Experiment: reconstituted MRS with Difucosyllactose 7.9 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12315 and BBR_RS12320 are separated by 248 nucleotides BBR_RS12320 and BBR_RS12325 are separated by 210 nucleotides
BBR_RS12315: BBR_RS12315 - cation transporter, at 514,251 to 515,159
_RS12315
BBR_RS12320: BBR_RS12320 - cysteine--tRNA ligase, at 515,408 to 517,057
_RS12320
BBR_RS12325: BBR_RS12325 - type 1 glutamine amidotransferase, at 517,268 to 517,996
_RS12325
Position (kb) 515
516
517
518 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 514.437 kb on + strand, within BBR_RS12315 at 514.439 kb on + strand, within BBR_RS12315 at 514.439 kb on + strand, within BBR_RS12315 at 514.441 kb on + strand, within BBR_RS12315 at 514.445 kb on - strand, within BBR_RS12315 at 514.445 kb on - strand, within BBR_RS12315 at 514.445 kb on - strand, within BBR_RS12315 at 514.445 kb on - strand, within BBR_RS12315 at 514.445 kb on - strand, within BBR_RS12315 at 514.452 kb on - strand, within BBR_RS12315 at 514.469 kb on + strand, within BBR_RS12315 at 514.482 kb on - strand, within BBR_RS12315 at 514.482 kb on - strand, within BBR_RS12315 at 514.496 kb on + strand, within BBR_RS12315 at 514.511 kb on - strand, within BBR_RS12315 at 514.534 kb on - strand, within BBR_RS12315 at 514.550 kb on - strand, within BBR_RS12315 at 514.557 kb on + strand, within BBR_RS12315 at 514.572 kb on + strand, within BBR_RS12315 at 514.592 kb on + strand, within BBR_RS12315 at 514.592 kb on + strand, within BBR_RS12315 at 514.600 kb on - strand, within BBR_RS12315 at 514.631 kb on - strand, within BBR_RS12315 at 514.642 kb on + strand, within BBR_RS12315 at 514.648 kb on + strand, within BBR_RS12315 at 514.650 kb on - strand, within BBR_RS12315 at 514.650 kb on - strand, within BBR_RS12315 at 514.650 kb on - strand, within BBR_RS12315 at 514.656 kb on - strand, within BBR_RS12315 at 514.691 kb on + strand, within BBR_RS12315 at 514.691 kb on + strand, within BBR_RS12315 at 514.691 kb on + strand, within BBR_RS12315 at 514.691 kb on + strand, within BBR_RS12315 at 514.691 kb on + strand, within BBR_RS12315 at 514.691 kb on + strand, within BBR_RS12315 at 514.699 kb on - strand, within BBR_RS12315 at 514.711 kb on - strand, within BBR_RS12315 at 514.758 kb on + strand, within BBR_RS12315 at 514.761 kb on - strand, within BBR_RS12315 at 514.768 kb on + strand, within BBR_RS12315 at 514.800 kb on + strand, within BBR_RS12315 at 514.822 kb on - strand, within BBR_RS12315 at 514.822 kb on - strand, within BBR_RS12315 at 514.827 kb on - strand, within BBR_RS12315 at 514.827 kb on - strand, within BBR_RS12315 at 514.847 kb on - strand, within BBR_RS12315 at 514.852 kb on - strand, within BBR_RS12315 at 514.864 kb on + strand, within BBR_RS12315 at 514.864 kb on + strand, within BBR_RS12315 at 514.872 kb on - strand, within BBR_RS12315 at 514.946 kb on + strand, within BBR_RS12315 at 514.975 kb on + strand, within BBR_RS12315 at 515.021 kb on + strand, within BBR_RS12315 at 515.021 kb on + strand, within BBR_RS12315 at 515.024 kb on - strand, within BBR_RS12315 at 515.026 kb on + strand, within BBR_RS12315 at 515.032 kb on - strand, within BBR_RS12315 at 515.039 kb on - strand, within BBR_RS12315 at 515.058 kb on - strand, within BBR_RS12315 at 515.058 kb on - strand, within BBR_RS12315 at 515.109 kb on + strand at 515.121 kb on + strand at 515.137 kb on - strand at 515.144 kb on + strand at 515.144 kb on + strand at 515.186 kb on + strand at 515.225 kb on - strand at 515.244 kb on - strand at 515.291 kb on + strand at 515.291 kb on + strand at 515.301 kb on - strand at 515.301 kb on - strand at 515.307 kb on + strand at 515.313 kb on - strand at 515.317 kb on + strand at 515.355 kb on + strand at 515.368 kb on - strand at 517.070 kb on - strand at 517.081 kb on - strand at 517.086 kb on + strand at 517.094 kb on - strand at 517.094 kb on - strand at 517.094 kb on - strand at 517.094 kb on - strand at 517.124 kb on + strand at 517.134 kb on - strand at 517.142 kb on - strand at 517.142 kb on - strand at 517.142 kb on - strand at 517.142 kb on - strand at 517.156 kb on + strand at 517.165 kb on - strand at 517.234 kb on - strand at 517.255 kb on + strand at 517.265 kb on - strand at 517.275 kb on + strand at 517.300 kb on + strand at 517.300 kb on + strand at 517.399 kb on + strand, within BBR_RS12325 at 517.415 kb on - strand, within BBR_RS12325 at 517.429 kb on + strand, within BBR_RS12325 at 517.429 kb on + strand, within BBR_RS12325 at 517.437 kb on - strand, within BBR_RS12325 at 517.475 kb on + strand, within BBR_RS12325 at 517.508 kb on + strand, within BBR_RS12325 at 517.508 kb on + strand, within BBR_RS12325 at 517.585 kb on + strand, within BBR_RS12325 at 517.585 kb on + strand, within BBR_RS12325 at 517.585 kb on + strand, within BBR_RS12325 at 517.642 kb on - strand, within BBR_RS12325 at 517.646 kb on - strand, within BBR_RS12325 at 517.708 kb on + strand, within BBR_RS12325 at 517.712 kb on - strand, within BBR_RS12325 at 517.758 kb on - strand, within BBR_RS12325 at 517.758 kb on - strand, within BBR_RS12325 at 517.768 kb on + strand, within BBR_RS12325 at 517.770 kb on + strand, within BBR_RS12325 at 517.802 kb on - strand, within BBR_RS12325 at 517.804 kb on + strand, within BBR_RS12325 at 517.804 kb on + strand, within BBR_RS12325 at 517.804 kb on + strand, within BBR_RS12325 at 517.863 kb on - strand, within BBR_RS12325 at 517.863 kb on - strand, within BBR_RS12325 at 517.873 kb on + strand, within BBR_RS12325 at 517.974 kb on + strand at 517.987 kb on + strand at 518.052 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Difucosyllactose 7.9 mM remove 514,437 + BBR_RS12315 0.20 -2.6 514,439 + BBR_RS12315 0.21 +3.5 514,439 + BBR_RS12315 0.21 -0.4 514,441 + BBR_RS12315 0.21 -0.2 514,445 - BBR_RS12315 0.21 -0.7 514,445 - BBR_RS12315 0.21 +3.2 514,445 - BBR_RS12315 0.21 -1.3 514,445 - BBR_RS12315 0.21 -0.6 514,445 - BBR_RS12315 0.21 -1.1 514,452 - BBR_RS12315 0.22 -0.8 514,469 + BBR_RS12315 0.24 -0.8 514,482 - BBR_RS12315 0.25 -0.7 514,482 - BBR_RS12315 0.25 -1.0 514,496 + BBR_RS12315 0.27 -0.5 514,511 - BBR_RS12315 0.29 +1.7 514,534 - BBR_RS12315 0.31 +1.7 514,550 - BBR_RS12315 0.33 -0.2 514,557 + BBR_RS12315 0.34 +1.8 514,572 + BBR_RS12315 0.35 +0.9 514,592 + BBR_RS12315 0.38 -2.0 514,592 + BBR_RS12315 0.38 +2.4 514,600 - BBR_RS12315 0.38 -0.2 514,631 - BBR_RS12315 0.42 -1.1 514,642 + BBR_RS12315 0.43 +2.4 514,648 + BBR_RS12315 0.44 +1.3 514,650 - BBR_RS12315 0.44 -0.5 514,650 - BBR_RS12315 0.44 +2.1 514,650 - BBR_RS12315 0.44 -1.1 514,656 - BBR_RS12315 0.45 -1.0 514,691 + BBR_RS12315 0.48 -1.1 514,691 + BBR_RS12315 0.48 +0.3 514,691 + BBR_RS12315 0.48 +2.7 514,691 + BBR_RS12315 0.48 -1.1 514,691 + BBR_RS12315 0.48 +0.4 514,691 + BBR_RS12315 0.48 +0.5 514,699 - BBR_RS12315 0.49 -0.1 514,711 - BBR_RS12315 0.51 -0.3 514,758 + BBR_RS12315 0.56 -0.9 514,761 - BBR_RS12315 0.56 -0.2 514,768 + BBR_RS12315 0.57 +0.2 514,800 + BBR_RS12315 0.60 +2.9 514,822 - BBR_RS12315 0.63 +1.6 514,822 - BBR_RS12315 0.63 -1.3 514,827 - BBR_RS12315 0.63 +0.7 514,827 - BBR_RS12315 0.63 -0.1 514,847 - BBR_RS12315 0.66 +0.3 514,852 - BBR_RS12315 0.66 +0.8 514,864 + BBR_RS12315 0.67 -1.2 514,864 + BBR_RS12315 0.67 -1.1 514,872 - BBR_RS12315 0.68 +0.1 514,946 + BBR_RS12315 0.76 -0.1 514,975 + BBR_RS12315 0.80 -0.9 515,021 + BBR_RS12315 0.85 -0.7 515,021 + BBR_RS12315 0.85 -0.4 515,024 - BBR_RS12315 0.85 +1.7 515,026 + BBR_RS12315 0.85 +2.9 515,032 - BBR_RS12315 0.86 -0.8 515,039 - BBR_RS12315 0.87 -0.9 515,058 - BBR_RS12315 0.89 -0.9 515,058 - BBR_RS12315 0.89 +2.6 515,109 + +1.6 515,121 + +1.9 515,137 - -0.9 515,144 + +0.0 515,144 + -1.4 515,186 + -2.1 515,225 - -0.4 515,244 - -1.2 515,291 + +2.6 515,291 + +2.0 515,301 - -1.4 515,301 - -1.0 515,307 + -1.8 515,313 - -0.6 515,317 + -0.6 515,355 + -1.8 515,368 - -0.4 517,070 - +0.2 517,081 - -2.3 517,086 + +1.8 517,094 - -0.8 517,094 - -0.8 517,094 - +0.1 517,094 - +0.2 517,124 + -1.4 517,134 - +0.9 517,142 - +1.2 517,142 - -0.5 517,142 - +3.5 517,142 - +0.8 517,156 + -1.6 517,165 - +1.2 517,234 - +3.2 517,255 + -0.2 517,265 - +1.4 517,275 + +1.6 517,300 + -2.2 517,300 + -2.9 517,399 + BBR_RS12325 0.18 -1.7 517,415 - BBR_RS12325 0.20 +3.8 517,429 + BBR_RS12325 0.22 +0.0 517,429 + BBR_RS12325 0.22 -1.5 517,437 - BBR_RS12325 0.23 +2.8 517,475 + BBR_RS12325 0.28 -1.2 517,508 + BBR_RS12325 0.33 +2.3 517,508 + BBR_RS12325 0.33 +3.2 517,585 + BBR_RS12325 0.43 -1.1 517,585 + BBR_RS12325 0.43 -0.1 517,585 + BBR_RS12325 0.43 +2.1 517,642 - BBR_RS12325 0.51 -1.0 517,646 - BBR_RS12325 0.52 +2.9 517,708 + BBR_RS12325 0.60 -0.2 517,712 - BBR_RS12325 0.61 +0.6 517,758 - BBR_RS12325 0.67 -1.2 517,758 - BBR_RS12325 0.67 -1.1 517,768 + BBR_RS12325 0.69 -0.4 517,770 + BBR_RS12325 0.69 +1.4 517,802 - BBR_RS12325 0.73 +0.2 517,804 + BBR_RS12325 0.74 -0.8 517,804 + BBR_RS12325 0.74 +2.1 517,804 + BBR_RS12325 0.74 -1.2 517,863 - BBR_RS12325 0.82 +1.9 517,863 - BBR_RS12325 0.82 -1.8 517,873 + BBR_RS12325 0.83 +1.1 517,974 + -0.4 517,987 + -2.1 518,052 + +3.3
Or see this region's nucleotide sequence