Experiment: reconstituted MRS with Difucosyllactose 7.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12320 and BBR_RS12325 are separated by 210 nucleotides BBR_RS12325 and BBR_RS12330 are separated by 234 nucleotides
BBR_RS12320: BBR_RS12320 - cysteine--tRNA ligase, at 515,408 to 517,057
_RS12320
BBR_RS12325: BBR_RS12325 - type 1 glutamine amidotransferase, at 517,268 to 517,996
_RS12325
BBR_RS12330: BBR_RS12330 - ABC transporter ATP-binding protein, at 518,231 to 520,444
_RS12330
Position (kb) 517
518 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 517.070 kb on - strand at 517.081 kb on - strand at 517.086 kb on + strand at 517.094 kb on - strand at 517.094 kb on - strand at 517.094 kb on - strand at 517.094 kb on - strand at 517.124 kb on + strand at 517.134 kb on - strand at 517.142 kb on - strand at 517.142 kb on - strand at 517.142 kb on - strand at 517.142 kb on - strand at 517.156 kb on + strand at 517.165 kb on - strand at 517.234 kb on - strand at 517.255 kb on + strand at 517.265 kb on - strand at 517.275 kb on + strand at 517.300 kb on + strand at 517.300 kb on + strand at 517.399 kb on + strand, within BBR_RS12325 at 517.415 kb on - strand, within BBR_RS12325 at 517.429 kb on + strand, within BBR_RS12325 at 517.429 kb on + strand, within BBR_RS12325 at 517.437 kb on - strand, within BBR_RS12325 at 517.475 kb on + strand, within BBR_RS12325 at 517.508 kb on + strand, within BBR_RS12325 at 517.508 kb on + strand, within BBR_RS12325 at 517.585 kb on + strand, within BBR_RS12325 at 517.585 kb on + strand, within BBR_RS12325 at 517.585 kb on + strand, within BBR_RS12325 at 517.642 kb on - strand, within BBR_RS12325 at 517.646 kb on - strand, within BBR_RS12325 at 517.708 kb on + strand, within BBR_RS12325 at 517.712 kb on - strand, within BBR_RS12325 at 517.758 kb on - strand, within BBR_RS12325 at 517.758 kb on - strand, within BBR_RS12325 at 517.768 kb on + strand, within BBR_RS12325 at 517.770 kb on + strand, within BBR_RS12325 at 517.802 kb on - strand, within BBR_RS12325 at 517.804 kb on + strand, within BBR_RS12325 at 517.804 kb on + strand, within BBR_RS12325 at 517.804 kb on + strand, within BBR_RS12325 at 517.863 kb on - strand, within BBR_RS12325 at 517.863 kb on - strand, within BBR_RS12325 at 517.873 kb on + strand, within BBR_RS12325 at 517.974 kb on + strand at 517.987 kb on + strand at 518.052 kb on + strand at 518.131 kb on - strand at 518.147 kb on - strand at 518.165 kb on + strand at 518.185 kb on + strand at 518.200 kb on - strand at 518.200 kb on - strand at 518.202 kb on + strand at 518.202 kb on + strand at 518.202 kb on + strand at 518.202 kb on + strand at 518.208 kb on + strand at 518.218 kb on + strand at 518.218 kb on + strand at 518.223 kb on + strand at 518.312 kb on + strand at 518.322 kb on - strand at 518.391 kb on + strand at 518.402 kb on + strand at 518.410 kb on - strand at 518.410 kb on - strand at 518.412 kb on + strand at 518.412 kb on + strand at 518.414 kb on + strand at 518.424 kb on + strand at 518.434 kb on + strand at 518.445 kb on + strand at 518.473 kb on - strand, within BBR_RS12330 at 518.513 kb on + strand, within BBR_RS12330 at 518.513 kb on + strand, within BBR_RS12330 at 518.538 kb on - strand, within BBR_RS12330 at 518.538 kb on - strand, within BBR_RS12330 at 518.552 kb on + strand, within BBR_RS12330 at 518.561 kb on - strand, within BBR_RS12330 at 518.563 kb on + strand, within BBR_RS12330 at 518.577 kb on + strand, within BBR_RS12330 at 518.584 kb on + strand, within BBR_RS12330 at 518.584 kb on - strand, within BBR_RS12330 at 518.688 kb on + strand, within BBR_RS12330 at 518.688 kb on + strand, within BBR_RS12330 at 518.688 kb on + strand, within BBR_RS12330 at 518.696 kb on - strand, within BBR_RS12330 at 518.706 kb on - strand, within BBR_RS12330 at 518.708 kb on - strand, within BBR_RS12330 at 518.711 kb on - strand, within BBR_RS12330 at 518.720 kb on - strand, within BBR_RS12330 at 518.725 kb on + strand, within BBR_RS12330 at 518.726 kb on + strand, within BBR_RS12330 at 518.740 kb on + strand, within BBR_RS12330 at 518.740 kb on + strand, within BBR_RS12330 at 518.747 kb on + strand, within BBR_RS12330 at 518.747 kb on + strand, within BBR_RS12330 at 518.747 kb on + strand, within BBR_RS12330 at 518.747 kb on - strand, within BBR_RS12330 at 518.748 kb on - strand, within BBR_RS12330 at 518.755 kb on - strand, within BBR_RS12330 at 518.755 kb on - strand, within BBR_RS12330 at 518.771 kb on + strand, within BBR_RS12330 at 518.771 kb on + strand, within BBR_RS12330 at 518.779 kb on - strand, within BBR_RS12330 at 518.808 kb on + strand, within BBR_RS12330 at 518.819 kb on + strand, within BBR_RS12330 at 518.819 kb on + strand, within BBR_RS12330 at 518.827 kb on - strand, within BBR_RS12330 at 518.870 kb on - strand, within BBR_RS12330 at 518.925 kb on + strand, within BBR_RS12330 at 518.925 kb on + strand, within BBR_RS12330 at 518.927 kb on - strand, within BBR_RS12330 at 518.945 kb on + strand, within BBR_RS12330 at 518.945 kb on + strand, within BBR_RS12330
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM remove 517,070 - +0.2 517,081 - -2.3 517,086 + +1.8 517,094 - -0.8 517,094 - -0.8 517,094 - +0.1 517,094 - +0.2 517,124 + -1.4 517,134 - +0.9 517,142 - +1.2 517,142 - -0.5 517,142 - +3.5 517,142 - +0.8 517,156 + -1.6 517,165 - +1.2 517,234 - +3.2 517,255 + -0.2 517,265 - +1.4 517,275 + +1.6 517,300 + -2.2 517,300 + -2.9 517,399 + BBR_RS12325 0.18 -1.7 517,415 - BBR_RS12325 0.20 +3.8 517,429 + BBR_RS12325 0.22 +0.0 517,429 + BBR_RS12325 0.22 -1.5 517,437 - BBR_RS12325 0.23 +2.8 517,475 + BBR_RS12325 0.28 -1.2 517,508 + BBR_RS12325 0.33 +2.3 517,508 + BBR_RS12325 0.33 +3.2 517,585 + BBR_RS12325 0.43 -1.1 517,585 + BBR_RS12325 0.43 -0.1 517,585 + BBR_RS12325 0.43 +2.1 517,642 - BBR_RS12325 0.51 -1.0 517,646 - BBR_RS12325 0.52 +2.9 517,708 + BBR_RS12325 0.60 -0.2 517,712 - BBR_RS12325 0.61 +0.6 517,758 - BBR_RS12325 0.67 -1.2 517,758 - BBR_RS12325 0.67 -1.1 517,768 + BBR_RS12325 0.69 -0.4 517,770 + BBR_RS12325 0.69 +1.4 517,802 - BBR_RS12325 0.73 +0.2 517,804 + BBR_RS12325 0.74 -0.8 517,804 + BBR_RS12325 0.74 +2.1 517,804 + BBR_RS12325 0.74 -1.2 517,863 - BBR_RS12325 0.82 +1.9 517,863 - BBR_RS12325 0.82 -1.8 517,873 + BBR_RS12325 0.83 +1.1 517,974 + -0.4 517,987 + -2.1 518,052 + +3.3 518,131 - +1.9 518,147 - +3.6 518,165 + +1.3 518,185 + +1.1 518,200 - +4.0 518,200 - +2.6 518,202 + +0.9 518,202 + -0.9 518,202 + +1.8 518,202 + -0.5 518,208 + +1.9 518,218 + -0.9 518,218 + +2.7 518,223 + +0.7 518,312 + -1.1 518,322 - +1.9 518,391 + +1.0 518,402 + -1.2 518,410 - -1.5 518,410 - +1.9 518,412 + -0.7 518,412 + -0.6 518,414 + +2.5 518,424 + +3.7 518,434 + +2.0 518,445 + +1.6 518,473 - BBR_RS12330 0.11 +2.6 518,513 + BBR_RS12330 0.13 -0.6 518,513 + BBR_RS12330 0.13 +2.0 518,538 - BBR_RS12330 0.14 +2.7 518,538 - BBR_RS12330 0.14 -1.6 518,552 + BBR_RS12330 0.14 +2.1 518,561 - BBR_RS12330 0.15 +2.5 518,563 + BBR_RS12330 0.15 -0.8 518,577 + BBR_RS12330 0.16 +1.1 518,584 + BBR_RS12330 0.16 +1.3 518,584 - BBR_RS12330 0.16 -0.1 518,688 + BBR_RS12330 0.21 +2.0 518,688 + BBR_RS12330 0.21 -0.8 518,688 + BBR_RS12330 0.21 -1.4 518,696 - BBR_RS12330 0.21 -1.2 518,706 - BBR_RS12330 0.21 +3.7 518,708 - BBR_RS12330 0.22 +2.2 518,711 - BBR_RS12330 0.22 +2.1 518,720 - BBR_RS12330 0.22 -1.5 518,725 + BBR_RS12330 0.22 -0.5 518,726 + BBR_RS12330 0.22 +4.8 518,740 + BBR_RS12330 0.23 +1.1 518,740 + BBR_RS12330 0.23 +0.5 518,747 + BBR_RS12330 0.23 -1.0 518,747 + BBR_RS12330 0.23 +3.5 518,747 + BBR_RS12330 0.23 +1.3 518,747 - BBR_RS12330 0.23 +0.7 518,748 - BBR_RS12330 0.23 +2.3 518,755 - BBR_RS12330 0.24 +0.4 518,755 - BBR_RS12330 0.24 +2.6 518,771 + BBR_RS12330 0.24 +1.3 518,771 + BBR_RS12330 0.24 +1.1 518,779 - BBR_RS12330 0.25 -2.0 518,808 + BBR_RS12330 0.26 -0.9 518,819 + BBR_RS12330 0.27 +1.7 518,819 + BBR_RS12330 0.27 -1.8 518,827 - BBR_RS12330 0.27 +0.2 518,870 - BBR_RS12330 0.29 +0.1 518,925 + BBR_RS12330 0.31 -0.2 518,925 + BBR_RS12330 0.31 +4.0 518,927 - BBR_RS12330 0.31 -1.1 518,945 + BBR_RS12330 0.32 -1.7 518,945 + BBR_RS12330 0.32 +3.7
Or see this region's nucleotide sequence