Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15975

Experiment: D-Alanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15970 and BWI76_RS15975 are separated by 176 nucleotidesBWI76_RS15975 and BWI76_RS15980 are separated by 149 nucleotidesBWI76_RS15980 and BWI76_RS15985 overlap by 20 nucleotides BWI76_RS15970: BWI76_RS15970 - carboxypeptidase M32, at 3,313,078 to 3,314,565 _RS15970 BWI76_RS15975: BWI76_RS15975 - hypothetical protein, at 3,314,742 to 3,315,167 _RS15975 BWI76_RS15980: BWI76_RS15980 - lysine transporter LysE, at 3,315,317 to 3,315,579 _RS15980 BWI76_RS15985: BWI76_RS15985 - LysR family transcriptional regulator, at 3,315,560 to 3,315,784 _RS15985 Position (kb) 3314 3315 3316Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3314.303 kb on - strand, within BWI76_RS15970at 3314.645 kb on + strandat 3314.730 kb on + strandat 3314.782 kb on - strandat 3314.982 kb on + strand, within BWI76_RS15975at 3315.261 kb on - strandat 3315.433 kb on - strand, within BWI76_RS15980at 3315.584 kb on + strand, within BWI76_RS15985at 3315.602 kb on + strand, within BWI76_RS15985at 3315.610 kb on - strand, within BWI76_RS15985at 3315.746 kb on - strand, within BWI76_RS15985at 3315.951 kb on + strandat 3315.968 kb on - strandat 3316.079 kb on - strandat 3316.079 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Alanine (N)
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3,314,303 - BWI76_RS15970 0.82 +1.2
3,314,645 + +1.1
3,314,730 + -0.5
3,314,782 - +0.6
3,314,982 + BWI76_RS15975 0.56 -3.9
3,315,261 - -1.1
3,315,433 - BWI76_RS15980 0.44 +0.3
3,315,584 + BWI76_RS15985 0.11 +0.0
3,315,602 + BWI76_RS15985 0.19 -1.1
3,315,610 - BWI76_RS15985 0.22 -0.8
3,315,746 - BWI76_RS15985 0.83 +0.3
3,315,951 + -3.5
3,315,968 - +0.9
3,316,079 - -2.5
3,316,079 - -1.3

Or see this region's nucleotide sequence