Experiment: reconstituted MRS with Difucosyllactose 7.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13275 and BBR_RS13280 are separated by 11 nucleotides BBR_RS13280 and BBR_RS13285 overlap by 17 nucleotides
BBR_RS13275: BBR_RS13275 - hypothetical protein, at 752,426 to 753,721
_RS13275
BBR_RS13280: BBR_RS13280 - ABC transporter permease, at 753,733 to 755,262
_RS13280
BBR_RS13285: BBR_RS13285 - 1-deoxy-D-xylulose-5-phosphate synthase, at 755,246 to 757,297
_RS13285
Position (kb) 753
754
755
756 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 752.783 kb on + strand, within BBR_RS13275 at 752.790 kb on - strand, within BBR_RS13275 at 752.811 kb on - strand, within BBR_RS13275 at 752.813 kb on + strand, within BBR_RS13275 at 752.813 kb on - strand, within BBR_RS13275 at 752.821 kb on - strand, within BBR_RS13275 at 752.844 kb on - strand, within BBR_RS13275 at 752.850 kb on - strand, within BBR_RS13275 at 752.852 kb on + strand, within BBR_RS13275 at 752.912 kb on - strand, within BBR_RS13275 at 752.912 kb on - strand, within BBR_RS13275 at 752.984 kb on + strand, within BBR_RS13275 at 752.999 kb on + strand, within BBR_RS13275 at 753.022 kb on + strand, within BBR_RS13275 at 753.037 kb on + strand, within BBR_RS13275 at 753.045 kb on - strand, within BBR_RS13275 at 753.082 kb on + strand, within BBR_RS13275 at 753.082 kb on + strand, within BBR_RS13275 at 753.097 kb on + strand, within BBR_RS13275 at 753.105 kb on + strand, within BBR_RS13275 at 753.109 kb on + strand, within BBR_RS13275 at 753.157 kb on - strand, within BBR_RS13275 at 753.194 kb on + strand, within BBR_RS13275 at 753.200 kb on - strand, within BBR_RS13275 at 753.204 kb on + strand, within BBR_RS13275 at 753.204 kb on + strand, within BBR_RS13275 at 753.212 kb on - strand, within BBR_RS13275 at 753.216 kb on + strand, within BBR_RS13275 at 753.226 kb on + strand, within BBR_RS13275 at 753.249 kb on - strand, within BBR_RS13275 at 753.292 kb on - strand, within BBR_RS13275 at 753.294 kb on - strand, within BBR_RS13275 at 753.350 kb on - strand, within BBR_RS13275 at 753.375 kb on + strand, within BBR_RS13275 at 753.517 kb on + strand, within BBR_RS13275 at 753.517 kb on + strand, within BBR_RS13275 at 753.517 kb on + strand, within BBR_RS13275 at 753.517 kb on + strand, within BBR_RS13275 at 753.620 kb on + strand at 753.620 kb on + strand at 753.757 kb on - strand at 753.763 kb on + strand at 753.789 kb on - strand at 753.791 kb on + strand at 753.804 kb on - strand at 753.804 kb on - strand at 753.805 kb on + strand at 753.828 kb on + strand at 753.830 kb on + strand at 753.861 kb on + strand at 753.922 kb on + strand, within BBR_RS13280 at 754.091 kb on - strand, within BBR_RS13280 at 754.097 kb on - strand, within BBR_RS13280 at 754.231 kb on + strand, within BBR_RS13280 at 754.239 kb on - strand, within BBR_RS13280 at 754.254 kb on + strand, within BBR_RS13280 at 754.307 kb on - strand, within BBR_RS13280 at 754.415 kb on + strand, within BBR_RS13280 at 754.487 kb on + strand, within BBR_RS13280 at 754.542 kb on - strand, within BBR_RS13280 at 754.565 kb on - strand, within BBR_RS13280 at 754.607 kb on - strand, within BBR_RS13280 at 754.644 kb on + strand, within BBR_RS13280 at 754.659 kb on + strand, within BBR_RS13280 at 754.667 kb on - strand, within BBR_RS13280 at 754.829 kb on - strand, within BBR_RS13280 at 754.833 kb on + strand, within BBR_RS13280 at 754.969 kb on - strand, within BBR_RS13280 at 755.081 kb on + strand, within BBR_RS13280 at 755.134 kb on + strand at 755.164 kb on + strand at 755.164 kb on + strand at 755.174 kb on - strand at 755.198 kb on + strand at 755.320 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM remove 752,783 + BBR_RS13275 0.28 -3.5 752,790 - BBR_RS13275 0.28 -1.0 752,811 - BBR_RS13275 0.30 +0.1 752,813 + BBR_RS13275 0.30 -1.4 752,813 - BBR_RS13275 0.30 -1.2 752,821 - BBR_RS13275 0.30 +0.0 752,844 - BBR_RS13275 0.32 +1.7 752,850 - BBR_RS13275 0.33 +0.0 752,852 + BBR_RS13275 0.33 -0.1 752,912 - BBR_RS13275 0.38 -0.7 752,912 - BBR_RS13275 0.38 +0.3 752,984 + BBR_RS13275 0.43 +4.1 752,999 + BBR_RS13275 0.44 +3.3 753,022 + BBR_RS13275 0.46 +0.7 753,037 + BBR_RS13275 0.47 +3.0 753,045 - BBR_RS13275 0.48 -1.2 753,082 + BBR_RS13275 0.51 -0.6 753,082 + BBR_RS13275 0.51 +3.9 753,097 + BBR_RS13275 0.52 +2.2 753,105 + BBR_RS13275 0.52 +1.3 753,109 + BBR_RS13275 0.53 -0.3 753,157 - BBR_RS13275 0.56 -0.8 753,194 + BBR_RS13275 0.59 +2.7 753,200 - BBR_RS13275 0.60 -1.8 753,204 + BBR_RS13275 0.60 +3.5 753,204 + BBR_RS13275 0.60 +2.6 753,212 - BBR_RS13275 0.61 -1.2 753,216 + BBR_RS13275 0.61 -2.1 753,226 + BBR_RS13275 0.62 +1.6 753,249 - BBR_RS13275 0.64 -0.3 753,292 - BBR_RS13275 0.67 +0.5 753,294 - BBR_RS13275 0.67 -1.2 753,350 - BBR_RS13275 0.71 +3.8 753,375 + BBR_RS13275 0.73 -1.3 753,517 + BBR_RS13275 0.84 -0.7 753,517 + BBR_RS13275 0.84 -1.3 753,517 + BBR_RS13275 0.84 -0.8 753,517 + BBR_RS13275 0.84 +1.7 753,620 + +3.0 753,620 + -1.2 753,757 - +2.1 753,763 + -1.2 753,789 - -1.7 753,791 + -1.6 753,804 - +1.0 753,804 - +1.5 753,805 + +2.2 753,828 + +2.4 753,830 + +1.2 753,861 + -1.7 753,922 + BBR_RS13280 0.12 -0.4 754,091 - BBR_RS13280 0.23 +0.2 754,097 - BBR_RS13280 0.24 +3.2 754,231 + BBR_RS13280 0.33 +0.2 754,239 - BBR_RS13280 0.33 -1.1 754,254 + BBR_RS13280 0.34 -0.9 754,307 - BBR_RS13280 0.38 -1.5 754,415 + BBR_RS13280 0.45 -0.1 754,487 + BBR_RS13280 0.49 -0.6 754,542 - BBR_RS13280 0.53 -1.2 754,565 - BBR_RS13280 0.54 -1.5 754,607 - BBR_RS13280 0.57 +1.3 754,644 + BBR_RS13280 0.60 -1.8 754,659 + BBR_RS13280 0.61 -1.1 754,667 - BBR_RS13280 0.61 +2.0 754,829 - BBR_RS13280 0.72 -0.1 754,833 + BBR_RS13280 0.72 +1.9 754,969 - BBR_RS13280 0.81 +0.3 755,081 + BBR_RS13280 0.88 -2.2 755,134 + +1.9 755,164 + +0.9 755,164 + -1.6 755,174 - -1.9 755,198 + +2.8 755,320 - -0.2
Or see this region's nucleotide sequence