Experiment: reconstituted MRS with Difucosyllactose 7.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14125 and BBR_RS14130 are separated by 90 nucleotides BBR_RS14130 and BBR_RS14135 overlap by 4 nucleotides BBR_RS14135 and BBR_RS14140 are separated by 58 nucleotides
BBR_RS14125: BBR_RS14125 - phosphopyruvate hydratase, at 946,410 to 947,708
_RS14125
BBR_RS14130: BBR_RS14130 - septum formation initiator family protein, at 947,799 to 948,383
_RS14130
BBR_RS14135: BBR_RS14135 - DUF501 domain-containing protein, at 948,380 to 948,946
_RS14135
BBR_RS14140: BBR_RS14140 - Ppx/GppA family phosphatase, at 949,005 to 950,006
_RS14140
Position (kb) 948
949 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7 at 947.718 kb on + strand at 948.219 kb on + strand, within BBR_RS14130 at 948.321 kb on + strand, within BBR_RS14130 at 948.353 kb on + strand at 948.353 kb on + strand at 948.358 kb on + strand at 948.366 kb on - strand at 948.378 kb on - strand at 948.403 kb on + strand at 948.403 kb on + strand at 948.411 kb on - strand at 948.895 kb on + strand at 948.907 kb on + strand at 948.912 kb on + strand at 948.935 kb on + strand at 948.943 kb on - strand at 948.961 kb on - strand at 948.979 kb on - strand at 949.002 kb on - strand at 949.470 kb on + strand, within BBR_RS14140 at 949.581 kb on + strand, within BBR_RS14140 at 949.630 kb on - strand, within BBR_RS14140 at 949.667 kb on + strand, within BBR_RS14140 at 949.777 kb on + strand, within BBR_RS14140 at 949.931 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM remove 947,718 + +2.6 948,219 + BBR_RS14130 0.72 -0.4 948,321 + BBR_RS14130 0.89 +0.2 948,353 + +0.4 948,353 + -0.5 948,358 + +0.4 948,366 - +3.7 948,378 - +0.6 948,403 + +2.5 948,403 + -2.1 948,411 - +0.9 948,895 + -0.6 948,907 + +5.4 948,912 + +0.8 948,935 + +3.5 948,943 - +1.4 948,961 - +1.3 948,979 - +0.0 949,002 - +2.1 949,470 + BBR_RS14140 0.46 +0.8 949,581 + BBR_RS14140 0.57 +2.1 949,630 - BBR_RS14140 0.62 -0.5 949,667 + BBR_RS14140 0.66 +7.7 949,777 + BBR_RS14140 0.77 -0.6 949,931 - +0.9
Or see this region's nucleotide sequence