Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04595

Experiment: D-Alanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04590 and BWI76_RS04595 are separated by 337 nucleotidesBWI76_RS04595 and BWI76_RS04600 are separated by 128 nucleotidesBWI76_RS04600 and BWI76_RS04605 overlap by 14 nucleotides BWI76_RS04590: BWI76_RS04590 - ribulokinase, at 954,731 to 956,440 _RS04590 BWI76_RS04595: BWI76_RS04595 - DNA-binding transcriptional regulator AraC, at 956,778 to 957,623 _RS04595 BWI76_RS04600: BWI76_RS04600 - inner membrane protein, at 957,752 to 958,519 _RS04600 BWI76_RS04605: BWI76_RS04605 - thiamine ABC transporter ATP-binding protein, at 958,506 to 959,207 _RS04605 Position (kb) 956 957 958Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 955.911 kb on - strand, within BWI76_RS04590at 956.018 kb on - strand, within BWI76_RS04590at 956.029 kb on + strand, within BWI76_RS04590at 956.139 kb on + strand, within BWI76_RS04590at 956.223 kb on - strand, within BWI76_RS04590at 956.310 kb on + strandat 956.352 kb on - strandat 956.484 kb on + strandat 956.487 kb on - strandat 956.539 kb on - strandat 956.749 kb on + strandat 956.908 kb on + strand, within BWI76_RS04595at 956.916 kb on - strand, within BWI76_RS04595at 956.921 kb on - strand, within BWI76_RS04595at 956.966 kb on + strand, within BWI76_RS04595at 957.063 kb on + strand, within BWI76_RS04595at 957.197 kb on - strand, within BWI76_RS04595at 957.217 kb on + strand, within BWI76_RS04595at 957.225 kb on - strand, within BWI76_RS04595at 957.302 kb on + strand, within BWI76_RS04595at 957.487 kb on - strand, within BWI76_RS04595at 957.491 kb on + strand, within BWI76_RS04595at 957.710 kb on - strandat 957.711 kb on + strandat 957.882 kb on + strand, within BWI76_RS04600at 957.910 kb on + strand, within BWI76_RS04600at 958.400 kb on + strand, within BWI76_RS04600

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Alanine (N)
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955,911 - BWI76_RS04590 0.69 -0.0
956,018 - BWI76_RS04590 0.75 +0.6
956,029 + BWI76_RS04590 0.76 +0.5
956,139 + BWI76_RS04590 0.82 -0.8
956,223 - BWI76_RS04590 0.87 -1.4
956,310 + -3.1
956,352 - +1.5
956,484 + -4.0
956,487 - -2.3
956,539 - +1.3
956,749 + +2.3
956,908 + BWI76_RS04595 0.15 +0.1
956,916 - BWI76_RS04595 0.16 +0.8
956,921 - BWI76_RS04595 0.17 +2.0
956,966 + BWI76_RS04595 0.22 -0.4
957,063 + BWI76_RS04595 0.34 +3.4
957,197 - BWI76_RS04595 0.50 -1.8
957,217 + BWI76_RS04595 0.52 -2.1
957,225 - BWI76_RS04595 0.53 -0.4
957,302 + BWI76_RS04595 0.62 -0.7
957,487 - BWI76_RS04595 0.84 +3.7
957,491 + BWI76_RS04595 0.84 +0.7
957,710 - +1.0
957,711 + -0.6
957,882 + BWI76_RS04600 0.17 -0.4
957,910 + BWI76_RS04600 0.21 -0.9
958,400 + BWI76_RS04600 0.84 +0.5

Or see this region's nucleotide sequence