Experiment: m.b. Cisplatin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p01760 and PGA1_262p01770 are separated by 62 nucleotides PGA1_262p01770 and PGA1_262p01780 are separated by 121 nucleotides PGA1_262p01780 and PGA1_262p01790 are separated by 91 nucleotides
PGA1_262p01760: PGA1_262p01760 - epoxide hydrolase, similar to eukaryotic, at 193,416 to 194,588
_262p01760
PGA1_262p01770: PGA1_262p01770 - putative endoribonuclease, at 194,651 to 194,992
_262p01770
PGA1_262p01780: PGA1_262p01780 - transcriptional regulator, LysR family, at 195,114 to 196,019
_262p01780
PGA1_262p01790: PGA1_262p01790 - aldehyde dehydrogenase, at 196,111 to 197,562
_262p01790
Position (kb) 195
196
197 Strain fitness (log2 ratio) -2
-1
0
1
2 at 194.164 kb on + strand, within PGA1_262p01760 at 194.172 kb on - strand, within PGA1_262p01760 at 194.302 kb on + strand, within PGA1_262p01760 at 194.321 kb on + strand, within PGA1_262p01760 at 194.403 kb on + strand, within PGA1_262p01760 at 194.414 kb on - strand, within PGA1_262p01760 at 194.446 kb on - strand, within PGA1_262p01760 at 194.456 kb on + strand, within PGA1_262p01760 at 194.456 kb on + strand, within PGA1_262p01760 at 194.456 kb on + strand, within PGA1_262p01760 at 194.459 kb on - strand, within PGA1_262p01760 at 194.464 kb on - strand, within PGA1_262p01760 at 194.464 kb on - strand, within PGA1_262p01760 at 194.464 kb on - strand, within PGA1_262p01760 at 194.486 kb on + strand at 194.615 kb on + strand at 194.623 kb on - strand at 194.712 kb on - strand, within PGA1_262p01770 at 194.835 kb on + strand, within PGA1_262p01770 at 194.846 kb on + strand, within PGA1_262p01770 at 194.854 kb on - strand, within PGA1_262p01770 at 194.854 kb on - strand, within PGA1_262p01770 at 194.865 kb on - strand, within PGA1_262p01770 at 194.876 kb on + strand, within PGA1_262p01770 at 194.876 kb on + strand, within PGA1_262p01770 at 194.876 kb on + strand, within PGA1_262p01770 at 194.906 kb on - strand, within PGA1_262p01770 at 194.912 kb on + strand, within PGA1_262p01770 at 194.937 kb on + strand, within PGA1_262p01770 at 194.942 kb on - strand, within PGA1_262p01770 at 194.973 kb on + strand at 195.026 kb on - strand at 195.038 kb on + strand at 195.046 kb on + strand at 195.124 kb on + strand at 195.132 kb on - strand at 195.132 kb on - strand at 195.134 kb on + strand at 195.134 kb on + strand at 195.159 kb on + strand at 195.173 kb on + strand at 195.173 kb on + strand at 195.181 kb on - strand at 195.181 kb on - strand at 195.181 kb on - strand at 195.260 kb on + strand, within PGA1_262p01780 at 195.268 kb on - strand, within PGA1_262p01780 at 195.527 kb on - strand, within PGA1_262p01780 at 195.588 kb on + strand, within PGA1_262p01780 at 195.808 kb on + strand, within PGA1_262p01780 at 195.808 kb on + strand, within PGA1_262p01780 at 196.061 kb on + strand at 196.072 kb on + strand at 196.072 kb on + strand at 196.074 kb on + strand at 