Experiment: reconstituted MRS with Difucosyllactose 7.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13455 and BBR_RS13460 are separated by 499 nucleotides BBR_RS13460 and BBR_RS13465 are separated by 116 nucleotides
BBR_RS13455: BBR_RS13455 - formate-dependent phosphoribosylglycinamide formyltransferase, at 791,539 to 792,840
_RS13455
BBR_RS13460: BBR_RS13460 - aminotransferase, at 793,340 to 794,590
_RS13460
BBR_RS13465: BBR_RS13465 - glycosyl transferase, at 794,707 to 795,867
_RS13465
Position (kb) 793
794
795 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 792.495 kb on + strand, within BBR_RS13455 at 792.534 kb on - strand, within BBR_RS13455 at 792.549 kb on + strand, within BBR_RS13455 at 792.562 kb on - strand, within BBR_RS13455 at 792.750 kb on - strand at 792.759 kb on + strand at 792.828 kb on - strand at 792.837 kb on - strand at 792.853 kb on - strand at 792.865 kb on + strand at 792.865 kb on + strand at 792.865 kb on + strand at 792.865 kb on + strand at 792.865 kb on + strand at 792.865 kb on + strand at 792.873 kb on - strand at 792.873 kb on - strand at 792.887 kb on + strand at 792.929 kb on - strand at 792.929 kb on - strand at 792.931 kb on - strand at 792.999 kb on + strand at 792.999 kb on + strand at 792.999 kb on + strand at 792.999 kb on + strand at 792.999 kb on + strand at 792.999 kb on + strand at 793.006 kb on - strand at 793.007 kb on - strand at 793.007 kb on - strand at 793.007 kb on - strand at 793.022 kb on - strand at 793.048 kb on + strand at 793.050 kb on + strand at 793.050 kb on + strand at 793.096 kb on + strand at 793.106 kb on + strand at 793.114 kb on - strand at 793.114 kb on - strand at 793.116 kb on + strand at 793.121 kb on + strand at 793.124 kb on - strand at 793.129 kb on + strand at 793.129 kb on + strand at 793.131 kb on + strand at 793.158 kb on + strand at 793.262 kb on + strand at 793.262 kb on + strand at 793.270 kb on - strand at 793.274 kb on + strand at 793.312 kb on + strand at 793.325 kb on - strand at 793.325 kb on - strand at 793.325 kb on - strand at 793.325 kb on - strand at 793.352 kb on + strand at 793.369 kb on + strand at 793.418 kb on - strand at 793.488 kb on + strand, within BBR_RS13460 at 793.597 kb on - strand, within BBR_RS13460 at 793.598 kb on + strand, within BBR_RS13460 at 793.599 kb on - strand, within BBR_RS13460 at 793.614 kb on + strand, within BBR_RS13460 at 793.616 kb on - strand, within BBR_RS13460 at 793.761 kb on - strand, within BBR_RS13460 at 793.821 kb on + strand, within BBR_RS13460 at 793.829 kb on - strand, within BBR_RS13460 at 793.835 kb on + strand, within BBR_RS13460 at 793.843 kb on - strand, within BBR_RS13460 at 793.843 kb on - strand, within BBR_RS13460 at 793.890 kb on - strand, within BBR_RS13460 at 793.899 kb on + strand, within BBR_RS13460 at 793.907 kb on - strand, within BBR_RS13460 at 793.907 kb on - strand, within BBR_RS13460 at 793.909 kb on + strand, within BBR_RS13460 at 793.909 kb on + strand, within BBR_RS13460 at 793.924 kb on + strand, within BBR_RS13460 at 793.924 kb on + strand, within BBR_RS13460 at 793.939 kb on - strand, within BBR_RS13460 at 793.947 kb on - strand, within BBR_RS13460 at 793.953 kb on + strand, within