Experiment: reconstituted MRS with Difucosyllactose 7.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13545 and BBR_RS13550 are separated by 108 nucleotides BBR_RS13550 and BBR_RS20780 overlap by 70 nucleotides BBR_RS20780 and BBR_RS13555 overlap by 2 nucleotides BBR_RS13555 and BBR_RS13560 are separated by 175 nucleotides
BBR_RS13545: BBR_RS13545 - DUF4143 domain-containing protein, at 810,002 to 811,363
_RS13545
BBR_RS13550: BBR_RS13550 - DUF47 domain-containing protein, at 811,472 to 812,089
_RS13550
BBR_RS20780: BBR_RS20780 - hypothetical protein, at 812,020 to 812,304
_RS20780
BBR_RS13555: BBR_RS13555 - excinuclease ABC subunit A, at 812,303 to 814,807
_RS13555
BBR_RS13560: BBR_RS13560 - MATE family efflux transporter, at 814,983 to 816,341
_RS13560
Position (kb) 812
813
814
815 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 811.342 kb on + strand at 811.342 kb on + strand at 811.352 kb on - strand at 811.469 kb on + strand at 811.479 kb on - strand at 811.515 kb on - strand at 811.519 kb on + strand at 811.519 kb on + strand at 811.519 kb on + strand at 811.525 kb on - strand at 811.527 kb on - strand at 811.527 kb on - strand at 811.533 kb on - strand at 811.596 kb on + strand, within BBR_RS13550 at 811.613 kb on + strand, within BBR_RS13550 at 811.640 kb on + strand, within BBR_RS13550 at 811.675 kb on - strand, within BBR_RS13550 at 811.678 kb on + strand, within BBR_RS13550 at 811.678 kb on + strand, within BBR_RS13550 at 811.720 kb on + strand, within BBR_RS13550 at 811.736 kb on + strand, within BBR_RS13550 at 811.746 kb on + strand, within BBR_RS13550 at 811.759 kb on - strand, within BBR_RS13550 at 811.787 kb on - strand, within BBR_RS13550 at 811.811 kb on + strand, within BBR_RS13550 at 811.844 kb on + strand, within BBR_RS13550 at 811.859 kb on + strand, within BBR_RS13550 at 811.859 kb on + strand, within BBR_RS13550 at 811.859 kb on + strand, within BBR_RS13550 at 811.860 kb on - strand, within BBR_RS13550 at 811.865 kb on - strand, within BBR_RS13550 at 811.867 kb on - strand, within BBR_RS13550 at 811.880 kb on - strand, within BBR_RS13550 at 811.886 kb on - strand, within BBR_RS13550 at 811.965 kb on + strand, within BBR_RS13550 at 811.975 kb on + strand, within BBR_RS13550 at 811.981 kb on - strand, within BBR_RS13550 at 811.997 kb on + strand, within BBR_RS13550 at 812.002 kb on + strand, within BBR_RS13550 at 812.016 kb on + strand, within BBR_RS13550 at 812.028 kb on + strand at 812.177 kb on + strand, within BBR_RS20780 at 812.185 kb on + strand, within BBR_RS20780 at 812.226 kb on - strand, within BBR_RS20780 at 812.272 kb on + strand, within BBR_RS20780 at 812.275 kb on + strand, within BBR_RS20780 at 812.281 kb on - strand at 812.289 kb on + strand at 812.294 kb on + strand at 812.336 kb on - strand at 812.359 kb on + strand at 812.408 kb on + strand at 812.491 kb on + strand at 812.496 kb on + strand at 812.524 kb on - strand at 812.534 kb on - strand at 812.534 kb on - strand at 812.541 kb on + strand at 812.546 kb on + strand at 