196.080 kb on - strand at 196.151 kb on - strand at 196.192 kb on - strand at 196.192 kb on - strand at 196.226 kb on + strand at 196.255 kb on - strand at 196.280 kb on - strand, within PGA1_262p01790 at 196.280 kb on - strand, within PGA1_262p01790 at 196.354 kb on - strand, within PGA1_262p01790 at 196.383 kb on + strand, within PGA1_262p01790 at 196.421 kb on + strand, within PGA1_262p01790 at 196.431 kb on + strand, within PGA1_262p01790 at 196.439 kb on - strand, within PGA1_262p01790 at 196.478 kb on - strand, within PGA1_262p01790 at 196.540 kb on + strand, within PGA1_262p01790 at 196.602 kb on + strand, within PGA1_262p01790 at 196.757 kb on + strand, within PGA1_262p01790 at 196.842 kb on + strand, within PGA1_262p01790 at 196.895 kb on + strand, within PGA1_262p01790
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.0125 mg/ml remove 194,164 + PGA1_262p01760 0.64 +0.0 194,172 - PGA1_262p01760 0.64 -0.4 194,302 + PGA1_262p01760 0.76 -0.3 194,321 + PGA1_262p01760 0.77 -0.2 194,403 + PGA1_262p01760 0.84 -0.1 194,414 - PGA1_262p01760 0.85 -0.2 194,446 - PGA1_262p01760 0.88 -1.0 194,456 + PGA1_262p01760 0.89 -0.2 194,456 + PGA1_262p01760 0.89 +0.6 194,456 + PGA1_262p01760 0.89 -0.5 194,459 - PGA1_262p01760 0.89 +1.4 194,464 - PGA1_262p01760 0.89 -2.4 194,464 - PGA1_262p01760 0.89 -0.2 194,464 - PGA1_262p01760 0.89 -0.5 194,486 + +0.7 194,615 + +0.6 194,623 - +0.0 194,712 - PGA1_262p01770 0.18 -1.0 194,835 + PGA1_262p01770 0.54 +0.0 194,846 + PGA1_262p01770 0.57 -1.4 194,854 - PGA1_262p01770 0.59 -0.8 194,854 - PGA1_262p01770 0.59 +1.0 194,865 - PGA1_262p01770 0.63 +0.3 194,876 + PGA1_262p01770 0.66 +1.4 194,876 + PGA1_262p01770 0.66 -0.9 194,876 + PGA1_262p01770 0.66 -1.0 194,906 - PGA1_262p01770 0.75 -0.4 194,912 + PGA1_262p01770 0.76 +0.4 194,937 + PGA1_262p01770 0.84 +0.5 194,942 - PGA1_262p01770 0.85 -0.6 194,973 + -0.8 195,026 - -0.5 195,038 + -0.9 195,046 + +0.7 195,124 + -1.4 195,132 - +0.4 195,132 - +1.3 195,134 + -1.5 195,134 + -0.7 195,159 + +0.7 195,173 + -0.2 195,173 + -0.2 195,181 - -0.5 195,181 - +1.5 195,181 - -0.5 195,260 + PGA1_262p01780 0.16 +0.3 195,268 - PGA1_262p01780 0.17 +0.0 195,527 - PGA1_262p01780 0.46 +1.3 195,588 + PGA1_262p01780 0.52 +0.4 195,808 + PGA1_262p01780 0.77 +2.3 195,808 + PGA1_262p01780 0.77 +0.4 196,061 + -0.6 196,072 + -2.0 196,072 + +1.8 196,074 + -1.0 196,080 - -2.0 196,151 - -1.8 196,192 - -0.2 196,192 - +0.9 196,226 + +0.8 196,255 - +0.2 196,280 - PGA1_262p01790 0.12 +1.8 196,280 - PGA1_262p01790 0.12 -1.7 196,354 - PGA1_262p01790 0.17 -0.8 196,383 + PGA1_262p01790 0.19 -0.5 196,421 + PGA1_262p01790 0.21 +1.5 196,431 + PGA1_262p01790 0.22 -1.3 196,439 - PGA1_262p01790 0.23 +0.0 196,478 - PGA1_262p01790 0.25 +0.4 196,540 + PGA1_262p01790 0.30 +0.5 196,602 + PGA1_262p01790 0.34 +1.8 196,757 + PGA1_262p01790 0.44 -2.1 196,842 + PGA1_262p01790 0.50 +0.6 196,895 + PGA1_262p01790 0.54 +0.3
Or see this region's nucleotide sequence