Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10035

Experiment: D-Alanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10030 and BWI76_RS10035 are separated by 42 nucleotidesBWI76_RS10035 and BWI76_RS10040 are separated by 36 nucleotidesBWI76_RS10040 and BWI76_RS10045 are separated by 60 nucleotides BWI76_RS10030: BWI76_RS10030 - glycosyl transferase, at 2,123,790 to 2,126,933 _RS10030 BWI76_RS10035: BWI76_RS10035 - hypothetical protein, at 2,126,976 to 2,127,908 _RS10035 BWI76_RS10040: BWI76_RS10040 - heat-shock protein HspQ, at 2,127,945 to 2,128,262 _RS10040 BWI76_RS10045: BWI76_RS10045 - 23S rRNA (cytosine(1962)-C(5))-methyltransferase, at 2,128,323 to 2,129,525 _RS10045 Position (kb) 2126 2127 2128Strain fitness (log2 ratio) -2 -1 0 1 2at 2126.146 kb on - strand, within BWI76_RS10030at 2126.443 kb on + strand, within BWI76_RS10030at 2126.451 kb on - strand, within BWI76_RS10030at 2126.451 kb on - strandat 2126.503 kb on - strand, within BWI76_RS10030at 2126.770 kb on + strandat 2126.859 kb on + strandat 2126.880 kb on + strandat 2126.888 kb on - strandat 2127.018 kb on - strandat 2127.267 kb on + strand, within BWI76_RS10035at 2127.326 kb on + strand, within BWI76_RS10035at 2127.835 kb on + strandat 2128.217 kb on - strand, within BWI76_RS10040at 2128.495 kb on - strand, within BWI76_RS10045at 2128.714 kb on - strand, within BWI76_RS10045at 2128.762 kb on - strand, within BWI76_RS10045at 2128.900 kb on - strand, within BWI76_RS10045

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Alanine (N)
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2,126,146 - BWI76_RS10030 0.75 +0.5
2,126,443 + BWI76_RS10030 0.84 +0.9
2,126,451 - BWI76_RS10030 0.85 +1.6
2,126,451 - +0.1
2,126,503 - BWI76_RS10030 0.86 +0.3
2,126,770 + +0.3
2,126,859 + +2.0
2,126,880 + +0.1
2,126,888 - -1.7
2,127,018 - -0.7
2,127,267 + BWI76_RS10035 0.31 -2.6
2,127,326 + BWI76_RS10035 0.38 -1.3
2,127,835 + -1.8
2,128,217 - BWI76_RS10040 0.86 -0.5
2,128,495 - BWI76_RS10045 0.14 -1.5
2,128,714 - BWI76_RS10045 0.33 -1.9
2,128,762 - BWI76_RS10045 0.36 +0.1
2,128,900 - BWI76_RS10045 0.48 -0.0

Or see this region's nucleotide sequence