Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04950

Experiment: D-Alanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04945 and BWI76_RS04950 are separated by 80 nucleotidesBWI76_RS04950 and BWI76_RS04955 are separated by 203 nucleotidesBWI76_RS04955 and BWI76_RS04960 are separated by 50 nucleotides BWI76_RS04945: BWI76_RS04945 - multicopper oxidase, at 1,037,326 to 1,038,909 _RS04945 BWI76_RS04950: BWI76_RS04950 - membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family, at 1,038,990 to 1,041,380 _RS04950 BWI76_RS04955: BWI76_RS04955 - hypoxanthine phosphoribosyltransferase, at 1,041,584 to 1,042,120 _RS04955 BWI76_RS04960: BWI76_RS04960 - carbonate dehydratase, at 1,042,171 to 1,042,833 _RS04960 Position (kb) 1038 1039 1040 1041 1042Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 1038.283 kb on + strand, within BWI76_RS04945at 1038.350 kb on + strand, within BWI76_RS04945at 1038.540 kb on + strand, within BWI76_RS04945at 1038.548 kb on - strand, within BWI76_RS04945at 1038.650 kb on - strand, within BWI76_RS04945at 1039.097 kb on + strandat 1039.258 kb on + strand, within BWI76_RS04950at 1039.310 kb on - strand, within BWI76_RS04950at 1039.373 kb on - strand, within BWI76_RS04950at 1039.639 kb on + strand, within BWI76_RS04950at 1040.046 kb on - strand, within BWI76_RS04950at 1040.075 kb on - strand, within BWI76_RS04950at 1040.286 kb on + strand, within BWI76_RS04950at 1040.464 kb on - strand, within BWI76_RS04950at 1040.694 kb on - strand, within BWI76_RS04950at 1040.852 kb on - strand, within BWI76_RS04950at 1040.873 kb on - strand, within BWI76_RS04950at 1041.073 kb on - strand, within BWI76_RS04950at 1041.210 kb on - strandat 1041.258 kb on - strandat 1041.498 kb on + strandat 1041.749 kb on + strand, within BWI76_RS04955at 1041.801 kb on - strand, within BWI76_RS04955at 1042.160 kb on - strandat 1042.160 kb on - strandat 1042.279 kb on + strand, within BWI76_RS04960at 1042.303 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Alanine (N)
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1,038,283 + BWI76_RS04945 0.60 -1.4
1,038,350 + BWI76_RS04945 0.65 +0.4
1,038,540 + BWI76_RS04945 0.77 -1.3
1,038,548 - BWI76_RS04945 0.77 +4.4
1,038,650 - BWI76_RS04945 0.84 -3.1
1,039,097 + -2.4
1,039,258 + BWI76_RS04950 0.11 +1.3
1,039,310 - BWI76_RS04950 0.13 +0.7
1,039,373 - BWI76_RS04950 0.16 +0.6
1,039,639 + BWI76_RS04950 0.27 +0.7
1,040,046 - BWI76_RS04950 0.44 +0.6
1,040,075 - BWI76_RS04950 0.45 +0.5
1,040,286 + BWI76_RS04950 0.54 -3.4
1,040,464 - BWI76_RS04950 0.62 -0.3
1,040,694 - BWI76_RS04950 0.71 -0.3
1,040,852 - BWI76_RS04950 0.78 +1.7
1,040,873 - BWI76_RS04950 0.79 -0.6
1,041,073 - BWI76_RS04950 0.87 -1.1
1,041,210 - -1.4
1,041,258 - -0.4
1,041,498 + -0.5
1,041,749 + BWI76_RS04955 0.31 +1.4
1,041,801 - BWI76_RS04955 0.40 +0.6
1,042,160 - -0.7
1,042,160 - -1.2
1,042,279 + BWI76_RS04960 0.16 +1.2
1,042,303 - -0.5

Or see this region's nucleotide sequence