Experiment: reconstituted MRS with Difucosyllactose 7.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13935 and BBR_RS13940 are separated by 158 nucleotides BBR_RS13940 and BBR_RS13945 are separated by 168 nucleotides BBR_RS13945 and BBR_RS13950 are separated by 1 nucleotides BBR_RS13950 and BBR_RS20810 are separated by 108 nucleotides BBR_RS20810 and BBR_RS13955 are separated by 9 nucleotides
BBR_RS13935: BBR_RS13935 - hypothetical protein, at 906,259 to 906,630
_RS13935
BBR_RS13940: BBR_RS13940 - integrase, at 906,789 to 907,040
_RS13940
BBR_RS13945: BBR_RS13945 - Txe/YoeB family addiction module toxin, at 907,209 to 907,484
_RS13945
BBR_RS13950: BBR_RS13950 - type II toxin-antitoxin system antitoxin, RelB/DinJ family, at 907,486 to 907,740
_RS13950
BBR_RS20810: BBR_RS20810 - pirin, at 907,849 to 907,959
_RS20810
BBR_RS13955: BBR_RS13955 - argininosuccinate lyase, at 907,969 to 909,441
_RS13955
Position (kb) 907
908 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 906.286 kb on + strand at 906.345 kb on + strand, within BBR_RS13935 at 906.372 kb on + strand, within BBR_RS13935 at 906.377 kb on + strand, within BBR_RS13935 at 906.385 kb on - strand, within BBR_RS13935 at 906.428 kb on - strand, within BBR_RS13935 at 906.453 kb on + strand, within BBR_RS13935 at 906.468 kb on - strand, within BBR_RS13935 at 906.489 kb on + strand, within BBR_RS13935 at 906.494 kb on + strand, within BBR_RS13935 at 906.723 kb on - strand at 906.724 kb on + strand at 906.724 kb on + strand at 906.806 kb on + strand at 906.835 kb on - strand, within BBR_RS13940 at 906.892 kb on + strand, within BBR_RS13940 at 906.895 kb on - strand, within BBR_RS13940 at 906.935 kb on - strand, within BBR_RS13940 at 906.935 kb on - strand, within BBR_RS13940 at 906.989 kb on + strand, within BBR_RS13940 at 907.005 kb on + strand, within BBR_RS13940 at 907.072 kb on - strand at 907.078 kb on - strand at 907.078 kb on - strand at 907.153 kb on - strand at 907.155 kb on - strand at 907.158 kb on - strand at 907.158 kb on - strand at 907.174 kb on - strand at 907.183 kb on + strand at 907.184 kb on - strand at 907.207 kb on + strand at 907.207 kb on + strand at 907.207 kb on + strand at 907.207 kb on + strand at 907.214 kb on + strand at 907.215 kb on - strand at 907.215 kb on - strand at 907.234 kb on - strand at 907.307 kb on + strand, within BBR_RS13945 at 907.350 kb on - strand, within BBR_RS13945 at 907.360 kb on - strand, within BBR_RS13945 at 907.434 kb on + strand, within BBR_RS13945 at 907.479 kb on + strand at 907.904 kb on + strand, within BBR_RS20810 at 907.920 kb on + strand, within BBR_RS20810 at 907.920 kb on + strand, within BBR_RS20810 at 907.953 kb on + strand at 907.953 kb on + strand at 907.961 kb on - strand at 907.977 kb on + strand at 907.995 kb on - strand at 908.037 kb on + strand at 908.046 kb on + strand at 908.046 kb on + strand at 908.054 kb on - strand at 908.054 kb on - strand at 908.054 kb on - strand at 908.109 kb on + strand at 908.116 kb on + strand at 908.116 kb on + strand at 908.141 kb on + strand, within BBR_RS13955 at 908.144 kb on - strand, within BBR_RS13955 at 908.152 kb on + strand, within BBR_RS13955 at 908.165 kb on - strand, within BBR_RS13955 at 908.182 kb on + strand, within BBR_RS13955 at 908.182 kb on + strand, within BBR_RS13955 at 908.182 kb on + strand, within BBR_RS13955 at 908.190 kb on - strand, within BBR_RS13955 at 908.207 kb on + strand, within BBR_RS13955 at 908.247 kb on + strand, within BBR_RS13955 at 908.281 kb on + strand, within BBR_RS13955 at 908.401 kb on + strand, within BBR_RS13955 at 908.402 kb on + strand, within BBR_RS13955 at 908.457 kb on + strand, within BBR_RS13955
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM remove 906,286 + -1.2 906,345 + BBR_RS13935 0.23 +3.1 906,372 + BBR_RS13935 0.30 +3.8 906,377 + BBR_RS13935 0.32 -1.5 906,385 - BBR_RS13935 0.34 +1.7 906,428 - BBR_RS13935 0.45 -0.4 906,453 + BBR_RS13935 0.52 -0.1 906,468 - BBR_RS13935 0.56 +3.1 906,489 + BBR_RS13935 0.62 +2.1 906,494 + BBR_RS13935 0.63 +3.0 906,723 - +3.3 906,724 + +2.1 906,724 + -1.4 906,806 + +1.8 906,835 - BBR_RS13940 0.18 +2.3 906,892 + BBR_RS13940 0.41 -1.6 906,895 - BBR_RS13940 0.42 +1.6 906,935 - BBR_RS13940 0.58 +1.0 906,935 - BBR_RS13940 0.58 +2.6 906,989 + BBR_RS13940 0.79 +0.2 907,005 + BBR_RS13940 0.86 +2.3 907,072 - +4.5 907,078 - +2.3 907,078 - -1.3 907,153 - +0.5 907,155 - +3.4 907,158 - -0.7 907,158 - +1.9 907,174 - -0.4 907,183 + +1.8 907,184 - -3.0 907,207 + +3.1 907,207 + +1.6 907,207 + -1.0 907,207 + +2.5 907,214 + +2.7 907,215 - +1.8 907,215 - +1.3 907,234 - -1.0 907,307 + BBR_RS13945 0.36 +0.6 907,350 - BBR_RS13945 0.51 +0.7 907,360 - BBR_RS13945 0.55 +1.0 907,434 + BBR_RS13945 0.82 -1.5 907,479 + +1.8 907,904 + BBR_RS20810 0.50 -0.4 907,920 + BBR_RS20810 0.64 -1.8 907,920 + BBR_RS20810 0.64 +3.6 907,953 + +4.2 907,953 + +2.0 907,961 - +0.6 907,977 + +0.2 907,995 - +0.1 908,037 + +2.5 908,046 + -1.0 908,046 + +1.3 908,054 - +0.8 908,054 - +1.3 908,054 - -1.3 908,109 + +0.0 908,116 + -1.5 908,116 + -0.4 908,141 + BBR_RS13955 0.12 -0.1 908,144 - BBR_RS13955 0.12 -0.2 908,152 + BBR_RS13955 0.12 -0.9 908,165 - BBR_RS13955 0.13 -1.4 908,182 + BBR_RS13955 0.14 -0.2 908,182 + BBR_RS13955 0.14 -1.0 908,182 + BBR_RS13955 0.14 +1.2 908,190 - BBR_RS13955 0.15 -1.6 908,207 + BBR_RS13955 0.16 +2.4 908,247 + BBR_RS13955 0.19 +1.5 908,281 + BBR_RS13955 0.21 +3.8 908,401 + BBR_RS13955 0.29 -0.5 908,402 + BBR_RS13955 0.29 -0.5 908,457 + BBR_RS13955 0.33 +0.4
Or see this region's nucleotide sequence