Experiment: reconstituted MRS with Difucosyllactose 7.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13240 and BBR_RS13245 are separated by 11 nucleotides BBR_RS13245 and BBR_RS13250 overlap by 20 nucleotides
BBR_RS13240: BBR_RS13240 - sugar ABC transporter substrate-binding protein, at 743,899 to 745,230
_RS13240
BBR_RS13245: BBR_RS13245 - LacI family transcriptional regulator, at 745,242 to 746,486
_RS13245
BBR_RS13250: BBR_RS13250 - alpha-glucosidase, at 746,467 to 748,281
_RS13250
Position (kb) 745
746
747 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 744.245 kb on + strand, within BBR_RS13240 at 744.245 kb on + strand, within BBR_RS13240 at 744.246 kb on + strand, within BBR_RS13240 at 744.254 kb on - strand, within BBR_RS13240 at 744.255 kb on + strand, within BBR_RS13240 at 744.265 kb on - strand, within BBR_RS13240 at 744.301 kb on + strand, within BBR_RS13240 at 744.378 kb on - strand, within BBR_RS13240 at 744.402 kb on + strand, within BBR_RS13240 at 744.402 kb on + strand, within BBR_RS13240 at 744.481 kb on + strand, within BBR_RS13240 at 744.486 kb on + strand, within BBR_RS13240 at 744.497 kb on + strand, within BBR_RS13240 at 744.507 kb on + strand, within BBR_RS13240 at 744.507 kb on + strand, within BBR_RS13240 at 744.507 kb on + strand, within BBR_RS13240 at 744.526 kb on - strand, within BBR_RS13240 at 744.536 kb on - strand, within BBR_RS13240 at 744.536 kb on - strand, within BBR_RS13240 at 744.554 kb on + strand, within BBR_RS13240 at 744.605 kb on + strand, within BBR_RS13240 at 744.612 kb on + strand, within BBR_RS13240 at 744.636 kb on - strand, within BBR_RS13240 at 744.646 kb on + strand, within BBR_RS13240 at 744.646 kb on + strand, within BBR_RS13240 at 744.714 kb on - strand, within BBR_RS13240 at 744.730 kb on + strand, within BBR_RS13240 at 744.785 kb on + strand, within BBR_RS13240 at 744.791 kb on + strand, within BBR_RS13240 at 744.793 kb on + strand, within BBR_RS13240 at 744.793 kb on - strand, within BBR_RS13240 at 744.801 kb on - strand, within BBR_RS13240 at 744.833 kb on - strand, within BBR_RS13240 at 744.870 kb on - strand, within BBR_RS13240 at 744.895 kb on + strand, within BBR_RS13240 at 744.905 kb on + strand, within BBR_RS13240 at 744.967 kb on - strand, within BBR_RS13240 at 744.967 kb on - strand, within BBR_RS13240 at 744.967 kb on - strand, within BBR_RS13240 at 744.982 kb on - strand, within BBR_RS13240 at 744.987 kb on - strand, within BBR_RS13240 at 745.008 kb on - strand, within BBR_RS13240 at 745.129 kb on + strand at 745.129 kb on + strand at 745.139 kb on - strand at 745.146 kb on - strand at 745.146 kb on - strand at 745.146 kb on - strand at 745.146 kb on - strand at 745.146 kb on - strand at 745.160 kb on + strand at 745.161 kb on + strand at 745.169 kb on - strand at 745.174 kb on - strand at 745.194 kb on + strand at 745.194 kb on + strand at 745.201 kb on + strand at 745.202 kb on - strand at 745.202 kb on - strand at 745.206 kb on + strand at 745.206 kb on + strand at 745.234 kb on + strand at 745.273 kb on - strand at 745.355 kb on - strand at 745.357 kb on + strand at 745.410 kb on + strand, within BBR_RS13245 at 745.428 kb on + strand, within BBR_RS13245 at 745.428 kb on + strand, within BBR_RS13245 at 745.429 kb on - strand, within BBR_RS13245 at 745.436 kb on - strand, within BBR_RS13245 at 745.441 kb on - strand, within BBR_RS13245 at 745.488 kb on - strand, within BBR_RS13245 at 745.492 kb on + strand, within BBR_RS13245 at 745.492 kb on + strand, within BBR_RS13245 at 745.492 kb on + strand, within BBR_RS13245 at 745.493 kb on + strand, within BBR_RS13245 at 745.501 kb on - strand, within BBR_RS13245 at 745.525 kb on + strand, within BBR_RS13245 at 745.527 kb on + strand, within BBR_RS13245 at 745.562 kb on - strand, within BBR_RS13245 at 745.584 kb on + strand, within BBR_RS13245 at 745.592 kb on - strand, within BBR_RS13245 at 745.594 kb on - strand, within BBR_RS13245 at 745.594 kb on - strand, within BBR_RS13245 at 745.602 kb on - strand, within BBR_RS13245 at 745.607 kb on - strand, within BBR_RS13245 at 745.620 kb on + strand, within BBR_RS13245 at 745.628 kb on - strand, within BBR_RS13245 at 745.674 kb on + strand, within BBR_RS13245 at 745.725 kb on - strand, within BBR_RS13245 at 745.759 kb on + strand, within BBR_RS13245 at 745.769 kb on + strand, within BBR_RS13245 at 745.777 kb on - strand, within BBR_RS13245 at 745.777 kb on - strand, within BBR_RS13245 at 745.779 kb on + strand, within BBR_RS13245 at 745.779 kb on + strand, within BBR_RS13245 at 745.829 kb on + strand, within BBR_RS13245 at 745.854 kb on + strand, within BBR_RS13245 at 745.862 kb on - strand, within BBR_RS13245 at 745.879 kb on + strand, within BBR_RS13245 at 745.893 kb on + strand, within BBR_RS13245 at 745.952 kb on + strand, within BBR_RS13245 at 745.952 kb on + strand, within BBR_RS13245 at 745.953 kb on + strand, within BBR_RS13245 at 745.960 kb on - strand, within BBR_RS13245 at 745.972 kb on - strand, within BBR_RS13245 at 745.976 kb on + strand, within BBR_RS13245 at 745.994 kb on + strand, within BBR_RS13245 at 746.021 kb on - strand, within BBR_RS13245 at 746.049 kb on + strand, within BBR_RS13245 at 746.077 kb on + strand, within BBR_RS13245 at 746.128 kb on + strand, within BBR_RS13245 at 746.133 kb on + strand, within BBR_RS13245 at 746.148 kb on + strand, within BBR_RS13245 at 746.157 kb on - strand, within BBR_RS13245 at 746.210 kb on + strand, within BBR_RS13245 at 746.210 kb on + strand, within BBR_RS13245 at 746.223 kb on + strand, within BBR_RS13245 at 746.238 kb on - strand, within BBR_RS13245 at 746.259 kb on + strand, within BBR_RS13245 at 746.290 kb on - strand, within BBR_RS13245 at 746.290 kb on - strand, within BBR_RS13245 at 746.290 kb on - strand, within BBR_RS13245 at 746.308 kb on + strand, within BBR_RS13245 at 746.364 kb on + strand at 746.398 kb on + strand at 746.458 kb on - strand at 746.466 kb on + strand at 746.466 kb on + strand at 746.481 kb on - strand at 746.488 kb