Experiment: reconstituted MRS with Difucosyllactose 7.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13635 and BBR_RS13640 overlap by 1 nucleotides BBR_RS13640 and BBR_RS13645 are separated by 36 nucleotides BBR_RS13645 and BBR_RS13650 are separated by 33 nucleotides
BBR_RS13635: BBR_RS13635 - carbamoyl-phosphate synthase large subunit, at 831,142 to 834,525
_RS13635
BBR_RS13640: BBR_RS13640 - orotidine-5'-phosphate decarboxylase, at 834,525 to 835,445
_RS13640
BBR_RS13645: BBR_RS13645 - guanylate kinase, at 835,482 to 836,072
_RS13645
BBR_RS13650: BBR_RS13650 - 3'-5' exonuclease, at 836,106 to 836,813
_RS13650
Position (kb) 834
835
836 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 833.552 kb on + strand, within BBR_RS13635 at 833.597 kb on - strand, within BBR_RS13635 at 833.610 kb on + strand, within BBR_RS13635 at 833.649 kb on + strand, within BBR_RS13635 at 833.656 kb on + strand, within BBR_RS13635 at 833.717 kb on + strand, within BBR_RS13635 at 833.717 kb on + strand, within BBR_RS13635 at 833.832 kb on + strand, within BBR_RS13635 at 833.833 kb on + strand, within BBR_RS13635 at 833.892 kb on + strand, within BBR_RS13635 at 833.937 kb on + strand, within BBR_RS13635 at 833.942 kb on + strand, within BBR_RS13635 at 833.974 kb on + strand, within BBR_RS13635 at 833.975 kb on - strand, within BBR_RS13635 at 834.110 kb on + strand, within BBR_RS13635 at 834.125 kb on + strand, within BBR_RS13635 at 834.219 kb on - strand at 834.450 kb on + strand at 834.519 kb on + strand at 834.657 kb on + strand, within BBR_RS13640 at 834.679 kb on + strand, within BBR_RS13640 at 834.679 kb on + strand, within BBR_RS13640 at 834.679 kb on + strand, within BBR_RS13640 at 834.786 kb on + strand, within BBR_RS13640 at 834.800 kb on + strand, within BBR_RS13640 at 834.800 kb on + strand, within BBR_RS13640 at 834.800 kb on + strand, within BBR_RS13640 at 834.813 kb on + strand, within BBR_RS13640 at 834.815 kb on + strand, within BBR_RS13640 at 834.822 kb on + strand, within BBR_RS13640 at 834.834 kb on + strand, within BBR_RS13640 at 834.893 kb on + strand, within BBR_RS13640 at 834.893 kb on + strand, within BBR_RS13640 at 835.021 kb on + strand, within BBR_RS13640 at 835.036 kb on + strand, within BBR_RS13640 at 835.121 kb on + strand, within BBR_RS13640 at 835.141 kb on + strand, within BBR_RS13640 at 835.156 kb on + strand, within BBR_RS13640 at 835.174 kb on + strand, within BBR_RS13640 at 835.200 kb on + strand, within BBR_RS13640 at 835.281 kb on + strand, within BBR_RS13640 at 835.448 kb on - strand at 835.493 kb on + strand at 836.057 kb on + strand at 836.109 kb on + strand at 836.117 kb on - strand at 836.130 kb on + strand at 836.148 kb on + strand at 836.148 kb on + strand at 836.156 kb on - strand at 836.156 kb on - strand at 836.165 kb on + strand at 836.165 kb on + strand at 836.243 kb on - strand, within BBR_RS13650 at 836.270 kb on - strand, within BBR_RS13650 at 836.272 kb on - strand, within BBR_RS13650 at 836.276 kb on + strand, within BBR_RS13650 at 836.324 kb on + strand, within BBR_RS13650 at 836.324 kb on + strand, within BBR_RS13650 at 836.339 kb on - strand, within BBR_RS13650 at 836.409 kb on - strand, within BBR_RS13650
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM remove 833,552 + BBR_RS13635 0.71 +1.1 833,597 - BBR_RS13635 0.73 +0.9 833,610 + BBR_RS13635 0.73 +1.8 833,649 + BBR_RS13635 0.74 +0.7 833,656 + BBR_RS13635 0.74 +0.9 833,717 + BBR_RS13635 0.76 +2.6 833,717 + BBR_RS13635 0.76 +2.0 833,832 + BBR_RS13635 0.79 +1.8 833,833 + BBR_RS13635 0.80 +1.6 833,892 + BBR_RS13635 0.81 +2.6 833,937 + BBR_RS13635 0.83 +0.3 833,942 + BBR_RS13635 0.83 +1.3 833,974 + BBR_RS13635 0.84 +2.6 833,975 - BBR_RS13635 0.84 +1.8 834,110 + BBR_RS13635 0.88 +1.9 834,125 + BBR_RS13635 0.88 +0.2 834,219 - +3.9 834,450 + +2.6 834,519 + -1.0 834,657 + BBR_RS13640 0.14 -0.3 834,679 + BBR_RS13640 0.17 -0.4 834,679 + BBR_RS13640 0.17 -1.0 834,679 + BBR_RS13640 0.17 -1.1 834,786 + BBR_RS13640 0.28 +1.6 834,800 + BBR_RS13640 0.30 -0.4 834,800 + BBR_RS13640 0.30 -0.7 834,800 + BBR_RS13640 0.30 -1.1 834,813 + BBR_RS13640 0.31 -0.9 834,815 + BBR_RS13640 0.31 +1.2 834,822 + BBR_RS13640 0.32 +0.9 834,834 + BBR_RS13640 0.34 -1.1 834,893 + BBR_RS13640 0.40 -0.5 834,893 + BBR_RS13640 0.40 +0.5 835,021 + BBR_RS13640 0.54 +0.1 835,036 + BBR_RS13640 0.55 -0.5 835,121 + BBR_RS13640 0.65 +0.3 835,141 + BBR_RS13640 0.67 -0.4 835,156 + BBR_RS13640 0.69 -0.4 835,174 + BBR_RS13640 0.70 -1.8 835,200 + BBR_RS13640 0.73 -1.2 835,281 + BBR_RS13640 0.82 -0.4 835,448 - +2.9 835,493 + +3.4 836,057 + +0.4 836,109 + -0.5 836,117 - +2.7 836,130 + +0.1 836,148 + +2.8 836,148 + +2.5 836,156 - -1.8 836,156 - +1.2 836,165 + +2.3 836,165 + +1.4 836,243 - BBR_RS13650 0.19 -1.1 836,270 - BBR_RS13650 0.23 +3.7 836,272 - BBR_RS13650 0.23 +1.3 836,276 + BBR_RS13650 0.24 -0.2 836,324 + BBR_RS13650 0.31 -1.2 836,324 + BBR_RS13650 0.31 -0.7 836,339 - BBR_RS13650 0.33 +2.9 836,409 - BBR_RS13650 0.43 -0.6
Or see this region's nucleotide sequence