Experiment: L-Malic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_293 and HP15_294 are separated by 210 nucleotides HP15_294 and HP15_295 are separated by 7 nucleotides HP15_295 and HP15_296 are separated by 267 nucleotides
HP15_293: HP15_293 - DNA polymerase I, at 284,328 to 286,936
_293
HP15_294: HP15_294 - conserved hypothetical protein, secreted, at 287,147 to 287,485
_294
HP15_295: HP15_295 - fibronectin, type III domain protein, at 287,493 to 287,984
_295
HP15_296: HP15_296 - conserved hypothetical protein, at 288,252 to 288,674
_296
Position (kb) 287
288 Strain fitness (log2 ratio) -1
0
1
2 at 287.059 kb on + strand at 287.092 kb on + strand at 287.102 kb on + strand at 287.105 kb on - strand at 287.323 kb on - strand, within HP15_294 at 287.339 kb on + strand, within HP15_294 at 287.395 kb on + strand, within HP15_294 at 287.504 kb on - strand at 287.643 kb on - strand, within HP15_295 at 287.721 kb on + strand, within HP15_295 at 287.729 kb on + strand, within HP15_295 at 287.754 kb on - strand, within HP15_295 at 287.852 kb on + strand, within HP15_295 at 288.205 kb on + strand at 288.218 kb on - strand at 288.260 kb on + strand at 288.268 kb on - strand at 288.348 kb on - strand, within HP15_296 at 288.384 kb on + strand, within HP15_296 at 288.388 kb on + strand, within HP15_296
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic remove 287,059 + +1.8 287,092 + -0.2 287,102 + +0.4 287,105 - -0.7 287,323 - HP15_294 0.52 -0.1 287,339 + HP15_294 0.57 +1.2 287,395 + HP15_294 0.73 +0.9 287,504 - -0.0 287,643 - HP15_295 0.30 -0.7 287,721 + HP15_295 0.46 +0.7 287,729 + HP15_295 0.48 +0.6 287,754 - HP15_295 0.53 -0.1 287,852 + HP15_295 0.73 -0.1 288,205 + +0.8 288,218 - +1.6 288,260 + -0.1 288,268 - -0.3 288,348 - HP15_296 0.23 -0.2 288,384 + HP15_296 0.31 +0.0 288,388 + HP15_296 0.32 +0.2
Or see this region's nucleotide sequence