on + strand at 746.488 kb on + strand at 746.496 kb on - strand at 746.530 kb on - strand at 746.553 kb on + strand at 746.564 kb on + strand at 746.575 kb on + strand at 746.608 kb on - strand at 746.656 kb on + strand, within BBR_RS13250 at 746.661 kb on + strand, within BBR_RS13250 at 746.661 kb on + strand, within BBR_RS13250 at 746.669 kb on - strand, within BBR_RS13250 at 746.674 kb on - strand, within BBR_RS13250 at 746.677 kb on + strand, within BBR_RS13250 at 746.677 kb on + strand, within BBR_RS13250 at 746.677 kb on + strand, within BBR_RS13250 at 746.679 kb on + strand, within BBR_RS13250 at 746.679 kb on + strand, within BBR_RS13250 at 746.685 kb on - strand, within BBR_RS13250 at 746.685 kb on - strand, within BBR_RS13250 at 746.685 kb on - strand, within BBR_RS13250 at 746.712 kb on - strand, within BBR_RS13250 at 746.758 kb on - strand, within BBR_RS13250 at 746.770 kb on - strand, within BBR_RS13250 at 746.792 kb on - strand, within BBR_RS13250 at 746.792 kb on - strand, within BBR_RS13250 at 746.832 kb on - strand, within BBR_RS13250 at 746.865 kb on + strand, within BBR_RS13250 at 746.865 kb on + strand, within BBR_RS13250 at 746.873 kb on - strand, within BBR_RS13250 at 746.887 kb on + strand, within BBR_RS13250 at 746.895 kb on - strand, within BBR_RS13250 at 746.899 kb on - strand, within BBR_RS13250 at 746.899 kb on - strand, within BBR_RS13250 at 746.939 kb on + strand, within BBR_RS13250 at 746.959 kb on + strand, within BBR_RS13250 at 746.985 kb on + strand, within BBR_RS13250 at 746.993 kb on + strand, within BBR_RS13250 at 746.993 kb on - strand, within BBR_RS13250 at 747.015 kb on - strand, within BBR_RS13250 at 747.022 kb on - strand, within BBR_RS13250 at 747.043 kb on + strand, within BBR_RS13250 at 747.051 kb on - strand, within BBR_RS13250 at 747.072 kb on - strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.079 kb on + strand, within BBR_RS13250 at 747.087 kb on - strand, within BBR_RS13250 at 747.131 kb on - strand, within BBR_RS13250 at 747.142 kb on + strand, within BBR_RS13250 at 747.145 kb on - strand, within BBR_RS13250 at 747.184 kb on + strand, within BBR_RS13250 at 747.194 kb on + strand, within BBR_RS13250 at 747.228 kb on + strand, within BBR_RS13250 at 747.228 kb on + strand, within BBR_RS13250 at 747.228 kb on + strand, within BBR_RS13250 at 747.231 kb on - strand, within BBR_RS13250 at 747.233 kb on + strand, within BBR_RS13250 at 747.236 kb on - strand, within BBR_RS13250 at 747.236 kb on - strand, within BBR_RS13250 at 747.236 kb on - strand, within BBR_RS13250 at 747.237 kb on - strand, within BBR_RS13250 at 747.252 kb on - strand, within BBR_RS13250 at 747.252 kb on - strand, within BBR_RS13250 at 747.257 kb on - strand, within BBR_RS13250 at 747.263 kb on + strand, within BBR_RS13250 at 747.322 kb on - strand, within BBR_RS13250 at 747.408 kb on + strand, within BBR_RS13250 at 747.418 kb on + strand, within BBR_RS13250 at 747.432 kb on - strand, within BBR_RS13250