812.546 kb on + strand at 812.546 kb on + strand at 812.554 kb on - strand, within BBR_RS13555 at 812.573 kb on - strand, within BBR_RS13555 at 812.611 kb on - strand, within BBR_RS13555 at 812.632 kb on + strand, within BBR_RS13555 at 812.632 kb on + strand, within BBR_RS13555 at 812.632 kb on + strand, within BBR_RS13555 at 812.632 kb on + strand, within BBR_RS13555 at 812.640 kb on - strand, within BBR_RS13555 at 812.640 kb on - strand, within BBR_RS13555 at 812.640 kb on - strand, within BBR_RS13555 at 812.640 kb on - strand, within BBR_RS13555 at 812.640 kb on - strand, within BBR_RS13555 at 812.640 kb on - strand, within BBR_RS13555 at 812.655 kb on - strand, within BBR_RS13555 at 812.667 kb on + strand, within BBR_RS13555 at 812.731 kb on + strand, within BBR_RS13555 at 812.740 kb on - strand, within BBR_RS13555 at 812.740 kb on - strand, within BBR_RS13555 at 812.742 kb on + strand, within BBR_RS13555 at 812.745 kb on - strand, within BBR_RS13555 at 812.818 kb on - strand, within BBR_RS13555 at 812.839 kb on + strand, within BBR_RS13555 at 812.865 kb on + strand, within BBR_RS13555 at 812.930 kb on - strand, within BBR_RS13555 at 812.939 kb on - strand, within BBR_RS13555 at 812.944 kb on - strand, within BBR_RS13555 at 812.991 kb on + strand, within BBR_RS13555 at 812.991 kb on + strand, within BBR_RS13555 at 812.999 kb on - strand, within BBR_RS13555 at 813.094 kb on + strand, within BBR_RS13555 at 813.094 kb on + strand, within BBR_RS13555 at 813.094 kb on + strand, within BBR_RS13555 at 813.094 kb on + strand, within BBR_RS13555 at 813.101 kb on + strand, within BBR_RS13555 at 813.106 kb on + strand, within BBR_RS13555 at 813.109 kb on - strand, within BBR_RS13555 at 813.113 kb on + strand, within BBR_RS13555 at 813.118 kb on - strand, within BBR_RS13555 at 813.135 kb on - strand, within BBR_RS13555 at 813.149 kb on + strand, within BBR_RS13555 at 813.157 kb on - strand, within BBR_RS13555 at 813.157 kb on - strand, within BBR_RS13555 at 813.157 kb on - strand, within BBR_RS13555 at 813.231 kb on + strand, within BBR_RS13555 at 813.239 kb on - strand, within BBR_RS13555 at 813.243 kb on - strand, within BBR_RS13555 at 813.246 kb on + strand, within BBR_RS13555 at 813.246 kb on + strand, within BBR_RS13555 at 813.265 kb on - strand, within BBR_RS13555 at 813.277 kb on - strand, within BBR_RS13555 at 813.375 kb on - strand, within BBR_RS13555 at 813.471 kb on + strand, within BBR_RS13555 at 813.471 kb on + strand, within BBR_RS13555 at 813.471 kb on + strand, within BBR_RS13555 at 813.479 kb on - strand, within BBR_RS13555 at 813.501 kb on + strand, within BBR_RS13555 at 813.533 kb on - strand, within BBR_RS13555 at 813.538 kb on + strand, within BBR_RS13555 at 813.546 kb on - strand, within BBR_RS13555 at 813.567 kb on - strand, within BBR_RS13555 at 813.589 kb on + strand, within BBR_RS13555 at 813.611 kb on + strand, within BBR_RS13555 at 813.611 kb on + strand, within BBR_RS13555 at 813.613 kb on - strand, within BBR_RS13555 at 813.700 kb on + strand, within