BBR_RS13460 at 793.971 kb on + strand, within BBR_RS13460 at 794.017 kb on - strand, within BBR_RS13460 at 794.061 kb on + strand, within BBR_RS13460 at 794.094 kb on - strand, within BBR_RS13460 at 794.117 kb on + strand, within BBR_RS13460 at 794.126 kb on + strand, within BBR_RS13460 at 794.126 kb on - strand, within BBR_RS13460 at 794.128 kb on + strand, within BBR_RS13460 at 794.128 kb on + strand, within BBR_RS13460 at 794.128 kb on + strand, within BBR_RS13460 at 794.128 kb on + strand, within BBR_RS13460 at 794.128 kb on + strand, within BBR_RS13460 at 794.128 kb on + strand, within BBR_RS13460 at 794.128 kb on + strand, within BBR_RS13460 at 794.128 kb on + strand, within BBR_RS13460 at 794.128 kb on + strand, within BBR_RS13460 at 794.128 kb on + strand, within BBR_RS13460 at 794.128 kb on + strand, within BBR_RS13460 at 794.128 kb on + strand, within BBR_RS13460 at 794.128 kb on + strand, within BBR_RS13460 at 794.128 kb on + strand, within BBR_RS13460 at 794.128 kb on + strand, within BBR_RS13460 at 794.128 kb on + strand, within BBR_RS13460 at 794.133 kb on + strand, within BBR_RS13460 at 794.135 kb on + strand, within BBR_RS13460 at 794.136 kb on - strand, within BBR_RS13460 at 794.136 kb on - strand, within BBR_RS13460 at 794.136 kb on - strand, within BBR_RS13460 at 794.136 kb on - strand, within BBR_RS13460 at 794.136 kb on - strand, within BBR_RS13460 at 794.136 kb on - strand, within BBR_RS13460 at 794.136 kb on - strand, within BBR_RS13460 at 794.136 kb on - strand, within BBR_RS13460 at 794.136 kb on - strand, within BBR_RS13460 at 794.136 kb on - strand, within BBR_RS13460 at 794.136 kb on - strand, within BBR_RS13460 at 794.136 kb on - strand, within BBR_RS13460 at 794.136 kb on - strand, within BBR_RS13460 at 794.136 kb on - strand, within BBR_RS13460 at 794.143 kb on - strand, within BBR_RS13460 at 794.197 kb on + strand, within BBR_RS13460 at 794.207 kb on + strand, within BBR_RS13460 at 794.212 kb on + strand, within BBR_RS13460 at 794.215 kb on - strand, within BBR_RS13460 at 794.234 kb on + strand, within BBR_RS13460 at 794.259 kb on - strand, within BBR_RS13460 at 794.271 kb on + strand, within BBR_RS13460 at 794.271 kb on + strand, within BBR_RS13460 at 794.307 kb on + strand, within BBR_RS13460 at 794.311 kb on - strand, within BBR_RS13460 at 794.336 kb on + strand, within BBR_RS13460 at 794.352 kb on + strand, within BBR_RS13460 at 794.352 kb on - strand, within BBR_RS13460 at 794.382 kb on + strand, within BBR_RS13460 at 794.383 kb on + strand, within BBR_RS13460 at 794.383 kb on + strand, within BBR_RS13460 at 794.388 kb on + strand, within BBR_RS13460 at 794.391 kb on - strand, within BBR_RS13460 at 794.391 kb on - strand, within BBR_RS13460 at 794.391 kb on - strand, within BBR_RS13460 at 794.408 kb on - strand, within BBR_RS13460 at 794.452 kb on + strand, within BBR_RS13460 at 794.498 kb on + strand at 794.498 kb on + strand at 794.571 kb on - strand at 794.584 kb on - strand at 794.584 kb on - strand at 794.679 kb on + strand at 794.687 kb on - strand at 794.819 kb on - strand at 794.824 kb on - strand, within BBR_RS13465 at 794.840 kb on + strand, within BBR_RS13465 at 794.860 kb on + strand, within