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM remove 744,245 + BBR_RS13240 0.26 +2.0 744,245 + BBR_RS13240 0.26 +2.4 744,246 + BBR_RS13240 0.26 +3.4 744,254 - BBR_RS13240 0.27 -0.2 744,255 + BBR_RS13240 0.27 +2.2 744,265 - BBR_RS13240 0.27 -1.2 744,301 + BBR_RS13240 0.30 +1.6 744,378 - BBR_RS13240 0.36 -0.3 744,402 + BBR_RS13240 0.38 +4.0 744,402 + BBR_RS13240 0.38 +3.0 744,481 + BBR_RS13240 0.44 +1.7 744,486 + BBR_RS13240 0.44 -0.6 744,497 + BBR_RS13240 0.45 -2.8 744,507 + BBR_RS13240 0.46 -1.6 744,507 + BBR_RS13240 0.46 -0.4 744,507 + BBR_RS13240 0.46 +2.3 744,526 - BBR_RS13240 0.47 -0.7 744,536 - BBR_RS13240 0.48 +0.9 744,536 - BBR_RS13240 0.48 -0.6 744,554 + BBR_RS13240 0.49 -1.7 744,605 + BBR_RS13240 0.53 +2.6 744,612 + BBR_RS13240 0.54 +4.0 744,636 - BBR_RS13240 0.55 -0.7 744,646 + BBR_RS13240 0.56 +0.3 744,646 + BBR_RS13240 0.56 +1.5 744,714 - BBR_RS13240 0.61 +2.1 744,730 + BBR_RS13240 0.62 -0.9 744,785 + BBR_RS13240 0.67 +0.2 744,791 + BBR_RS13240 0.67 +0.3 744,793 + BBR_RS13240 0.67 +1.8 744,793 - BBR_RS13240 0.67 -1.6 744,801 - BBR_RS13240 0.68 -1.3 744,833 - BBR_RS13240 0.70 -1.0 744,870 - BBR_RS13240 0.73 -0.1 744,895 + BBR_RS13240 0.75 +0.5 744,905 + BBR_RS13240 0.76 +0.1 744,967 - BBR_RS13240 0.80 +3.3 744,967 - BBR_RS13240 0.80 -1.4 744,967 - BBR_RS13240 0.80 -0.5 744,982 - BBR_RS13240 0.81 +1.4 744,987 - BBR_RS13240 0.82 +2.5 745,008 - BBR_RS13240 0.83 +3.3 745,129 + -1.6 745,129 + -1.9 745,139 - +2.2 745,146 - -0.9 745,146 - +0.4 745,146 - +0.5 745,146 - -0.6 745,146 - -1.6 745,160 + +0.0 745,161 + -1.4 745,169 - -0.8 745,174 - -0.3 745,194 + -1.4 745,194 + +2.5 745,201 + +2.1 745,202 - -1.0 745,202 - -0.4 745,206 + +1.0 745,206 + -0.9 745,234 + -0.7 745,273 - +1.2 745,355 - +2.3 745,357 + -0.9 745,410 + BBR_RS13245 0.13 +1.6 745,428 + BBR_RS13245 0.15 +1.3 745,428 + BBR_RS13245 0.15 +0.1 745,429 - BBR_RS13245 0.15 -1.6 745,436 - BBR_RS13245 0.16 -0.9 745,441 - BBR_RS13245 0.16 -1.2 745,488 - BBR_RS13245 0.20 -1.6 745,492 + BBR_RS13245 0.20 +0.7 745,492 + BBR_RS13245 0.20 +1.5 745,492 + BBR_RS13245 0.20 +1.3 745,493 + BBR_RS13245 0.20 -0.7 745,501 - BBR_RS13245 0.21 +3.6 745,525 + BBR_RS13245 0.23 +1.1 745,527 + BBR_RS13245 0.23 +2.0 745,562 - BBR_RS13245 0.26 +0.5 745,584 + BBR_RS13245 0.27 -1.4 745,592 - BBR_RS13245 0.28 +1.7 745,594 - BBR_RS13245 0.28 +1.1 745,594 - BBR_RS13245 0.28 -1.7 745,602 - BBR_RS13245 0.29 -0.9 745,607 - BBR_RS13245 0.29 +1.9 745,620 + BBR_RS13245 0.30 -0.8 745,628 - BBR_RS13245 0.31 -0.9 745,674 + BBR_RS13245 0.35 -1.4 745,725 - BBR_RS13245 0.39 -1.0 745,759 + BBR_RS13245 0.42 -1.8 745,769 + BBR_RS13245 0.42 -1.0 745,777 - BBR_RS13245 0.43 -0.7 745,777 - BBR_RS13245 0.43 +2.1 745,779 + BBR_RS13245 0.43 -2.2 745,779 + BBR_RS13245 0.43 +2.4 745,829 + BBR_RS13245 0.47 +0.8 745,854 + BBR_RS13245 0.49 -0.2 745,862 - BBR_RS13245 0.50 +0.5 745,879 + BBR_RS13245 0.51 +1.4 745,893 + BBR_RS13245 0.52 -1.7 745,952 + BBR_RS13245 0.57 -1.8 745,952 + BBR_RS13245 0.57 +1.8 745,953 + BBR_RS13245 0.57 -0.2 745,960 - BBR_RS13245 0.58 +2.2 745,972 - BBR_RS13245 0.59 -1.6 745,976 + BBR_RS13245 0.59 -0.8 745,994 + BBR_RS13245 0.60 -0.7 746,021 - BBR_RS13245 0.63 -1.1 746,049 + BBR_RS13245 0.65 -1.2 746,077 + BBR_RS13245 0.67 -1.7 746,128 + BBR_RS13245 0.71 +2.1 746,133 + BBR_RS13245 0.72 -0.5 746,148 + BBR_RS13245 0.73 -1.2 746,157 - BBR_RS13245 0.73 -1.2 746,210 + BBR_RS13245 0.78 -0.0 746,210 + BBR_RS13245 0.78 -1.0 746,223 + BBR_RS13245 0.79 -1.8 746,238 - BBR_RS13245 0.80 -1.1 746,259 + BBR_RS13245 0.82 -1.5 746,290 - BBR_RS13245 0.84 +0.0 746,290 - BBR_RS13245 0.84 +1.9 746,290 - BBR_RS13245 0.84 -0.3 746,308 + BBR_RS13245 0.86 +0.2 746,364 + +0.2 746,398 + +1.5 746,458 - -0.6 746,466 + +3.0 746,466 + -1.4 746,481 - +3.5 746,488 + +3.0 746,488 + +0.2 746,496 - -0.4 746,530 - +2.0 746,553 + +2.0 746,564 + +1.0 746,575 + -1.7 746,608 - +0.8 746,656 + BBR_RS13250 0.10 -1.1 746,661 + BBR_RS13250 0.11 -1.1 746,661 + BBR_RS13250 0.11 +2.5 746,669 - BBR_RS13250 0.11 -1.5 746,674 - BBR_RS13250 0.11 -2.3 746,677 + BBR_RS13250 0.12 -0.4 746,677 + BBR_RS13250 0.12 -0.1 746,677 + BBR_RS13250 0.12 -1.0 746,679 + BBR_RS13250 0.12 -1.4 746,679 + BBR_RS13250 0.12 -1.0 746,685 - BBR_RS13250 0.12 +0.7 746,685 - BBR_RS13250 0.12 +0.3 746,685 - BBR_RS13250 0.12 +4.0 746,712 - BBR_RS13250 0.13 +2.0 746,758 - BBR_RS13250 0.16 -1.8 746,770 - BBR_RS13250 0.17 +1.2 746,792 - BBR_RS13250 0.18 -1.7 746,792 - BBR_RS13250 0.18 +1.0 746,832 - BBR_RS13250 0.20 -0.8 746,865 + BBR_RS13250 0.22 -1.2 746,865 + BBR_RS13250 0.22 -1.4 746,873 - BBR_RS13250 0.22 +3.8 746,887 + BBR_RS13250 0.23 -0.3 746,895 - BBR_RS13250 0.24 +1.3 746,899 - BBR_RS13250 0.24 -0.3 746,899 - BBR_RS13250 0.24 -1.2 746,939 + BBR_RS13250 0.26 +2.6 746,959 + BBR_RS13250 0.27 -1.0 746,985 + BBR_RS13250 0.29 +1.7 746,993 + BBR_RS13250 0.29 +0.1 746,993 - BBR_RS13250 0.29 +2.2 747,015 - BBR_RS13250 0.30 -1.3 747,022 - BBR_RS13250 0.31 -1.7 747,043 + BBR_RS13250 0.32 +2.3 747,051 - BBR_RS13250 0.32 +1.4 747,072 - BBR_RS13250 0.33 -0.5 747,079 + BBR_RS13250 0.34 -0.6 747,079 + BBR_RS13250 0.34 +0.4 747,079 + BBR_RS13250 0.34 -1.1 747,079 + BBR_RS13250 0.34 -1.5 747,079 + BBR_RS13250 0.34 -1.4 747,079 + BBR_RS13250 0.34 +0.5 747,079 + BBR_RS13250 0.34 -1.3 747,087 - BBR_RS13250 0.34 -1.1 747,131 - BBR_RS13250 0.37 -2.0 747,142 + BBR_RS13250 0.37 +2.1 747,145 - BBR_RS13250 0.37 +2.0 747,184 + BBR_RS13250 0.40 -0.1 747,194 + BBR_RS13250 0.40 +1.1 747,228 + BBR_RS13250 0.42 +1.6 747,228 + BBR_RS13250 0.42 +2.8 747,228 + BBR_RS13250 0.42 +1.1 747,231 - BBR_RS13250 0.42 -0.4 747,233 + BBR_RS13250 0.42 -0.2 747,236 - BBR_RS13250 0.42 +1.0 747,236 - BBR_RS13250 0.42 +2.7 747,236 - BBR_RS13250 0.42 +2.9 747,237 - BBR_RS13250 0.42 -1.8 747,252 - BBR_RS13250 0.43 -1.2 747,252 - BBR_RS13250 0.43 -1.7 747,257 - BBR_RS13250 0.44 +1.9 747,263 + BBR_RS13250 0.44 -0.3 747,322 - BBR_RS13250 0.47 +0.3 747,408 + BBR_RS13250 0.52 +2.3 747,418 + BBR_RS13250 0.52 -0.2 747,432 - BBR_RS13250 0.53 +0.0
Or see this region's nucleotide sequence