand, within BBR_RS15045 at 1144.992 kb on + strand, within BBR_RS15045 at 1145.001 kb on + strand, within BBR_RS15045 at 1145.115 kb on + strand, within BBR_RS15045 at 1145.155 kb on + strand, within BBR_RS15045 at 1145.155 kb on - strand, within BBR_RS15045 at 1145.171 kb on + strand, within BBR_RS15045 at 1145.171 kb on + strand, within BBR_RS15045 at 1145.171 kb on + strand, within BBR_RS15045 at 1145.171 kb on + strand, within BBR_RS15045
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM remove 1,142,897 + -0.7 1,142,928 - +0.8 1,143,031 + -1.5 1,143,116 - BBR_RS15025 0.13 +0.2 1,143,213 + BBR_RS15025 0.29 +0.2 1,143,250 + BBR_RS15025 0.35 +1.6 1,143,250 + BBR_RS15025 0.35 +0.0 1,143,325 - BBR_RS15025 0.48 +0.8 1,143,331 + BBR_RS15025 0.49 -1.9 1,143,439 + BBR_RS15025 0.67 -0.6 1,143,448 + BBR_RS15025 0.68 -1.1 1,143,449 + BBR_RS15025 0.68 -1.3 1,143,780 + +1.2 1,143,782 + +3.5 1,143,785 + +1.6 1,143,818 + +4.8 1,143,819 - -0.7 1,143,857 - BBR_RS15030 0.12 -0.8 1,143,934 + BBR_RS15030 0.33 +4.0 1,143,951 + BBR_RS15030 0.38 -0.1 1,143,951 + BBR_RS15030 0.38 -0.6 1,143,951 + BBR_RS15030 0.38 +2.5 1,143,959 - BBR_RS15030 0.40 -0.9 1,143,959 - BBR_RS15030 0.40 +2.5 1,143,991 + BBR_RS15030 0.49 -0.7 1,143,999 - BBR_RS15030 0.52 +3.1 1,144,041 - BBR_RS15030 0.63 +0.1 1,144,094 - BBR_RS15030 0.78 +0.4 1,144,099 - BBR_RS15030 0.80 +5.2 1,144,099 - BBR_RS15030 0.80 +2.9 1,144,459 + +3.5 1,144,459 - -0.3 1,144,467 - -0.1 1,144,793 - -1.5 1,144,793 - -1.5 1,144,872 - +2.6 1,144,924 - -0.8 1,144,929 - -1.7 1,144,945 - BBR_RS15045 0.12 -0.4 1,144,971 + BBR_RS15045 0.16 -1.7 1,144,992 + BBR_RS15045 0.19 -0.3 1,145,001 + BBR_RS15045 0.20 -1.5 1,145,115 + BBR_RS15045 0.37 -1.5 1,145,155 + BBR_RS15045 0.43 -1.8 1,145,155 - BBR_RS15045 0.43 +3.0 1,145,171 + BBR_RS15045 0.45 -0.3 1,145,171 + BBR_RS15045 0.45 +1.3 1,145,171 + BBR_RS15045 0.45 +1.3 1,145,171 + BBR_RS15045 0.45 +3.2
Or see this region's nucleotide sequence