Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27715

Experiment: D-Alanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27710 and BWI76_RS27715 are separated by 24 nucleotidesBWI76_RS27715 and BWI76_RS27720 are separated by 249 nucleotides BWI76_RS27710: BWI76_RS27710 - N-acetylmuramic acid 6-phosphate etherase, at 5,751,207 to 5,752,109 _RS27710 BWI76_RS27715: BWI76_RS27715 - PTS N-acetylmuramic acid transporter subunits IIBC, at 5,752,134 to 5,753,567 _RS27715 BWI76_RS27720: BWI76_RS27720 - carboxylate/amino acid/amine transporter, at 5,753,817 to 5,754,719 _RS27720 Position (kb) 5752 5753 5754Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5751.332 kb on - strand, within BWI76_RS27710at 5751.345 kb on - strand, within BWI76_RS27710at 5751.574 kb on + strand, within BWI76_RS27710at 5751.612 kb on + strand, within BWI76_RS27710at 5751.666 kb on + strand, within BWI76_RS27710at 5751.800 kb on + strand, within BWI76_RS27710at 5751.802 kb on - strand, within BWI76_RS27710at 5751.856 kb on + strand, within BWI76_RS27710at 5751.866 kb on + strand, within BWI76_RS27710at 5751.867 kb on + strand, within BWI76_RS27710at 5751.867 kb on + strand, within BWI76_RS27710at 5751.867 kb on + strand, within BWI76_RS27710at 5751.874 kb on - strand, within BWI76_RS27710at 5751.875 kb on - strand, within BWI76_RS27710at 5752.092 kb on + strandat 5752.100 kb on - strandat 5752.102 kb on + strandat 5752.105 kb on - strandat 5752.288 kb on - strand, within BWI76_RS27715at 5752.327 kb on - strand, within BWI76_RS27715at 5752.549 kb on - strand, within BWI76_RS27715at 5752.632 kb on - strand, within BWI76_RS27715at 5752.692 kb on + strand, within BWI76_RS27715at 5752.736 kb on + strand, within BWI76_RS27715at 5753.007 kb on - strand, within BWI76_RS27715at 5753.018 kb on + strand, within BWI76_RS27715at 5753.046 kb on - strand, within BWI76_RS27715at 5753.096 kb on - strand, within BWI76_RS27715at 5753.110 kb on + strand, within BWI76_RS27715at 5753.120 kb on + strand, within BWI76_RS27715at 5753.142 kb on - strand, within BWI76_RS27715at 5753.180 kb on + strand, within BWI76_RS27715at 5753.193 kb on - strand, within BWI76_RS27715at 5753.531 kb on - strandat 5753.531 kb on - strandat 5753.681 kb on + strandat 5753.716 kb on + strandat 5753.724 kb on - strandat 5753.724 kb on - strandat 5753.798 kb on + strandat 5753.857 kb on + strandat 5753.883 kb on - strandat 5753.943 kb on + strand, within BWI76_RS27720at 5753.992 kb on + strand, within BWI76_RS27720at 5754.116 kb on + strand, within BWI76_RS27720at 5754.196 kb on - strand, within BWI76_RS27720at 5754.288 kb on + strand, within BWI76_RS27720at 5754.293 kb on + strand, within BWI76_RS27720at 5754.293 kb on + strand, within BWI76_RS27720at 5754.296 kb on - strand, within BWI76_RS27720at 5754.301 kb on - strand, within BWI76_RS27720at 5754.301 kb on - strand, within BWI76_RS27720at 5754.457 kb on + strand, within BWI76_RS27720at 5754.502 kb on - strand, within BWI76_RS27720at 5754.511 kb on + strand, within BWI76_RS27720at 5754.519 kb on - strand, within BWI76_RS27720at 5754.519 kb on - strand, within BWI76_RS27720

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Alanine (N)
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5,751,332 - BWI76_RS27710 0.14 -1.1
5,751,345 - BWI76_RS27710 0.15 -3.5
5,751,574 + BWI76_RS27710 0.41 +0.0
5,751,612 + BWI76_RS27710 0.45 -0.2
5,751,666 + BWI76_RS27710 0.51 +0.8
5,751,800 + BWI76_RS27710 0.66 +0.0
5,751,802 - BWI76_RS27710 0.66 +0.6
5,751,856 + BWI76_RS27710 0.72 +0.8
5,751,866 + BWI76_RS27710 0.73 -1.0
5,751,867 + BWI76_RS27710 0.73 +1.9
5,751,867 + BWI76_RS27710 0.73 +0.4
5,751,867 + BWI76_RS27710 0.73 +0.4
5,751,874 - BWI76_RS27710 0.74 +0.4
5,751,875 - BWI76_RS27710 0.74 -0.1
5,752,092 + -0.6
5,752,100 - +0.5
5,752,102 + -0.6
5,752,105 - +1.1
5,752,288 - BWI76_RS27715 0.11 -1.6
5,752,327 - BWI76_RS27715 0.13 -0.1
5,752,549 - BWI76_RS27715 0.29 -0.6
5,752,632 - BWI76_RS27715 0.35 -0.7
5,752,692 + BWI76_RS27715 0.39 +0.2
5,752,736 + BWI76_RS27715 0.42 +0.4
5,753,007 - BWI76_RS27715 0.61 +0.2
5,753,018 + BWI76_RS27715 0.62 -0.2
5,753,046 - BWI76_RS27715 0.64 -2.0
5,753,096 - BWI76_RS27715 0.67 -1.6
5,753,110 + BWI76_RS27715 0.68 -0.3
5,753,120 + BWI76_RS27715 0.69 -0.9
5,753,142 - BWI76_RS27715 0.70 +2.6
5,753,180 + BWI76_RS27715 0.73 +0.0
5,753,193 - BWI76_RS27715 0.74 -0.7
5,753,531 - +1.8
5,753,531 - -0.0
5,753,681 + +1.4
5,753,716 + -0.8
5,753,724 - -2.0
5,753,724 - +0.3
5,753,798 + -2.1
5,753,857 + +0.7
5,753,883 - +0.7
5,753,943 + BWI76_RS27720 0.14 -0.5
5,753,992 + BWI76_RS27720 0.19 -0.7
5,754,116 + BWI76_RS27720 0.33 -1.8
5,754,196 - BWI76_RS27720 0.42 +1.3
5,754,288 + BWI76_RS27720 0.52 +0.6
5,754,293 + BWI76_RS27720 0.53 -1.3
5,754,293 + BWI76_RS27720 0.53 -3.3
5,754,296 - BWI76_RS27720 0.53 +0.6
5,754,301 - BWI76_RS27720 0.54 +0.7
5,754,301 - BWI76_RS27720 0.54 -1.0
5,754,457 + BWI76_RS27720 0.71 +0.3
5,754,502 - BWI76_RS27720 0.76 +0.0
5,754,511 + BWI76_RS27720 0.77 +0.6
5,754,519 - BWI76_RS27720 0.78 +0.3
5,754,519 - BWI76_RS27720 0.78 +0.6

Or see this region's nucleotide sequence