BBR_RS13465 at 794.874 kb on + strand, within BBR_RS13465 at 794.929 kb on - strand, within BBR_RS13465 at 794.940 kb on - strand, within BBR_RS13465 at 794.972 kb on - strand, within BBR_RS13465 at 795.008 kb on - strand, within BBR_RS13465 at 795.107 kb on - strand, within BBR_RS13465 at 795.110 kb on - strand, within BBR_RS13465 at 795.253 kb on - strand, within BBR_RS13465 at 795.316 kb on - strand, within BBR_RS13465 at 795.316 kb on - strand, within BBR_RS13465 at 795.365 kb on - strand, within BBR_RS13465 at 795.377 kb on + strand, within BBR_RS13465 at 795.387 kb on + strand, within BBR_RS13465 at 795.387 kb on + strand, within BBR_RS13465 at 795.387 kb on + strand, within BBR_RS13465 at 795.387 kb on + strand, within BBR_RS13465 at 795.387 kb on + strand, within BBR_RS13465 at 795.395 kb on - strand, within BBR_RS13465 at 795.395 kb on - strand, within BBR_RS13465 at 795.395 kb on - strand, within BBR_RS13465 at 795.395 kb on - strand, within BBR_RS13465 at 795.395 kb on - strand, within BBR_RS13465 at 795.395 kb on - strand, within BBR_RS13465 at 795.395 kb on - strand, within BBR_RS13465 at 795.395 kb on - strand, within BBR_RS13465 at 795.395 kb on - strand, within BBR_RS13465 at 795.443 kb on + strand, within BBR_RS13465 at 795.510 kb on - strand, within BBR_RS13465 at 795.554 kb on - strand, within BBR_RS13465 at 795.554 kb on - strand, within BBR_RS13465 at 795.561 kb on + strand, within BBR_RS13465
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM remove 792,495 + BBR_RS13455 0.73 +0.9 792,534 - BBR_RS13455 0.76 +1.1 792,549 + BBR_RS13455 0.78 +1.6 792,562 - BBR_RS13455 0.79 +1.4 792,750 - +2.1 792,759 + +1.7 792,828 - +2.6 792,837 - -0.5 792,853 - +1.0 792,865 + -1.2 792,865 + -1.5 792,865 + +1.6 792,865 + -0.6 792,865 + +2.0 792,865 + -1.8 792,873 - +1.1 792,873 - -0.4 792,887 + +0.7 792,929 - +3.8 792,929 - -0.3 792,931 - -1.8 792,999 + +0.0 792,999 + -1.4 792,999 + -0.8 792,999 + -0.8 792,999 + -0.8 792,999 + +3.5 793,006 - +0.2 793,007 - -0.4 793,007 - -0.8 793,007 - -0.7 793,022 - -0.3 793,048 + +1.1 793,050 + +2.0 793,050 + +0.2 793,096 + +1.0 793,106 + +1.5 793,114 - +0.1 793,114 - -0.3 793,116 + +4.3 793,121 + +2.1 793,124 - -0.1 793,129 + +2.9 793,129 + +2.7 793,131 + +0.5 793,158 + -1.7 793,262 + -1.5 793,262 + +0.7 793,270 - +2.6 793,274 + +2.9 793,312 + +3.4 793,325 - -1.0 793,325 - +0.2 793,325 - +2.4 793,325 - -0.7 793,352 + +2.0 793,369 + +2.6 793,418 - -0.4 793,488 + BBR_RS13460 0.12 -0.4 793,597 - BBR_RS13460 0.21 +0.5 793,598 + BBR_RS13460 0.21 +0.2 793,599 - BBR_RS13460 0.21 +2.3 793,614 + BBR_RS13460 0.22 +1.4 793,616 - BBR_RS13460 0.22 +1.1 793,761 - BBR_RS13460 0.34 +1.6 793,821 + BBR_RS13460 0.38 +2.3 793,829 - BBR_RS13460 0.39 +0.1 793,835 + BBR_RS13460 0.40 -1.1 793,843 - BBR_RS13460 0.40 +2.0 793,843 - BBR_RS13460 0.40 -0.8 793,890 - BBR_RS13460 0.44 -0.3 793,899 + BBR_RS13460 0.45 +4.5 793,907 - BBR_RS13460 0.45 +2.3 793,907 - BBR_RS13460 0.45 +3.9 793,909 + BBR_RS13460 0.45 -0.2 793,909 + BBR_RS13460 0.45 +2.6 793,924 + BBR_RS13460 0.47 +4.0 793,924 + BBR_RS13460 0.47 +3.1 793,939 - BBR_RS13460 0.48 +2.5 793,947 - BBR_RS13460 0.49 -2.5 793,953 + BBR_RS13460 0.49 +2.7 793,971 + BBR_RS13460 0.50 +1.2 794,017 - BBR_RS13460 0.54 +1.1 794,061 + BBR_RS13460 0.58 +0.1 794,094 - BBR_RS13460 0.60 -1.3 794,117 + BBR_RS13460 0.62 -1.9 794,126 + BBR_RS13460 0.63 +1.1 794,126 - BBR_RS13460 0.63 -3.3 794,128 + BBR_RS13460 0.63 -1.5 794,128 + BBR_RS13460 0.63 +1.0 794,128 + BBR_RS13460 0.63 -1.1 794,128 + BBR_RS13460 0.63 +3.0 794,128 + BBR_RS13460 0.63 -1.3 794,128 + BBR_RS13460 0.63 -0.5 794,128 + BBR_RS13460 0.63 +3.7 794,128 + BBR_RS13460 0.63 +0.5 794,128 + BBR_RS13460 0.63 -1.1 794,128 + BBR_RS13460 0.63 +1.9 794,128 + BBR_RS13460 0.63 +0.6 794,128 + BBR_RS13460 0.63 -1.5 794,128 + BBR_RS13460 0.63 +1.8 794,128 + BBR_RS13460 0.63 +1.8 794,128 + BBR_RS13460 0.63 +3.9 794,128 + BBR_RS13460 0.63 +1.4 794,133 + BBR_RS13460 0.63 +0.7 794,135 + BBR_RS13460 0.64 -1.0 794,136 - BBR_RS13460 0.64 +0.9 794,136 - BBR_RS13460 0.64 +1.4 794,136 - BBR_RS13460 0.64 -0.5 794,136 - BBR_RS13460 0.64 +1.7 794,136 - BBR_RS13460 0.64 -1.6 794,136 - BBR_RS13460 0.64 +0.2 794,136 - BBR_RS13460 0.64 +0.0 794,136 - BBR_RS13460 0.64 +1.0 794,136 - BBR_RS13460 0.64 +2.7 794,136 - BBR_RS13460 0.64 +1.8 794,136 - BBR_RS13460 0.64 -0.8 794,136 - BBR_RS13460 0.64 -0.9 794,136 - BBR_RS13460 0.64 +1.7 794,136 - BBR_RS13460 0.64 +3.8 794,143 - BBR_RS13460 0.64 -2.0 794,197 + BBR_RS13460 0.69 -0.1 794,207 + BBR_RS13460 0.69 -0.1 794,212 + BBR_RS13460 0.70 +2.9 794,215 - BBR_RS13460 0.70 -1.4 794,234 + BBR_RS13460 0.71 +0.2 794,259 - BBR_RS13460 0.73 -0.6 794,271 + BBR_RS13460 0.74 -1.2 794,271 + BBR_RS13460 0.74 -1.0 794,307 + BBR_RS13460 0.77 -1.8 794,311 - BBR_RS13460 0.78 +2.7 794,336 + BBR_RS13460 0.80 +2.7 794,352 + BBR_RS13460 0.81 +0.3 794,352 - BBR_RS13460 0.81 -1.0 794,382 + BBR_RS13460 0.83 -1.4 794,383 + BBR_RS13460 0.83 -1.0 794,383 + BBR_RS13460 0.83 -0.6 794,388 + BBR_RS13460 0.84 +1.9 794,391 - BBR_RS13460 0.84 -0.8 794,391 - BBR_RS13460 0.84 +1.0 794,391 - BBR_RS13460 0.84 -0.2 794,408 - BBR_RS13460 0.85 +3.1 794,452 + BBR_RS13460 0.89 +0.0 794,498 + +2.0 794,498 + +0.1 794,571 - -2.4 794,584 - -0.5 794,584 - +0.7 794,679 + +2.7 794,687 - -1.6 794,819 - +0.4 794,824 - BBR_RS13465 0.10 +0.2 794,840 + BBR_RS13465 0.11 +0.3 794,860 + BBR_RS13465 0.13 +1.3 794,874 + BBR_RS13465 0.14 +1.3 794,929 - BBR_RS13465 0.19 -0.1 794,940 - BBR_RS13465 0.20 +1.0 794,972 - BBR_RS13465 0.23 -1.7 795,008 - BBR_RS13465 0.26 -2.7 795,107 - BBR_RS13465 0.34 -0.7 795,110 - BBR_RS13465 0.35 -1.0 795,253 - BBR_RS13465 0.47 -1.3 795,316 - BBR_RS13465 0.52 -1.2 795,316 - BBR_RS13465 0.52 -0.3 795,365 - BBR_RS13465 0.57 -0.4 795,377 + BBR_RS13465 0.58 +1.1 795,387 + BBR_RS13465 0.59 +1.3 795,387 + BBR_RS13465 0.59 -0.9 795,387 + BBR_RS13465 0.59 -0.3 795,387 + BBR_RS13465 0.59 -0.6 795,387 + BBR_RS13465 0.59 -0.9 795,395 - BBR_RS13465 0.59 -0.3 795,395 - BBR_RS13465 0.59 +2.4 795,395 - BBR_RS13465 0.59 -0.3 795,395 - BBR_RS13465 0.59 +2.3 795,395 - BBR_RS13465 0.59 +2.6 795,395 - BBR_RS13465 0.59 -1.1 795,395 - BBR_RS13465 0.59 +0.6 795,395 - BBR_RS13465 0.59 -0.3 795,395 - BBR_RS13465 0.59 +2.0 795,443 + BBR_RS13465 0.63 -0.8 795,510 - BBR_RS13465 0.69 +0.2 795,554 - BBR_RS13465 0.73 -0.5 795,554 - BBR_RS13465 0.73 -1.1 795,561 + BBR_RS13465 0.74 +0.2
Or see this region's nucleotide sequence