BBR_RS13555 at 813.708 kb on - strand, within BBR_RS13555 at 813.733 kb on - strand, within BBR_RS13555 at 813.803 kb on + strand, within BBR_RS13555 at 813.932 kb on + strand, within BBR_RS13555 at 813.962 kb on + strand, within BBR_RS13555 at 813.975 kb on - strand, within BBR_RS13555 at 813.989 kb on + strand, within BBR_RS13555 at 814.011 kb on - strand, within BBR_RS13555 at 814.050 kb on - strand, within BBR_RS13555 at 814.076 kb on - strand, within BBR_RS13555 at 814.087 kb on + strand, within BBR_RS13555 at 814.094 kb on + strand, within BBR_RS13555 at 814.108 kb on + strand, within BBR_RS13555 at 814.111 kb on - strand, within BBR_RS13555 at 814.143 kb on - strand, within BBR_RS13555 at 814.176 kb on + strand, within BBR_RS13555 at 814.176 kb on + strand, within BBR_RS13555 at 814.184 kb on - strand, within BBR_RS13555 at 814.184 kb on - strand, within BBR_RS13555 at 814.185 kb on - strand, within BBR_RS13555 at 814.189 kb on + strand, within BBR_RS13555 at 814.217 kb on - strand, within BBR_RS13555 at 814.232 kb on + strand, within BBR_RS13555 at 814.270 kb on + strand, within BBR_RS13555 at 814.318 kb on + strand, within BBR_RS13555 at 814.326 kb on - strand, within BBR_RS13555 at 814.351 kb on + strand, within BBR_RS13555 at 814.355 kb on - strand, within BBR_RS13555 at 814.409 kb on - strand, within BBR_RS13555 at 814.421 kb on + strand, within BBR_RS13555 at 814.421 kb on + strand, within BBR_RS13555 at 814.429 kb on - strand, within BBR_RS13555 at 814.484 kb on + strand, within BBR_RS13555 at 814.507 kb on + strand, within BBR_RS13555 at 814.578 kb on + strand at 814.578 kb on + strand at 814.578 kb on + strand at 814.578 kb on + strand at 814.578 kb on + strand at 814.614 kb on + strand at 814.623 kb on + strand at 814.628 kb on - strand at 814.631 kb on - strand at 814.636 kb on - strand at 814.654 kb on - strand at 814.667 kb on - strand at 814.696 kb on - strand at 814.705 kb on + strand at 814.760 kb on - strand at 814.805 kb on - strand at 814.809 kb on + strand at 814.810 kb on - strand at 814.825 kb on - strand at 814.857 kb on - strand at 814.936 kb on + strand at 814.955 kb on + strand at 814.955 kb on + strand at 814.979 kb on - strand at 814.991 kb on + strand at 815.050 kb on + strand at 815.127 kb on + strand, within BBR_RS13560 at 815.131 kb on - strand, within BBR_RS13560 at 815.147 kb on + strand, within BBR_RS13560 at 815.147 kb on + strand, within BBR_RS13560 at 815.147 kb on + strand, within BBR_RS13560 at 815.162 kb on + strand, within BBR_RS13560 at 815.162 kb on + strand, within BBR_RS13560 at 815.165 kb on - strand, within BBR_RS13560 at 815.165 kb on - strand, within BBR_RS13560 at 815.170 kb on - strand, within BBR_RS13560 at 815.171 kb on + strand, within BBR_RS13560 at 815.259 kb on - strand, within BBR_RS13560 at 815.284 kb on + strand, within BBR_RS13560 at 815.299 kb on - strand, within BBR_RS13560 at 815.392 kb on - strand, within BBR_RS13560 at 815.430 kb on - strand, within BBR_RS13560 at 815.521 kb on + strand, within BBR_RS13560 at 815.548 kb on - strand, within BBR_RS13560 at 815.614 kb on - strand, within BBR_RS13560 at 815.724 kb on - strand, within BBR_RS13560 at 815.734 kb on - strand, within BBR_RS13560 at 815.792 kb on + strand, within BBR_RS13560
