Experiment: reconstituted MRS with Difucosyllactose 7.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14140 and BBR_RS14145 are separated by 33 nucleotides BBR_RS14145 and BBR_RS14150 are separated by 126 nucleotides BBR_RS14150 and BBR_RS14155 are separated by 131 nucleotides
BBR_RS14140: BBR_RS14140 - Ppx/GppA family phosphatase, at 949,005 to 950,006
_RS14140
BBR_RS14145: BBR_RS14145 - tRNA-Leu, at 950,040 to 950,119
_RS14145
BBR_RS14150: BBR_RS14150 - hypothetical protein, at 950,246 to 950,815
_RS14150
BBR_RS14155: BBR_RS14155 - L-serine ammonia-lyase, at 950,947 to 952,404
_RS14155
Position (kb) 950
951 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7 at 949.470 kb on + strand, within BBR_RS14140 at 949.581 kb on + strand, within BBR_RS14140 at 949.630 kb on - strand, within BBR_RS14140 at 949.667 kb on + strand, within BBR_RS14140 at 949.777 kb on + strand, within BBR_RS14140 at 949.931 kb on - strand at 949.961 kb on + strand at 950.007 kb on - strand at 950.009 kb on - strand at 950.015 kb on + strand at 950.192 kb on + strand at 950.202 kb on + strand at 950.202 kb on + strand at 950.210 kb on - strand at 950.242 kb on + strand at 950.296 kb on - strand at 950.361 kb on + strand, within BBR_RS14150 at 950.428 kb on - strand, within BBR_RS14150 at 950.505 kb on + strand, within BBR_RS14150 at 950.518 kb on + strand, within BBR_RS14150 at 950.519 kb on + strand, within BBR_RS14150 at 950.545 kb on - strand, within BBR_RS14150 at 950.610 kb on + strand, within BBR_RS14150 at 950.652 kb on + strand, within BBR_RS14150 at 950.722 kb on + strand, within BBR_RS14150 at 950.723 kb on + strand, within BBR_RS14150 at 950.730 kb on + strand, within BBR_RS14150 at 950.738 kb on - strand, within BBR_RS14150 at 950.754 kb on - strand, within BBR_RS14150 at 950.878 kb on + strand at 950.893 kb on + strand at 950.944 kb on + strand at 950.946 kb on + strand at 950.952 kb on - strand at 950.952 kb on - strand at 951.010 kb on - strand at 951.010 kb on - strand at 951.093 kb on + strand, within BBR_RS14155 at 951.150 kb on - strand, within BBR_RS14155 at 951.166 kb on + strand, within BBR_RS14155 at 951.174 kb on - strand, within BBR_RS14155 at 951.176 kb on + strand, within BBR_RS14155 at 951.177 kb on + strand, within BBR_RS14155 at 951.222 kb on + strand, within BBR_RS14155 at 951.246 kb on + strand, within BBR_RS14155 at 951.254 kb on - strand, within BBR_RS14155 at 951.308 kb on - strand, within BBR_RS14155 at 951.357 kb on - strand, within BBR_RS14155 at 951.404 kb on + strand, within BBR_RS14155 at 951.451 kb on - strand, within BBR_RS14155 at 951.462 kb on + strand, within BBR_RS14155 at 951.462 kb on + strand, within BBR_RS14155 at 951.481 kb on - strand, within BBR_RS14155 at 951.510 kb on + strand, within BBR_RS14155 at 951.518 kb on - strand, within BBR_RS14155 at 951.621 kb on + strand, within BBR_RS14155 at 951.621 kb on + strand, within BBR_RS14155 at 951.659 kb on - strand, within BBR_RS14155 at 951.678 kb on - strand, within BBR_RS14155 at 951.697 kb on + strand, within BBR_RS14155 at 951.737 kb on + strand, within BBR_RS14155 at 951.751 kb on + strand, within BBR_RS14155 at 951.755 kb on - strand, within BBR_RS14155 at 951.796 kb on - strand, within BBR_RS14155
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM remove 949,470 + BBR_RS14140 0.46 +0.8 949,581 + BBR_RS14140 0.57 +2.1 949,630 - BBR_RS14140 0.62 -0.5 949,667 + BBR_RS14140 0.66 +7.7 949,777 + BBR_RS14140 0.77 -0.6 949,931 - +0.9 949,961 + +2.4 950,007 - +0.5 950,009 - +4.3 950,015 + -1.5 950,192 + -1.0 950,202 + -1.3 950,202 + +0.9 950,210 - +0.5 950,242 + +2.4 950,296 - -0.4 950,361 + BBR_RS14150 0.20 -1.8 950,428 - BBR_RS14150 0.32 +0.2 950,505 + BBR_RS14150 0.45 -1.0 950,518 + BBR_RS14150 0.48 -2.2 950,519 + BBR_RS14150 0.48 -0.4 950,545 - BBR_RS14150 0.52 -0.1 950,610 + BBR_RS14150 0.64 +3.4 950,652 + BBR_RS14150 0.71 +1.0 950,722 + BBR_RS14150 0.84 -0.9 950,723 + BBR_RS14150 0.84 -1.7 950,730 + BBR_RS14150 0.85 -1.2 950,738 - BBR_RS14150 0.86 -0.5 950,754 - BBR_RS14150 0.89 -0.8 950,878 + -1.2 950,893 + -1.4 950,944 + -1.4 950,946 + -2.1 950,952 - +3.3 950,952 - -1.7 951,010 - -0.2 951,010 - -0.4 951,093 + BBR_RS14155 0.10 -1.2 951,150 - BBR_RS14155 0.14 -0.1 951,166 + BBR_RS14155 0.15 +0.3 951,174 - BBR_RS14155 0.16 +0.4 951,176 + BBR_RS14155 0.16 +0.2 951,177 + BBR_RS14155 0.16 +0.4 951,222 + BBR_RS14155 0.19 +0.2 951,246 + BBR_RS14155 0.21 +0.6 951,254 - BBR_RS14155 0.21 -1.0 951,308 - BBR_RS14155 0.25 -2.3 951,357 - BBR_RS14155 0.28 +1.7 951,404 + BBR_RS14155 0.31 +1.8 951,451 - BBR_RS14155 0.35 +1.0 951,462 + BBR_RS14155 0.35 -0.8 951,462 + BBR_RS14155 0.35 -0.0 951,481 - BBR_RS14155 0.37 -0.6 951,510 + BBR_RS14155 0.39 +2.5 951,518 - BBR_RS14155 0.39 +2.7 951,621 + BBR_RS14155 0.46 +2.3 951,621 + BBR_RS14155 0.46 -1.6 951,659 - BBR_RS14155 0.49 +2.4 951,678 - BBR_RS14155 0.50 -0.4 951,697 + BBR_RS14155 0.51 -0.2 951,737 + BBR_RS14155 0.54 +1.0 951,751 + BBR_RS14155 0.55 -0.3 951,755 - BBR_RS14155 0.55 +1.4 951,796 - BBR_RS14155 0.58 +0.6
Or see this region's nucleotide sequence