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM remove 811,342 + +2.4 811,342 + -0.4 811,352 - -0.6 811,469 + +2.6 811,479 - -1.1 811,515 - +2.3 811,519 + +2.6 811,519 + +1.5 811,519 + -0.7 811,525 - +1.8 811,527 - +0.4 811,527 - +2.9 811,533 - +0.3 811,596 + BBR_RS13550 0.20 -0.9 811,613 + BBR_RS13550 0.23 +0.0 811,640 + BBR_RS13550 0.27 +0.7 811,675 - BBR_RS13550 0.33 -1.2 811,678 + BBR_RS13550 0.33 +0.3 811,678 + BBR_RS13550 0.33 -2.6 811,720 + BBR_RS13550 0.40 -0.8 811,736 + BBR_RS13550 0.43 -0.7 811,746 + BBR_RS13550 0.44 +2.4 811,759 - BBR_RS13550 0.46 -1.2 811,787 - BBR_RS13550 0.51 -0.1 811,811 + BBR_RS13550 0.55 +0.8 811,844 + BBR_RS13550 0.60 +0.3 811,859 + BBR_RS13550 0.63 +0.7 811,859 + BBR_RS13550 0.63 -1.0 811,859 + BBR_RS13550 0.63 +0.1 811,860 - BBR_RS13550 0.63 +2.4 811,865 - BBR_RS13550 0.64 -0.9 811,867 - BBR_RS13550 0.64 -0.6 811,880 - BBR_RS13550 0.66 +1.4 811,886 - BBR_RS13550 0.67 +0.4 811,965 + BBR_RS13550 0.80 -2.0 811,975 + BBR_RS13550 0.81 -1.6 811,981 - BBR_RS13550 0.82 +1.3 811,997 + BBR_RS13550 0.85 -0.6 812,002 + BBR_RS13550 0.86 +2.0 812,016 + BBR_RS13550 0.88 -0.9 812,028 + -1.5 812,177 + BBR_RS20780 0.55 +0.3 812,185 + BBR_RS20780 0.58 -1.1 812,226 - BBR_RS20780 0.72 -2.8 812,272 + BBR_RS20780 0.88 -0.4 812,275 + BBR_RS20780 0.89 +2.7 812,281 - +3.0 812,289 + -0.0 812,294 + +4.1 812,336 - +2.6 812,359 + -1.1 812,408 + +4.0 812,491 + +1.1 812,496 + -1.7 812,524 - -1.4 812,534 - +1.5 812,534 - +2.1 812,541 + +2.2 812,546 + -0.2 812,546 + -1.8 812,546 + +0.1 812,554 - BBR_RS13555 0.10 +2.5 812,573 - BBR_RS13555 0.11 +1.5 812,611 - BBR_RS13555 0.12 +1.2 812,632 + BBR_RS13555 0.13 -1.1 812,632 + BBR_RS13555 0.13 -0.7 812,632 + BBR_RS13555 0.13 -1.6 812,632 + BBR_RS13555 0.13 +2.7 812,640 - BBR_RS13555 0.13 -1.2 812,640 - BBR_RS13555 0.13 -0.8 812,640 - BBR_RS13555 0.13 -2.4 812,640 - BBR_RS13555 0.13 -0.9 812,640 - BBR_RS13555 0.13 +1.3 812,640 - BBR_RS13555 0.13 -0.5 812,655 - BBR_RS13555 0.14 -1.2 812,667 + BBR_RS13555 0.15 -1.6 812,731 + BBR_RS13555 0.17 +3.4 812,740 - BBR_RS13555 0.17 -1.7 812,740 - BBR_RS13555 0.17 -2.0 812,742 + BBR_RS13555 0.18 +0.6 812,745 - BBR_RS13555 0.18 -1.9 812,818 - BBR_RS13555 0.21 +2.6 812,839 + BBR_RS13555 0.21 -0.4 812,865 + BBR_RS13555 0.22 +3.2 812,930 - BBR_RS13555 0.25 -0.6 812,939 - BBR_RS13555 0.25 -1.3 812,944 - BBR_RS13555 0.26 -1.0 812,991 + BBR_RS13555 0.27 +1.2 812,991 + BBR_RS13555 0.27 -0.5 812,999 - BBR_RS13555 0.28 +2.1 813,094 + BBR_RS13555 0.32 +0.9 813,094 + BBR_RS13555 0.32 +3.2 813,094 + BBR_RS13555 0.32 +1.9 813,094 + BBR_RS13555 0.32 +0.1 813,101 + BBR_RS13555 0.32 +3.5 813,106 + BBR_RS13555 0.32 -1.3 813,109 - BBR_RS13555 0.32 -0.8 813,113 + BBR_RS13555 0.32 -2.0 813,118 - BBR_RS13555 0.33 -0.9 813,135 - BBR_RS13555 0.33 +3.2 813,149 + BBR_RS13555 0.34 +2.7 813,157 - BBR_RS13555 0.34 +1.0 813,157 - BBR_RS13555 0.34 +2.1 813,157 - BBR_RS13555 0.34 -0.3 813,231 + BBR_RS13555 0.37 -1.4 813,239 - BBR_RS13555 0.37 -0.5 813,243 - BBR_RS13555 0.38 -0.4 813,246 + BBR_RS13555 0.38 -1.6 813,246 + BBR_RS13555 0.38 -1.7 813,265 - BBR_RS13555 0.38 -1.2 813,277 - BBR_RS13555 0.39 -1.1 813,375 - BBR_RS13555 0.43 +3.2 813,471 + BBR_RS13555 0.47 -0.4 813,471 + BBR_RS13555 0.47 -0.2 813,471 + BBR_RS13555 0.47 +1.1 813,479 - BBR_RS13555 0.47 -0.4 813,501 + BBR_RS13555 0.48 -0.9 813,533 - BBR_RS13555 0.49 -0.9 813,538 + BBR_RS13555 0.49 -1.8 813,546 - BBR_RS13555 0.50 +0.5 813,567 - BBR_RS13555 0.50 -1.4 813,589 + BBR_RS13555 0.51 -1.4 813,611 + BBR_RS13555 0.52 +0.1 813,611 + BBR_RS13555 0.52 -1.9 813,613 - BBR_RS13555 0.52 -0.9 813,700 + BBR_RS13555 0.56 +2.9 813,708 - BBR_RS13555 0.56 -0.5 813,733 - BBR_RS13555 0.57 -0.7 813,803 + BBR_RS13555 0.60 -0.8 813,932 + BBR_RS13555 0.65 -0.5 813,962 + BBR_RS13555 0.66 +2.6 813,975 - BBR_RS13555 0.67 -0.1 813,989 + BBR_RS13555 0.67 -0.7 814,011 - BBR_RS13555 0.68 -2.5 814,050 - BBR_RS13555 0.70 -0.6 814,076 - BBR_RS13555 0.71 +0.5 814,087 + BBR_RS13555 0.71 -0.6 814,094 + BBR_RS13555 0.71 -1.2 814,108 + BBR_RS13555 0.72 +2.0 814,111 - BBR_RS13555 0.72 -1.1 814,143 - BBR_RS13555 0.73 -1.5 814,176 + BBR_RS13555 0.75 +1.9 814,176 + BBR_RS13555 0.75 +1.4 814,184 - BBR_RS13555 0.75 +0.9 814,184 - BBR_RS13555 0.75 -0.4 814,185 - BBR_RS13555 0.75 +0.2 814,189 + BBR_RS13555 0.75 +4.2 814,217 - BBR_RS13555 0.76 -1.0 814,232 + BBR_RS13555 0.77 +0.5 814,270 + BBR_RS13555 0.79 -0.2 814,318 + BBR_RS13555 0.80 +0.1 814,326 - BBR_RS13555 0.81 +0.8 814,351 + BBR_RS13555 0.82 +0.2 814,355 - BBR_RS13555 0.82 -0.3 814,409 - BBR_RS13555 0.84 +1.2 814,421 + BBR_RS13555 0.85 -0.4 814,421 + BBR_RS13555 0.85 -0.7 814,429 - BBR_RS13555 0.85 -0.7 814,484 + BBR_RS13555 0.87 +3.3 814,507 + BBR_RS13555 0.88 -0.7 814,578 + +1.0 814,578 + -0.2 814,578 + +0.8 814,578 + +0.7 814,578 + +2.5 814,614 + +0.3 814,623 + -1.2 814,628 - -2.1 814,631 - +4.4 814,636 - +2.3 814,654 - +2.8 814,667 - +2.0 814,696 - +1.6 814,705 + -0.9 814,760 - -0.2 814,805 - -0.1 814,809 + +1.3 814,810 - -0.7 814,825 - -0.2 814,857 - -0.7 814,936 + -1.2 814,955 + +2.3 814,955 + +1.4 814,979 - +0.6 814,991 + -0.1 815,050 + -0.0 815,127 + BBR_RS13560 0.11 -0.6 815,131 - BBR_RS13560 0.11 +2.2 815,147 + BBR_RS13560 0.12 +2.3 815,147 + BBR_RS13560 0.12 -2.2 815,147 + BBR_RS13560 0.12 +1.1 815,162 + BBR_RS13560 0.13 +1.6 815,162 + BBR_RS13560 0.13 -0.8 815,165 - BBR_RS13560 0.13 +3.1 815,165 - BBR_RS13560 0.13 -0.2 815,170 - BBR_RS13560 0.14 -1.3 815,171 + BBR_RS13560 0.14 +0.3 815,259 - BBR_RS13560 0.20 +3.0 815,284 + BBR_RS13560 0.22 -0.5 815,299 - BBR_RS13560 0.23 +0.2 815,392 - BBR_RS13560 0.30 -0.2 815,430 - BBR_RS13560 0.33 -0.0 815,521 + BBR_RS13560 0.40 +2.5 815,548 - BBR_RS13560 0.42 -1.5 815,614 - BBR_RS13560 0.46 -1.0 815,724 - BBR_RS13560 0.55 -0.2 815,734 - BBR_RS13560 0.55 -1.2 815,792 + BBR_RS13560 0.60 -1.0
Or see this region's nucleotide sequence